耳穴经皮电刺激对颞叶癫痫大鼠癫痫发作频率与海马区胶质细胞活性及炎性因子的影响

发布时间：2018-06-21 12:06

本文选题：颞叶癫痫 + 经皮耳穴电刺激　；参考：《针刺研究》2017年03期

【摘要】：目的:观察经皮电刺激耳迷走神经支配的耳穴对颞叶癫痫大鼠自发癫痫发作频率、海马区胶质细胞活性及促炎细胞因子白细胞介素(IL)-6、抗炎细胞因子IL-10表达的影响,探讨耳穴刺激对颞叶癫痫的缓解效应及其可能的细胞与分子机制。方法:SD大鼠随机分为正常组、模型组、治疗组,每组12只。采用氯化锂-匹罗卡品多次小剂量重复注射制备慢性颞叶癫痫大鼠模型。对治疗组耳甲腔内耳穴"心""肺""皮质下"进行经皮电刺激干预,每日1次,每次20min,治疗6周。用长时程视频系统监测大鼠的癫痫发作情况,治疗后用免疫荧光与Real-time PCR方法观察癫痫大鼠海马区小胶质细胞标记物Iba 1、星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)及IL-6、IL-10的表达情况。结果:模型组大鼠癫痫自发频率随时间延长显著增加,治疗组大鼠的自发癫痫频率较模型组显著降低(P0.05)。与正常组比较,模型组大鼠海马CA 1、CA 3区Iba 1、GFAP、IL-6免疫阳性细胞数明显增多(P0.05),IL-6mRNA表达水平显著上调(P0.05)。与模型组比较,治疗组大鼠海马CA 1、CA 3区Iba 1、GFAP、IL-6免疫阳性细胞数明显减少(P0.05),IL-6mRNA表达水平显著下调(P0.05),IL-10阳性细胞数及mRNA表达水平显著上调(P0.05)。结论:耳迷走神经支配的耳穴经皮电刺激干预能显著降低慢性颞叶癫痫大鼠的癫痫发作频率,此效应可能与下调海马区胶质细胞活性,下调海马内促炎细胞因子IL-6,上调抗炎细胞因子IL-10的蛋白及基因表达水平密切相关。
[Abstract]:Objective: to observe the effects of transcutaneous stimulation of auricular points innervated by auricular vagus on the frequency of spontaneous seizure, glial cell activity in hippocampus and the expression of interleukin-6, anti-inflammatory cytokine IL-10 in rats with temporal lobe epilepsy. To investigate the effect of auricular point stimulation on temporal lobe epilepsy and its possible cellular and molecular mechanisms. Methods: Sprague-Dawley rats were randomly divided into normal group, model group and treatment group with 12 rats in each group. The rat model of chronic temporal lobe epilepsy was established by repeated injection of lithium chloride and pilocarpine. The treatment group was treated with transcutaneous electrical stimulation of heart, lung and subcortical acupoints, once a day for 20 min, for 6 weeks. Long-term video system was used to monitor epileptic seizures in rats. The expressions of microglial markers Iba1, glial fibrillary acidic protein (GFAPs) and IL-6 IL-10 in hippocampal area of epileptic rats were observed by immunofluorescence and Real-time PCR after treatment. Results: the spontaneous frequency of epilepsy in the model group was significantly increased with the prolongation of time, and the frequency of spontaneous epilepsy in the treatment group was significantly lower than that in the model group. Compared with the normal group, the number of IL-6 immunoreactive cells in the CA1 / CA3 region of the model group was significantly increased. Compared with the model group, the number of IL-6 immunopositive cells in the CA1 / CA3 region of the hippocampus of the treatment group was significantly decreased. Conclusion: transcutaneous electrical stimulation of auricular points innervated by auricular vagus nerve can significantly decrease the frequency of epileptic seizures in rats with chronic temporal lobe epilepsy. This effect may be related to the down-regulation of glial cell activity in hippocampus. Down-regulation of IL-6 and up-regulation of protein and gene expression of anti-inflammatory cytokine IL-10 are closely related.
【作者单位】：中国中医科学院针灸研究所生物化学与分子生物学研究室;山东中医药大学针灸推拿学院;长春中医药大学针灸推拿学院;山东省中医药研究院;
【基金】：国家自然科学基金面上项目(No.81273833);国家自然科学基金青年基金项目(No.81303055) 国家重点基础研究发展计划“973”项目(No.2014CB 543103、2013CB 531904)
【分类号】：R245

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