槲寄生滴眼液治疗兔围绝经期干眼症的应用研究

发布时间：2018-06-03 17:29

  本文选题：槲寄生滴眼液 + 治疗　； 参考：《中国医院药学杂志》2017年09期

【摘要】：目的:探究槲寄生滴眼液对治疗围绝经期雌兔干眼症的效果。方法:取健康雌兔36只将24只雌兔行双侧卵巢切除术,处理后,将这24只受试兔按照随机数字表法分为2组(每组12只,均为右眼):对生理盐水组(A组)、槲寄生滴眼液组(B组),连续滴眼2个月,每日4次。剩余的12只雌兔(C组)打开腹腔,不切除卵巢,眼部不进行任何处理,作为假手术组。分别于手术前、手术后1周、2周、1个月及2个月对受试兔行基础泪液分泌(SIT)检查、角膜荧光素染色(FL)评分、泪液蛋白测定及角膜共聚焦显微镜扫描。结果:A、B2组干预前SIT、FL相比,差异无统计学意义(P0.05),与假手术组比较,差异均有统计学意义(均P0.05);干预2个月后,假手术组SIT、FL评分较干预前没有明显变化(P0.05),而A、B组SIT、FL评分较干预前变化明显,差异均有统计学意义(P0.05)。干预2个月后,B组与假手术组相比,差异无统计学意义(P0.05),而A组与假手术组相比,差异有统计学意义(P0.05)。A、B2组各时间点SIT、FL评分相比,差异均有统计学意义(P0.05)。A、B2组干预2个月后泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性相比,差异均有统计学意义(P0.05)。经角膜共焦显微镜显示:B组角膜上皮下神经及角膜基质层神经干较直,与正常雌兔上皮下神经比较数量稍有减少。而A组角膜上皮下神经及角膜基质层神经干弯曲,数量明显减少。结论:槲寄生滴眼液可有效减轻围绝经期雌兔干眼症的症状和体征,应用于围绝经期女性干眼症治疗有一定的临床意义。
[Abstract]:Objective: to investigate the effect of mistletoe eye drops on peri-menopausal female rabbit dry eye. Methods: after bilateral ovariectomy was performed on 24 healthy female rabbits, the 24 rabbits were randomly divided into 2 groups (12 rabbits in each group). All of them were right eye: group A in normal saline group and group B in mistletoe eye drops were dripped continuously for 2 months, 4 times a day. The remaining 12 female rabbits were treated as sham-operated group. Before operation, 1 week, 1 month and 2 months after operation, the basic tear secretion test, corneal fluorescein staining (FLL) score, tear protein measurement and corneal confocal microscope scanning were performed. Results there was no significant difference between the two groups before intervention (P 0.05), but there was significant difference between the two groups (all P 0.05), and after 2 months of intervention, there was no significant difference between the two groups (P 0.05), and there was no significant difference between the control group and sham operation group (P 0.05). There was no significant change in the score of SITF in sham operation group compared with that before intervention (P 0.05), but the score of SITF in group A was significantly different from that in group A (P 0.05). Two months after intervention, there was no significant difference between group B and sham operation group (P 0.05), but the difference between group A and sham group was statistically significant. There were significant differences in the total protein content, lactoferrin, lysozyme and amylase activity in tears after two months of intervention. The corneal confocal microscopy showed that the corneal subepithelial nerve and the corneal stromal layer nerve trunk in group B were straight, and the number of the subcutaneous nerves was slightly decreased compared with the normal female rabbits. In group A, the number of corneal subcutaneous nerve and corneal stromal nerve trunk was significantly reduced. Conclusion: mistletoe eye drops can effectively relieve the symptoms and signs of peri-menopausal female rabbit dry eye, and it has certain clinical significance in the treatment of peri-menopausal female dry eye.
【作者单位】： 江西省人民医院眼科临床营养科;南昌大学第一附属医院眼科;厦门大学眼科研究所;
【基金】：国家自然科学基金资助(编号:81660158,81400372) 江西省自然科学基金(编号:20151BAB215016) 江西省科技支撑计划项目(编号:20151BBG70223) 江西省卫生计生委科技项目(编号:20161008)
【分类号】：R271.116
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本文编号：1973604