督脉电针对慢性脑缺血大鼠OX2R及其配体表达的影响

发布时间：2018-06-16 20:12

本文选题：督脉电针 + 慢性脑缺血　；参考：《中医药学报》2017年01期

【摘要】：目的:研究督脉电针在慢性脑缺血时大鼠脑食欲素受体-2(OX2R)及其配体在不同时间点缺血后的变化。方法:实验中运用分次结扎双侧颈总动脉法建立慢性脑缺血模型,将Wistar大鼠分4组,造模成功后给予督脉电针干预,免疫组织化学法观察OX2R的表达,同时运用ELISA技术检测各组大鼠额叶皮质区Orexin-A和Orexin-B的总含量变化。结果:1个月、2个月时,模型组OX2R及其配体表达高于正常组,同时呈递增趋势;在相同时间进行组间比较,1个月时,模型组和针刺治疗组OX2R及其配体的表达无显著差别,立即针刺组OX2R及其配体的表达较模型组有升高;2个月时针刺治疗组OX2R及其配体表达较模型组有明显升高,而立即针刺组与针刺治疗组相比OX2R及其配体表达有升高。结论:实验观察到OX2R及其配体的表达在1个月和2个月时随着时间延长而升高,经过督脉电针干预后促进了OX2R及其配体表达,同时观察到早期干预对OX2R及其配体表达的影响优于针刺治疗组。
[Abstract]:Aim: to study the changes of orexin receptor-2 (OX2R) and its ligand after chronic cerebral ischemia in rats with du meridian electroacupuncture (EA) at different time points. Methods: the model of chronic cerebral ischemia was established by bilateral common carotid artery ligation. Wistar rats were divided into 4 groups. The rats were treated with du meridian electroacupuncture intervention and the expression of OX2R was observed by immunohistochemical method. The total contents of Orexin-A and Orexin-B in frontal cortex were detected by Elisa. Results: after 1 month and 2 months, the expression of OX2R and its ligand in model group was higher than that in normal group, and the expression of OX2R and its ligand in model group and acupuncture group had no significant difference at the same time. The expression of OX2R and its ligand in the immediate acupuncture group was higher than that in the model group, and the expression of OX2R and its ligand in the acupuncture group was significantly higher than that in the model group at 2 months, but the expression of OX2R and its ligand in the immediate acupuncture group was higher than that in the acupuncture group. Conclusion: it was observed that the expression of OX2R and its ligand increased with the prolongation of time at 1 and 2 months. The expression of OX2R and its ligand was promoted by du pulse electroacupuncture intervention. At the same time, the effect of early intervention on OX2R and its ligand expression was better than that of acupuncture group.
【作者单位】：黑龙江中医药大学;黑龙江中医药大学附属第一医院;
【基金】：黑龙江省教育厅科学技术研究项目(12541760)
【分类号】：R245.97

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