巨刺对急性脑缺血再灌注损伤大鼠神经干细胞增殖的影响

发布时间：2018-06-15 09:50

本文选题：脑缺血再灌注 + 神经干细胞　；参考：《湖南中医药大学》2016年硕士论文

【摘要】：目的:观察巨刺对急性脑缺血再灌注损伤大鼠神经干细胞增殖的影响,探讨其治疗缺血性脑血管病的作用机制。方法:将50只SD大鼠随机分为空白组10只、假手术组10只、造模组30只,空白组为正常对照组;假手术组向颈内动脉插入线栓5mm,假造模;造模组采用改良的longa线栓法制备MCAO缺血再灌注损伤大鼠模型,大鼠苏醒后予以神经神经功能评分,将造模成功后大鼠再次随机分为模型组、患侧针刺组、巨刺组,每组10只。空白组、假手术组、模型组不予处理;患侧针刺组、巨刺组于造模后24h行相应的针刺及电针干预,每日一次,共干预7次。实验结束后,对各组大鼠进行神经功能缺损评分,光镜下观察大鼠大脑皮层组织形态学结构,采用免疫组化结合图像分析检测大鼠缺血侧海马区Brd U阳性细胞数和Nestin平均光密度值,从而评价巨刺对急性脑缺血再灌注脑损伤大鼠神经干细胞的增殖作用。结果:1.神经功能缺损评分:造模后,大鼠均出现神经功能缺损;干预结束后,与模型组比较,患侧针刺组、巨刺组神经功能缺损评分降低,差异有统计学意义(P0.05,P0.01);与患侧针刺组比较,巨刺组的评分差异未达到统计学意义(P0.05)。2.脑组织形态结构:与模型组、患侧针刺组比较,巨刺组大鼠脑组织破坏程度减轻,细胞及血管周围间隙减小,毛细血管增多。3.神经干细胞增殖结果:与空白组、假手术组比较,模型组大鼠Brd U阳性细胞数增多、Nestin平均光密度值增大,差异有统计学意义(P0.01);与模型组比较,患侧针刺组、巨刺组Brd U阳性细胞数增多、Nestin平均光密度值增大,差异有显著性(P0.01);与患侧针刺组比较,巨刺组BrdU、Nestin阳性表达增加(P0.01)。结论:巨刺干预可以改善急性脑缺血再灌注损伤大鼠的神经功能,促进海马区神经干细胞的增殖,作为神经细胞再生的第一步,增殖的神经干细胞是具有分化为神经元、修复神经缺损症状的潜能,所以促进神经干细胞增殖的作用可能是巨刺治疗ICVD的作用机制之一,但其中的必然联系,需要进一步研究。
[Abstract]:Aim: to observe the effect of giant prick on the proliferation of neural stem cells in rats with acute cerebral ischemia reperfusion injury and to explore the mechanism of its effect on ischemic cerebrovascular disease. Methods: fifty SD rats were randomly divided into blank group (n = 10), sham-operated group (n = 10) and model group (n = 30). The rats in the model group were treated with modified longa thread embolization method to make the model of ischemia-reperfusion injury. After waking up, the rats were randomly divided into three groups: the model group, the affected side acupuncture group, and the giant prick group, each group with 10 rats in each group, and the rats were divided into three groups: the model group (n = 10) and the control group (n = 10). Blank group, sham-operation group, model group were not treated, and the affected side acupuncture group and giant prick group were treated with corresponding acupuncture and electroacupuncture intervention 24 hours after modeling, once a day for 7 times. At the end of the experiment, the nerve function defect was evaluated in each group of rats, and the morphological structure of cerebral cortex was observed under light microscope. The number of BrdU positive cells and the average optical density of nestin in the hippocampus of the ischemic side of rats were measured by immunohistochemistry and image analysis, so as to evaluate the proliferation of neural stem cells in rats with acute cerebral ischemia-reperfusion injury. The result is 1: 1. After the intervention, compared with the model group, the nerve function defect score of the affected side acupuncture group and the giant needling group decreased, and the difference was statistically significant compared with the affected side acupuncture group. There was no significant difference in the scores of the giant prickly group (P 0.05) and the difference between the two groups (P < 0.05). Brain morphology: compared with the model group and the affected side acupuncture group, the damage degree of brain tissue, the decrease of cell and perivascular space, and the increase of capillary were decreased in the giant prick group. Results of neural stem cell proliferation: compared with the blank group and sham operation group, the number of BrdU positive cells in the model group increased and the average optical density of nestin increased, the difference was statistically significant (P 0.01). The number of BrdU positive cells increased and the average optical density of nestin increased in the giant prickly group, the difference was significant (P 0.01), and the positive expression of BrdU nestin increased in the giant prick group as compared with that in the affected side acupuncture group. Conclusion: the intervention of giant spines can improve the neural function and promote the proliferation of neural stem cells in the hippocampus of rats with acute cerebral ischemia-reperfusion injury. As the first step of nerve cell regeneration, the proliferated neural stem cells are differentiated into neurons. The effect of nerve stem cells on the proliferation of neural stem cells may be one of the mechanisms of hyperbaric prickly therapy for iCVD, but the necessary link between them needs to be further studied.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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