复元活血汤干预大鼠坐骨神经吻合术后神经修复的研究

发布时间：2018-06-13 10:39

本文选题：复元活血汤 + 血管内皮成长因子　；参考：《皖南医学院》2016年硕士论文

【摘要】：目的:考察复元活血汤对大鼠坐骨神经吻合术后神经修复的影响,并探讨其可能机制。方法:120只SD大鼠随机分为4组:1生理盐水组:右坐骨神经离断后缝合,灌胃时给予生理盐水。2甲钴胺组:右坐骨神经离断后缝合,灌胃时给予甲钴胺混悬液,浓度为0.2mg/ml。3复原活血汤组:右坐骨神经离断后缝合,术后复原活血汤灌胃。复元活血汤冻干粉灌胃剂量为0.18g·100g-1·d。4假手术组:右坐骨神经暴露后不做任何处理关闭伤口。治疗2周后Western Blot检测神经吻合口上下2cm神经组织VEGF、TGFβ1、SRC与p-SRC、ERK以及p-ERK蛋白的表达。2周、4周、8周对大鼠小腿三头肌行肌电图分析。治疗8周后坐骨神经吻合术后3组,荧光金示踪观察大鼠脊髓背跟神经节免疫荧光阳性细胞数。同时对坐骨神经和支配的肌肉HE染色后观察有无差异.结果:1.术后2周,复元活血汤组吻合口神经组织的VEGF蛋白水平显著高于生理盐水组,也高于甲钴胺组。2.术后2周,复元活血汤组吻合口神经组织的P-ERK的表达与生理盐水组有显著差异(P0.05),复原活血汤组与甲钴胺组之间差异无统计学意义(P0.05)。3.术后2周,复元活血汤组吻合口神经组织TGFβ1的表达高于生理盐水组(P0.05),但低于甲钴胺组(P0.05)。4.术后8周,较之生理盐水组,甲钴胺组和复元活血汤组大鼠脊髓背根神经节免疫荧光阳性细胞数显著增加;在坐骨神经功能指数检查中,甲钴胺组、复元活血汤组与生理盐水组的坐骨神经功能指数有明显差异(P0.01),甲钴胺组与复元活血汤组无明显差异(P0.05)。结论:复元活血汤通过诱导吻合口神经组织VEGF的分泌,活化VEGF/ERK/TGFβ1信号通路,促进损伤坐骨神经的修复。
[Abstract]:Aim: to investigate the effect of Fuyuan Huoxue decoction on nerve repair after sciatic nerve anastomosis in rats and to explore its possible mechanism. Methods one hundred and twenty Sprague-Dawley rats were randomly divided into 4 groups: the right sciatic nerve was sutured after the right sciatic nerve was severed and the right sciatic nerve was sutured after the right sciatic nerve was severed. The concentration was 0.2mg/ml.3 recovery Huoxue decoction group: right sciatic nerve was sutured after dissection, and postoperative recovery Huoxue decoction was perfused into the stomach. The dosage of Fuyuan Huoxue Tang freeze-dried powder was 0.18g 100g-1 d.4 sham-operation group: the right sciatic nerve was exposed without any treatment to close the wound. After 2 weeks of treatment, the expressions of VEGF TGF- 尾 1 SRC, p-SRCnERK and p-ERK protein were detected by Western blot. 2 weeks and 8 weeks after treatment, the electromyography of triceps muscle of the leg of rats was analyzed. After 8 weeks of sciatic nerve anastomosis, the number of immunofluorescence positive cells in spinal dorsal calcaneal ganglion (DRG) of rats was observed by fluorescent gold tracer. At the same time, he staining of sciatic nerve and innervated muscle was observed. The result is 1: 1. 2 weeks after operation, the level of VEGF protein in anastomotic nerve tissue of Fuyuan Huoxue decoction group was significantly higher than that of normal saline group and mecobalamin group. 2 weeks after operation, the expression of P-ERK in anastomotic nerve tissue in Fuyuan Huoxue Tang group was significantly different from that in normal saline group (P 0.05). At 2 weeks after operation, the expression of TGF 尾 1 in anastomotic nerve tissue of Fuyuan Huoxue Tang group was higher than that of normal saline group (P 0.05), but lower than that of mecobalamin group (P 0.05). At 8 weeks after operation, the number of immunofluorescence positive cells in spinal dorsal root ganglion was significantly increased in Mecobalamin group and Fuyuan Huoxue decoction group, and in the sciatic nerve function index test, the number of Mecobalamin group was significantly higher than that of normal saline group. There was significant difference in sciatic nerve function index between Fuyuan Huoxue decoction group and normal saline group, but there was no significant difference between Mecobalamin group and Fuyuan Huoxue decoction group. Conclusion: Fuyuan Huoxue decoction can promote the repair of injured sciatic nerve by inducing the secretion of VEGF in anastomotic nerve tissue and activating the signal pathway of VEGF / ERK / TGF- 尾 _ 1.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R277.7

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