2型糖尿病脾虚证患者血清microRNA表达谱和生物信息学分析（英文）

发布时间：2018-06-13 18:27

本文选题：脾虚证 + 型糖尿病　；参考：《中华中医药杂志》2017年08期

【摘要】：目的:通过对2型糖尿病脾虚证患者血清micro RNA(miRNA)表达谱和生物信息学分析,从miRNA水平上探讨脾虚证的发病机制和辨证分型。方法:选择5例2型糖尿病脾虚证患者和5名健康志愿者,从他们的血清标本中提取RNA,进行miRNA定量PCR芯片实验。筛选出两组之间差异表达的miRNA,利用miRSystem数据库进行靶基因预测和功能注释。结果:在2型糖尿病脾虚证患者和健康志愿者的血清中发现16个差异表达的miRNA。聚类分析显示,这16个差异表达的miRNA能够将2型糖尿病脾虚证患者和健康志愿者很好的区分。靶基因预测和功能注释显示,14个上调miRNA的调控122个靶基因显著富集到6个KEGG通路和6个GO分子功能。这些富集的KEGG通路包括与脂肪酸、氨基酸和2型糖尿病相关的营养物质代谢通路,钙离子信号通路,致病的大肠杆菌感染通路和亨廷顿氏舞蹈病通路。这些富集的GO分子功能包括羧酸、脂类、辅因子和药物结合功能,和酶激活剂和转录抑制剂活性功能。然而,2个下调miRNA调控的10个靶基因显著富集到2个KEGG通路。它们是内质网中的蛋白质加工通路和志贺氏菌病通路。结论:本研究可能为2型糖尿病脾虚证患者的临床辨证提供了一些血清miRNA标志物,同时从miRNA水平上为脾虚证患者的发病机制提供了依据。
[Abstract]:Objective: to investigate the pathogenesis and syndrome differentiation of spleen deficiency syndrome by analyzing the expression profile and bioinformatics of serum micro RNA in type 2 diabetic patients with spleen deficiency syndrome. Methods: five patients with type 2 diabetes mellitus with spleen deficiency syndrome and five healthy volunteers were selected to extract RNAs from their serum samples and carry out miRNA quantitative PCR chip experiments. The differentially expressed miRNAs between the two groups were screened, and the target gene prediction and functional annotation were carried out by using the miRSystem database. Results: sixteen differentially expressed miRNAs were found in the sera of type 2 diabetic patients with spleen deficiency syndrome and healthy volunteers. Cluster analysis showed that the 16 differentially expressed miRNAs could distinguish between type 2 diabetes mellitus patients with spleen deficiency syndrome and healthy volunteers. Target gene prediction and functional annotation showed that 14 up-regulated miRNA-regulated 122 target genes were significantly enriched to 6 KEGG pathways and 6 go molecules. These enriched KEGG pathways include nutrient metabolic pathways associated with fatty acids, amino acids and type 2 diabetes, calcium signaling pathways, pathogenic Escherichia coli infection pathways and Huntington's chorea pathway. These enriched go molecular functions include carboxylic acids, lipids, cofactors and drug binding functions, and enzyme activators and transcription inhibitor activities. However, 10 target genes down-regulated miRNA were significantly enriched into 2 KEGG pathways. They are protein processing pathway and Shigella pathway in endoplasmic reticulum. Conclusion: this study may provide some serum miRNA markers for the patients with spleen deficiency syndrome in type 2 diabetes mellitus and provide evidence for the pathogenesis of spleen deficiency syndrome from the level of miRNA.
【作者单位】：广东药科大学基础学院生物化学与分子生物学系;广东药科大学附属第一医院中医科;广州中医药大学脾胃研究所;广东药科大学附属第一医院检验科;
【基金】：National Natural Science Foundation of China(No.81102703) Science and Technology Planning Project of Guangdong Province(No.2013A032500005) Administration of Traditional Chinese Medicine of Guangdong Province(No.20123001) Natural Science Foundation for Fostering of Guangdong Pharmaceutical University(No.GYFYLH201303)~~
【分类号】：R259

【参考文献】