回回甘松饮含药血清对高糖诱导的小鼠足细胞上皮间质转化相关信号通路的影响

发布时间：2018-05-26 16:49

本文选题：回回甘松饮 + 足细胞　；参考：《中国地方病防治杂志》2017年05期

【摘要】：目的研究回回甘松饮(HGY)对高糖诱导的小鼠足细胞TGF-β1、Smad3、Smad7基因及蛋白表达的影响。方法体外培养小鼠足细胞,分为正常对照组、高糖组、坎地沙坦(阳性)组、HGY高、中、低剂量组。采用酶联免疫吸附法检测TGF-β1、Smad3、Smad7的蛋白表达水平。结果高糖(25 mmol/L)使足细胞中TGF-β1、Smad3基因及蛋白表达量升高(P0.01),Smad7蛋白表达量下降(P0.01);HGY含药血清可下调高糖诱导的足细胞中TGF-β1、Smad3的蛋白表达量(P0.01或0.05),并使Smad7蛋白表达量升高(P0.01或0.05),且HGY与坎地沙坦含药血清的调控结果接近,无显著差异。结论 HGY含药血清可调控高糖环境中的足细胞中TGF-β1、Smad3、Smad7蛋白表达水平,这可能是其延缓早期糖尿病肾病所致肾纤维化进程的机制之一。
[Abstract]:Objective to study the effect of HGY on the expression of TGF- 尾 1 Smad3 Smad7 gene and protein in mouse podocytes induced by high glucose. Methods Mouse podocytes were cultured in vitro and divided into three groups: normal control group, high glucose group and high, middle and low dose group. The protein expression of TGF- 尾 _ 1 Smad3 and Smad7 was detected by enzyme-linked immunosorbent assay (Elisa). Results 25 mmol / L) increased the expression of TGF- 尾 1 Smad3 gene and protein in podocytes. The protein expression of TGF- 尾 1 and Smad3 in podocytes was decreased by P0. 01 or 0. 01% HGY. The protein expression of TGF- 尾 1 + Smad3 in podocytes induced by high glucose was down-regulated (P0.01 or 0. 05%), and the expression of Smad7 protein was increased (P0. 01 or 0. 05%) in podocytes, and the expression of TGF- 尾 1 and Smad3 in podocytes was increased by P0. 01 or 0. 05%. The regulatory results of HGY and candesartan serum were similar. There was no significant difference. Conclusion HGY serum can regulate the expression of TGF- 尾 1 and Smad3 Smad7 protein in podocytes in high glucose environment, which may be one of the mechanisms that delay the progression of renal fibrosis induced by early diabetic nephropathy.
【作者单位】：宁夏医科大学中医学院;宁夏医科大学药学院;宁夏医科大学回医药现代化省部共建教育部重点实验室;
【基金】：2015年宁夏自然科学基金项目(NZ15088)
【分类号】：R29

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