电针内关穴预处理对大鼠心肌缺血再灌注后线粒体功能及超微结构的保护作用

发布时间：2018-06-08 01:34

本文选题：电针 + 预处理　；参考：《中华中医药杂志》2017年10期

【摘要】：目的:探讨电针内关穴预处理对心肌缺血再灌注损伤过程中心肌线粒体的保护作用。方法:选用3月龄Wistar雄性大鼠30只,体质量(220±28)g,编号后按随机数字表分入空白组、模型组、电针预处理组,每组10只。造模采用结扎左冠状动脉前降支,缺血30min,再灌注2h,随即处死大鼠,留取左心室心肌组织,采用黄嘌呤氧化法检测心肌超氧化物歧化酶(SOD)活性,硫代巴比妥酸法检测心肌丙二醛(MDA),通过极谱法检测系统测定线粒体呼吸控制率(RCR),透射电镜下观察心肌超微组织的变化。结果:电针预处理组大鼠部分心肌肌丝溶解、断裂,心肌细胞轻微肿胀,轻度空化,线粒体排列稍紊乱,部分线粒体水肿、存在空泡样病变。电针预处理组的大鼠线粒体RCR、SOD及ATP含量明显高于模型组(P0.01),MDA含量明显低于模型组(P0.01)。结论:电针内关穴预处理能改善心肌组织形态学表达,防止心肌组织结构的破坏,修护心肌缺血性损伤,更对线粒体的结构与功能起到了良性的保护作用。
[Abstract]:Aim: to investigate the protective effect of electroacupuncture at Neiguan point on myocardial mitochondria during myocardial ischemia reperfusion injury. Methods: thirty 3-month-old male Wistar rats with body weight of 220 卤28g were divided into blank group, model group and electroacupuncture preconditioning group (10 rats in each group). The model was established by ligating the anterior descending branch of the left coronary artery, ischemia for 30 min, reperfusion for 2 h, then the rats were killed and left ventricular myocardial tissue was retained. The activity of myocardial superoxide dismutase (SOD) was measured by xanthine oxidation method. The myocardial malondialdehyde (MDA) MDAA was detected by thiobarbituric acid method, the mitochondrial respiratory control rate (RCRR) was measured by polarographic method, and the changes of myocardial ultrastructure were observed under transmission electron microscope (TEM). Results: in electroacupuncture preconditioning group, some myofilaments were dissolved and broken, myocardial cells were slightly swollen, slightly cavitation, mitochondria arrangement was slightly disordered, some mitochondria were edema, and vacuolar lesions existed. The contents of RCR-SOD and ATP in mitochondria in electroacupuncture preconditioning group were significantly higher than those in model group (P 0.01) and the content of MDA was significantly lower than that in model group (P 0.01). Conclusion: electroacupuncture at Neiguan point preconditioning can improve the expression of myocardial morphology, prevent the destruction of myocardial structure, repair myocardial ischemic injury, and play a benign role in protecting the structure and function of mitochondria.
【作者单位】：湖北中医药大学针灸骨伤学院针灸治未病湖北省协同创新中心;
【基金】：国家自然科学基金青年科学基金项目(No.81403460) 湖北中医药大学教育教学研究课题(No.2014A06)~~
【分类号】：R245.97

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