电针对完全性脊髓损伤后神经源性膀胱大鼠脊髓组织中Caspase-9、细胞色素C及凋亡蛋白酶激活因子-1表达的影响

发布时间：2018-06-15 20:24

本文选题：脊髓损伤 + 神经源性膀胱　；参考：《中国康复理论与实践》2017年06期

【摘要】：目的观察电针对完全性脊髓损伤后神经源性膀胱大鼠膀胱功能及脊髓组织中Caspase-9、细胞色素C(Cyt-C)及凋亡蛋白酶激活因子-1(Apaf-1)表达的影响。方法雌性Sprague-Dawley大鼠60只,随机抽取36只,采用改良T_(10)脊髓横断法制作完全性脊髓损伤后神经源性膀胱模型,取24只成功模型分为模型组和电针组各12只,其余未造模大鼠24只随机分为假手术组和空白组各12只。于造模后第19天取次毼、中极、三阴交、大椎行电针,连续7 d后行尿流动力学检测,TUNEL法测定细胞凋亡率,Western blotting测定脊髓组织中Caspase-9、Cyt-C和Apaf-1蛋白的表达情况。结果与空白组和假手术组比较,模型组和电针组膀胱最大容量及膀胱顺应性均明显降低(P0.01),膀胱基础压力和漏尿点压力增加(P0.05);脊髓组织TUNEL阳性率显著升高(P0.001);脊髓组织中Caspase-9、Cyt-C和Apaf-1蛋白表达升高(P0.05)。与模型组比较,电针组的膀胱最大容量及膀胱顺应性明显增加(P0.01),膀胱基础压力和漏尿点压力降低(P0.05);脊髓组织TUNEL阳性率明显降低(P0.01);脊髓组织中Caspase-9、Cyt-C及Apaf-1蛋白表达量降低(P0.05)。结论电针次毼、中极、三阴交、大椎可改善完全性脊髓损伤后神经源性膀胱大鼠膀胱功能,其作用机制可能与脊髓组织中Caspase-9、Cyt-C和Apaf-1的表达下调,凋亡减少有关。
[Abstract]:Objective to observe the effects of electroacupuncture on bladder function and the expression of Caspase-9, cytochrome Cyt-C and apoptotic protease activator-1 Apaf-1 in spinal cord tissue of neurogenic bladder rats after complete spinal cord injury (sci). Methods Sixty female Sprague-Dawley rats were randomly selected and 36 rats were randomly selected. The neurogenic bladder model after complete spinal cord injury was made by modified T _ (10) spinal cord transection. 24 successful models were divided into two groups: model group (n = 12) and electroacupuncture group (n = 12). The other 24 rats were randomly divided into sham-operation group (n = 12) and blank group (n = 12). Electroacupuncture was performed on the 19th day after establishment of the model, followed by electroacupuncture at the middle pole, Sanyinjiao and Dazhui. The apoptosis rate was determined by Tunel method and the expression of Caspase-9 Cyt C and Apaf-1 protein in spinal cord tissue was determined by Western blotting. Results compared with the blank group and the sham operation group, The maximal bladder volume and bladder compliance in the model group and electroacupuncture group were significantly decreased, the basal pressure and leakage point pressure were increased (P 0.05), the positive rate of Tunel in spinal cord tissue was significantly increased (P 0.001), and the expression of Caspase-9 Cyt-C and Apaf-1 protein in spinal cord tissue was increased (P 0.05). Compared with the model group, the maximal bladder volume and bladder compliance in the electroacupuncture group were significantly increased, the basal bladder pressure and leakage point pressure were decreased (P 0.05), the positive rate of Tunel in spinal cord tissue was significantly decreased (P 0.01), and the expression of Caspase-9 Cyt-C and Apaf-1 protein was decreased in the spinal cord tissue (P 0.05). Conclusion electroacupuncture can improve the bladder function of neurogenic bladder rats after complete spinal cord injury. The mechanism may be related to the down-regulation of Caspase-9 Cyt-C and Apaf-1 expression and the decrease of apoptosis in spinal cord tissue.
【作者单位】：湖南中医药大学;
【分类号】：R245

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