哈尼族验方“莫那奇”对脑出血大鼠脑组织BDNF表达影响的研究

发布时间：2018-06-03 19:09

本文选题：哈尼族医药 + 莫那奇　；参考：《云南中医学院》2017年硕士论文

【摘要】：研究目的通过对脑源性神经营养因子(BDNF)表达水平等指标的检测,研究哈尼族验方“莫那奇”对脑出血的治疗作用,探讨“莫那奇”在脑出血治疗中的作用机制。实验方法将72只SD大鼠随机分为空白组、假手术组、模型组和莫那奇组,采用尾状核自体血注入法复制大鼠脑出血模型,在造模3小时后及“莫那奇”干预后第3天按照Berderson行为学评分法对大鼠神经缺损体征进行评分并处死取材,应用干湿重法测定其脑组织含水量、HE染色法观察脑组织形态学改变情况及用原位杂交方法检测干预后BDNF阳性细胞数量的变化。实验结果1.脑出血3小时后,模型组和莫那奇组均表现为出血对侧肢体瘫痪,空白组和假手术组大鼠基本无神经功能异常表现。经“莫那奇”干预3天后大鼠神经缺损体征有所改善,与模型组及治疗前相比,差异具有统计学意义(P0.05)。2.干湿重法结果显示,模型组和莫那奇组脑组织含水量较空白组和假手术组显著升高(P0.01),其中应用“莫那奇”治疗后大鼠组脑组织含水量较模型组降低(P0.05)。3.HE染色显示,模型组大鼠脑组织出血灶及周围细胞大多坏死变性,胞质内有较多含铁血黄素颗粒,细胞核浓缩,染色质崩解、分散于胞浆内,炎性细胞浸润,胶质细胞形成纤维囊肿,散在于血肿周围,莫那奇组与模型组相比细胞结构较规整,排列较整齐,炎性细胞较少,空白组和假手术组组织及细胞结构完整,无明显病理改变。4.原位杂交结果显示莫那奇组BDNF阳性细胞数显著多于模型组(P0.01),空白组和假手术组基本无BDNF阳性细胞。结论实验证实哈尼族验方“莫那奇”能够改善大鼠神经缺损体征、降低脑组织含水量、保护脑组织及神经元的形态结构、促进脑出血大鼠脑内脑源性神经营养因子BDNF的表达,对脑出血后的脑组织损伤发挥重要的保护作用。
[Abstract]:Objective to study the therapeutic effect of Monaci on intracerebral hemorrhage (ICH) by detecting the expression level of BDNF, and to explore the mechanism of Monachi in the treatment of intracerebral hemorrhage (ICH). Methods Seventy-two Sprague-Dawley rats were randomly divided into three groups: blank group, sham operation group, model group and monazide group. The rat model of intracerebral hemorrhage was induced by autologous blood injection of caudate nucleus. After 3 hours of model making and 3 days after "Monachy" intervention, the rats were scored according to the Berderson behavioral score method, and the rats were killed to take the materials. The changes of brain tissue morphology and the number of BDNF positive cells after intervention were observed by using dry and wet weight method and HE staining. Experimental results 1. After 3 hours of intracerebral hemorrhage, the model group and Monachy group showed the contralateral limb paralysis, while the blank group and the sham operation group showed no abnormal neurological function. The physical signs of nerve defect in rats were improved after 3 days of "monagi" intervention, and the difference was statistically significant compared with the model group and before treatment (P0.05. 2). The results of dry and wet weight method showed that the water content of brain tissue in the model group and monazi group was significantly higher than that in the blank group and sham-operation group, and the water content of brain tissue in the model group was lower than that in the model group after the treatment of "monagi". 3. The results of HE staining showed that the water content of brain tissue in the model group was lower than that in the model group. In the model group, most of the cerebral hemorrhage foci and peripheral cells were necrotic and denatured, and there were more hemosiderin granules, nuclear concentration, chromatin disintegration, dispersion in the cytoplasm, infiltration of inflammatory cells and formation of fibrocysts in glial cells in the model group. Compared with the model group, the monaqi group had more regular cell structure, neatly arranged and fewer inflammatory cells. The tissue and cell structure of the blank group and the sham operation group were intact, and there were no obvious pathological changes. 4. The results of in situ hybridization showed that the number of BDNF positive cells in Monachi group was significantly higher than that in model group (P 0.01), and there were no BDNF positive cells in blank group and sham operation group. Conclusion Monaci can improve the signs of nerve defect, decrease the water content of brain tissue, protect the morphologic structure of brain tissue and neuron, and promote the expression of brain-derived neurotrophic factor (BDNF) in the brain of rats with intracerebral hemorrhage. It plays an important role in protecting brain tissue injury after intracerebral hemorrhage.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29

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