高效液相色谱法测定不同产地藏药诃子中7种鞣质类有效成分的含量

发布时间：2018-05-27 09:17

本文选题：高效液相色谱法 + 藏药诃子　；参考：《中国药学杂志》2017年12期

【摘要】：目的建立同时测定藏药诃子中没食子酸、诃子次酸、柯里拉京、没食子酸乙酯、诃子鞣酸、鞣花酸和五没食子酰葡萄糖的HPLC方法,测定不同产地诃子药材中7种鞣质类有效成分的含量。方法采用色谱柱为Hypersil ODS2 C_(18)(4.6 mm×250mm,5μm);流动相为乙腈(A)-0.05%甲酸水(B),梯度洗脱,紫外检测波长为290 nm;流速1 m L·min~(-1)。结果 7种有效成分线性关系良好(r0.999 8),加样回收率为95.2%~98.4%;8个产地不同品种的诃子中均含该7个被测成分,但含量差异大,其中诃子品种中海南、广西、广东和新疆几个产地的7种鞣质类有效成分的总含量较高;绒毛诃子品种中广东和广西两个产地的7种鞣质类有效成分的总含量较高。结论该方法快速简便,分离效果好,重复性好,可为诃子的质量控制提供重要参考。
[Abstract]:Objective to establish a HPLC method for the simultaneous determination of Gallic acid, chebulopyranic acid, Coriragin, ethyl gallate, xanthogalic acid, tannic acid and pentagnol glucose in the Tibetan medicine chebula. The contents of seven tannins were determined. Methods the chromatographic column was adopted as Hypersil ODS2 C _ (18) C _ (18) O _ (4.6) mm 脳 250 mm ~ (5) 渭 m 路m ~ (-1), the mobile phase was acetonitrile (A) -0.05% formic acid water (B _ (1), gradient elution, UV detection wavelength was 290nm, flow rate was 1 mL / min ~ (-1). Results the linear relationship of the seven active components was good, and the recovery rate was 95.20.98. 4%, the contents of 7 components were different in different varieties from 8 producing areas, among them, Hainan, Guangxi, the varieties of Fructus chebula were different, and the results showed that the content of the seven components was higher than that of the control group, but there was a significant difference in the content of the seven components. The total contents of seven kinds of tannins were higher in Guangdong and Xinjiang, and the total contents of seven kinds of tannins were higher in Guangdong and Guangxi. Conclusion the method is rapid, simple, effective and reproducible, and can provide important reference for the quality control of Fructus chebula.
【作者单位】：江西中医药大学药学院;
【基金】：江西民族传统药现代科技与产业发展协同创新中心2014年度协同创新项目资助(JXXT201403019) 江西省自然基金科学项目资助(20161BAB205222)
【分类号】：O657.72;R29

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