针刺对高脂饮食诱导的肥胖小鼠白色脂肪组织巨噬细胞极化的影响

发布时间：2018-06-10 06:53

本文选题：肥胖 + 针刺　；参考：《中国针灸》2017年11期

【摘要】：目的:观察穴位与非穴位针刺对高脂饮食诱导的肥胖小鼠白色脂肪组织巨噬细胞极化的影响。方法:将50只雄性C 57 BL/6小鼠随机抽取10只作为正常组,其余40只给予持续高脂饮食造模16周,将30只造模成功的小鼠随机分为模型组、非穴位组、穴位组,每组10只,继续给予高脂饮食喂养8周。正常组继续给予普通饲料喂养8周。自造模成功后第2天开始,穴位组小鼠针刺"关元""足三里""胃脘下俞",非穴位组针刺尾部2个非穴位,每日1次,每次针刺15 min,连续治疗8周。于造模成功后及末次治疗后第2天记录各组小鼠体质量;眼眶采血,检测小鼠血清三酰甘油(triglyceride,TG)、血清总胆固醇(total cholesterol,TC)水平;HE染色观察各组小鼠附睾白色脂肪组织形态,real-time quantitative PCR(RT-q PCR)检测小鼠附睾白色脂肪中白细胞介素6(interleukin-6,IL-6)、单核细胞趋化蛋白-1(monocyte chemotactic protein 1,MCP-1)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素10(interleukin-10,IL-10)、诱导型一氧化氮合酶(inducible nitric oxide synthesis,i NOS)和CD 206 m RNA表达情况,免疫组化(IHC)检测附睾白色脂肪组织中i NOS和CD 206的蛋白表达情况。结果:与正常组比较,模型组、非穴位组、穴位组小鼠在喂养第16周和第24周时体质量均明显上升(均P0.05);与模型组比较,穴位组小鼠在第24周时体质量明显降低(P0.05)。与正常组比较,模型组TG、TC明显上升(均P0.05);与模型组比较,非穴位组TC明显降低(P0.05),穴位组TG、TC明显降低(均P0.05)。与正常组比较,模型组和非穴位组小鼠脂肪组织中MCP-1、TNF-α、IL-6、i NOS m RNA的表达明显升高(均P0.05),IL-10、CD 206 m RNA的表达明显降低(均P0.05);与模型组比较,穴位组脂肪组织中MCP-1、TNF-α、IL-6、i NOS m RNA的表达明显降低(均P0.05),IL-10、CD 206 m RNA的表达明显升高(均P0.05)。HE染色可见:正常组脂肪组织呈蜂窝状结构,由大量单泡脂肪细胞构成,脂肪细胞呈多边形或圆形,呈空泡状;模型组脂肪细胞明显增大且脂肪细胞大小不规则,细胞间隙变大;穴位组脂肪细胞变小,细胞间隙变小。与正常组比较,模型组和非穴位组小鼠脂肪组织中i NOS蛋白表达明显升高(均P0.05),CD206蛋白表达明显降低(均P0.05);与模型组比较,穴位组脂肪组织中i NOS蛋白表达明显降低(P0.05),CD206蛋白表达明显升高(P0.05)。结论:针刺"关元""足三里""胃脘下俞"穴可通过影响白色脂肪组织巨噬细胞极化改善肥胖小鼠脂肪炎性反应。
[Abstract]:Aim: to observe the effect of acupuncture at acupoints and non-acupoints on the polarization of macrophages in white adipose tissue of obese mice induced by high fat diet. Methods: ten male C57BL / 6 mice were randomly selected as normal group, the other 40 mice were given continuous high-fat diet for 16 weeks, and 30 successful mice were randomly divided into model group (n = 10), non-acupoint group (n = 10) and control group (n = 10). Continue feeding on a high fat diet for 8 weeks. The normal group was given normal feed for 8 weeks. Since the second day after the successful establishment of the model, acupuncture of "Guanyuan" Zusanli "Weiwan Xiazu" in the acupoint group and 2 non-acupoints in the non-acupoint group were treated continuously for 8 weeks, once a day for 15 mins each time. The body mass of each group was recorded on the second day after the successful establishment of the model and the second day after the last treatment. Detection of serum triglyceride (TGG) and total cholesterol (TC) in mice by HE staining to observe the morphology of white adipose tissue in the epididymis of each group and to detect interleukin-6 interleukin-6mil and monocyte chemoattractant protein -1 monocyte in white fat of mouse epididymis The expression of TNF- 伪, IL-10, inducible nitric oxide synthase synthesis-inducible nitric oxide synthesis-iNOS) and CD206mRNA were detected by chemotactic protein 1, tumor necrosis factor 伪, TNF- 伪, interleukin-10, inducible nitric oxide synthesizer and CD206mRNA, respectively. The expression of iNOS and CD206 in white adipose tissue of epididymis was detected by immunohistochemistry (IHC). Results: compared with the normal group, the body weight of the model group, the non-point group and the acupoint group increased significantly at the 16th and 24th week of feeding (all P 0.05), and the body mass of the acupoint group decreased significantly at the 24th week compared with the model group. Compared with the normal group, the TGG TC in the model group was significantly higher than that in the model group (all P0.05A), and that in the non-acupoint group was significantly lower than that in the non-acupoint group, and in the acupoint group, the TC in the acupoint group was significantly lower than that in the model group (all P 0.05). Compared with the normal group, the expression of NOS mRNA in the adipose tissue of the model group and non-acupoint group was significantly higher than that in the model group (P0.05, P 0.05) and the expression of CD206 mRNA was significantly lower in the model group than that in the model group, and compared with the model group, the expression of IL-10 + CD206 mRNA in the model group was significantly lower than that in the control group. In adipose tissue of acupoint group, the expression of MCP-1TNF- 伪 IL-6i NOS mRNA was significantly decreased (P0.05 + IL-10 + CD206 mRNA expression was significantly increased (P0.05). He staining showed that normal adipose tissue was honeycombed and consisted of a large number of single follicular adipocytes. The adipocytes were polygonal or round and vacuolated; the adipocytes in the model group were obviously enlarged and the size of the adipocytes was irregular and the gap between the adipocytes became larger; in the acupoint group the adipocytes became smaller and the intercellular space became smaller. Compared with the normal group, the expression of iNOS protein in the adipose tissue of the model group and the non-acupoint group was significantly increased (P 0.05) and the expression of CD206 was significantly decreased (all P 0.05), and compared with the model group, the expression of iNOS was significantly higher than that of the control group. The expression of iNOS protein in adipose tissue of acupoint group decreased significantly. Conclusion: acupuncture at "Guanyuan", "Zusanli" and "Weiwan Xiashu" can improve the fat inflammatory response of obese mice by affecting the polarization of macrophages in white adipose tissue.
【作者单位】：湖北中医药大学针灸骨伤学院;湖北中医药大学基础医学院;湖北中医药大学针灸骨伤学院针灸治未病湖北省协同创新中心;
【基金】：湖北省教育厅科学研究计划重点项目:D 20142006
【分类号】：R245

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