电针井穴对血管性痴呆大鼠海马CA1区p38MAPK的表达及细胞凋亡的影响

发布时间：2018-05-28 03:07

本文选题：电针 + 井穴　；参考：《安徽中医药大学》2016年硕士论文

【摘要】：目的:观察电针井穴对血管性血管性痴呆(VD)模型大鼠学习记忆能力及海马CA1区p38丝裂原活化蛋白激酶(p38MAPK)表达及细胞凋亡的影响,探讨电针井穴治疗VD的可能作用机制,为临床上针刺井穴治疗VD提供部分实验及理论依据。方法:1.选择清洁级成年雄性Wistar大鼠70只,体重(275±25)g,经1周适应性饲养后,为减少动物回避性反应差异造成的差异,用跳台实验筛除特别敏感和过于迟钝的大鼠,保留60只,电脑随机数字法选取假手术组8只,剩余大鼠根据改良的四血管阻断法(4-VO)制备VD模型,模型复制后4天,依据跳台实验及神经行为学评分判定模型是否复制成功,电脑随机数字法分为电针组、西药组、模型对照组3组,各8只。术后10天,电针组予以电针治疗,每天1次,10天为1个疗程,共3个疗程,西药组予以吡拉西坦灌胃治疗,每天1次,疗程与针刺组平行,模型对照组与假手术组常规饲养,不做特殊处理。2.疗程结束后先对各组大鼠进行跳台实验及神经行为学评分测定,然后取材,采用免疫组化法检测各组大鼠海马CA1区p38MAPK表达,Tunel法检测各组大鼠海马CA1区细胞凋亡。采用SPSS17.0对实验数据进行统计分析。结果:1.模型复制后第4d,跳台实验检测潜伏期、错误次数,同时检测神经行为学评分。结果:模型复制组与假手术组相比潜伏期降低(P0.01),错误次数增多(P0.05),神经行为学评分增高(P0.01),表明模型复制成功。2.疗程结束后,再次检测各组大鼠跳台实验潜伏期、错误次数及神经行为学评分。结果:结果模型对照组与电针组相比潜伏期降低(P0.01),错误次数增多(P0.05),神经行为学评分增高(P0.05),差距有统计学意义;模型对照组与西药组相比潜伏期降低(P0.05),差异有统计学意义,错误次数增多(P0.05),神经行为学评分(P0.05),差异无统计学意义,电针组与西药组相比潜伏期延长(P0.05),错误次数减少(P0.05),神经行为学评分(P0.05),差异无统计学意义。结果表明电针组能明显改善VD大鼠的学习记忆能力、神经行为学评分,且电针组在改善学习记忆能力方面优与西药组。3.治疗结束后,免疫组化法检测各组大鼠海马CA1区p38MAPK表达。与模型对照组相比,假手术组平均灰度值增高(P0.01),阳性细胞表达减少;模型对照组与西药组相比,平均灰度值降低(P0.01),差异有统计学意义,与电针组相比平均灰度值减少(P0.01),阳性细胞表达增多;西药组与电针组相比,灰度值(P0.05),差异无统计学意义;电针组与假手术组相比,平均灰度值增高(P0.05),结果表明电针组能降低海马CA1区p38MAPK的阳性表达。4.Tunel法检测海马CA1区细胞凋亡,假手术组与模型对照组相比,细胞凋亡指数明显降低(P0.01),差异有统计学意义,凋亡程度明显减轻;电针组与模型对照组相比,细胞凋亡程度较轻(P0.01),差异有统计学意义;西药组与模型对照组比较,细胞凋亡程度减轻(P0.01),差异有统计学意义;电针组与假手术相比,细胞凋亡指数增高(P0.01),差异有统计学意义,西药组与假手术组相比,细胞凋亡指数增高(P0.01),差异有统计学意义。西药组与电针组相比,细胞凋亡指数增高(P0.01),差异有统计学意义,结果表明电针井穴能降低海马CA1区细胞凋亡指数,减少神经元细胞凋亡,且疗效优于西药组。结论:1.电针井穴能改善VD大鼠学习记忆能力,降低VD大鼠神经行为学评分。2.电针井穴能抑制p38MAPK的表达,提示电针井穴治疗VD可能是通过抑制p38MAPK信号通道,减轻神经元细胞凋亡。3.电针井穴能减轻VD大鼠海马CA1区细胞凋亡,减轻继发性损伤,促进神经元的修复。
[Abstract]:Objective: To observe the effects of Electroacupuncture on the learning and memory ability of vascular dementia (VD) model rats and the expression of p38 mitogen activated protein kinase (p38MAPK) and apoptosis in hippocampus CA1 region, and to explore the possible mechanism of the treatment of VD by Electroacupuncture well points, and to provide some experimental and theoretical basis for the treatment of VD in clinical acupuncture points. Method: 1. 70 adult male Wistar rats were selected, weight (275 + 25) g, after 1 weeks of adaptive feeding, to reduce the difference of the difference in the difference of animal avoidance response. 60 rats were retained, 8 rats were selected by the random number method of computer, and the remaining rats were selected according to the improved four vascular blocking method (4- VO) VD model was prepared. 4 days after the replicating of the model, the model was made to determine whether the model was replicated successfully. The computer random number method was divided into the electroacupuncture group, the western medicine group and the model control group, each 8. The electroacupuncture group was treated with electroacupuncture at 10 days after the operation, 1 times a day, and 1 courses for 10 days, and the western medicine group was given Laci Staw. 1 times a day, 1 times a day, the treatment course was parallel to the acupuncture group. The model control group and the sham operation group were reared routinely. After the special treatment of the.2. course, the rats were tested on the platform of the platform and the neurobehavioral score was measured. Then the materials were obtained by immunohistochemistry. The expression of p38MAPK in the hippocampal CA1 area of the rats was detected by immunohistochemistry. The Tunel method was used to detect the rats in each group. Hippocampal CA1 region cell apoptosis. The results of the experimental data were statistically analyzed with SPSS17.0. Results: 1. after replicating of the model 4D, the incubation period was detected, the number of errors and the neurobehavioral score were detected. Results: the incubation period of the model replicating group was lower than the sham group (P0.01), the number of errors increased (P0.05), and the neurobehavioral score increased (P 0.01), after the successful.2. course of the model replication, the incubation period, the error times and the neurobehavioral score of the rats were detected again. Results: the results showed that the incubation period of the model control group was lower (P0.01), the number of errors increased (P0.05), the neurobehavioral score increased (P0.05), and the difference was statistically significant; model control Compared with the western medicine group, the latency decreased (P0.05), the difference was statistically significant, the number of errors increased (P0.05), the neurobehavioral score (P0.05), the difference was not statistically significant, the incubation period of the electroacupuncture group and the western medicine group was prolonged (P0.05), the number of errors decreased (P0.05) and the neurobehavioral score (P0.05), the difference was not statistically significant. The results showed that the electroacupuncture was not significant. The group can significantly improve the learning and memory ability of VD rats, neurobehavioral score, and the improvement of learning and memory ability in the electroacupuncture group and the western medicine group.3. treatment after the end of the western medicine group, immunohistochemical method to detect the expression of p38MAPK in the hippocampus CA1 area of rats in each group. Compared with the model control group, the average gray value of the sham operation group increased (P0.01), and the expression of positive cells decreased. Compared with the western medicine group, the average gray value of the model control group was reduced (P0.01), the difference was statistically significant, the average gray value decreased (P0.01) compared with the electroacupuncture group, and the expression of positive cells increased. The difference of gray value (P0.05) in the western medicine group and the electroacupuncture group was not statistically significant; the average gray value of the electroacupuncture group was higher than the sham operation group (P0.05), the result table was higher than that of the sham operation group (P0.05). The positive expression of p38MAPK in the hippocampal CA1 area was reduced by the positive electroacupuncture group. The apoptosis index of the hippocampal CA1 region was detected by.4.Tunel method. Compared with the model control group, the apoptosis index decreased significantly (P0.01), the difference was statistically significant and the degree of apoptosis was significantly reduced. Compared with the model control group, the apoptosis degree was less than that of the model control group (P0.01), and there were differences in the cell apoptosis degree (P0.01). Statistical significance: compared with the model control group, the degree of apoptosis was reduced (P0.01) in the western medicine group and the difference was statistically significant. The apoptosis index increased (P0.01) in the electroacupuncture group compared with the sham operation (P0.01), and the difference was statistically significant in the western medicine group and the sham operation group (P0.01), the difference was statistically significant. Compared to the group, the apoptosis index increased (P0.01), the difference was statistically significant. The results showed that the electroacupuncture hole can reduce the apoptosis index of CA1 area in the hippocampus and reduce the apoptosis of neuron cells, and the effect is better than that of the western medicine group. Conclusion: 1. electroacupuncture well can improve the learning and memory ability of VD rats and reduce the neurobehavioral score of VD rats by.2. electroacupuncture well. The expression of p38MAPK suggests that the treatment of VD by Electroacupuncture well points may be by inhibiting the p38MAPK signal channel and reducing the apoptosis of.3. electroacupuncture at the neuronal cell apoptosis, reducing the apoptosis in the CA1 region of the hippocampus of VD rats, reducing secondary damage and promoting the repair of neurons.
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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