耳针对脑缺血后睡眠剥夺大鼠下丘脑单胺类神经递质和细胞因子IL-1β的影响

发布时间：2018-06-22 08:29

本文选题：耳针 + 脑缺血后睡眠剥夺　；参考：《暨南大学学报(自然科学与医学版)》2017年03期

【摘要】：目的:通过观察耳针对脑缺血后睡眠剥夺大鼠下丘脑单胺类神经递质和细胞因子IL-1β的影响,探讨耳针治疗卒中后失眠的作用机制.方法:取SPF级Wistar雄性大鼠,用Zea Logna等报道的大脑中动脉栓线法(MCAO)制备大脑中动脉闭塞再灌注模型,模型成功后将动物随机分为对照组、模型组、安定组和耳针组4组,再对除对照组外的其他3组动物腹腔注射对氯苯丙氨酸(PCPA)造成脑缺血后睡眠剥夺大鼠模型,安定组每日腹腔注射安定,耳针组取耳穴神门(TF4)进行皮内针埋藏,均连续治疗7 d.采用酶联免疫吸附测定法(ELISA)检测下丘脑单胺类神经递质5-羟色胺(5-HT)、5-羟引哚乙酸(5-HIAA)、多巴胺(DA)、去甲肾上腺素(NE)和白介素-1β(IL-1β)的含量.结果:与对照组相比模型组大鼠下丘脑5-HT、5-HIAA和IL-1β含量减低(P0.05),DA、NE含量显著升高(P0.01);与模型组相比安定组与耳针组大鼠下丘脑5-HT和IL-1β含量显著升高(P0.01),DA、NE含量显著降低(P0.01).结论:耳针治疗卒中后失眠可能通过提高机体下丘脑5-HT、5-HIAA及细胞因子IL-1β含量,降低NE和DA的含量以调节和改善睡眠.
[Abstract]:Objective: to observe the effects of ear on monoamine neurotransmitters and cytokines IL-1 尾 in hypothalamus of rats with sleep deprivation after cerebral ischemia, and to explore the mechanism of auricular acupuncture on insomnia after stroke. Methods: the middle cerebral artery occlusion reperfusion model was established by Zea Logna method (MCAO) in SPF male Wistar rats. The rats were randomly divided into four groups: control group, diazepam group and ear acupuncture group. The model of sleep deprivation after cerebral ischemia was induced by intraperitoneal injection of p-chlorophenylalanine (PCPA) in the other three groups except the control group. Diazepam was injected intraperitoneally every day in the diazepam group, and the auricular acupoint Shenmen (TF4) in the auricular acupuncture group was implanted with intradermal needle for 7 days. The contents of 5-hydroxytryptamine (5-HT), 5-hydroxy3-3-acetic acid (5-HIAA), dopamine (DA), norepinephrine (NE) and interleukin-1 尾 (IL-1 尾) in the hypothalamus were determined by enzyme-linked immunosorbent assay (Elisa). Results: compared with the control group, the contents of 5-HT 5-HIAA and IL-1 尾 in the hypothalamus of the model group were significantly lower (P0.05) than those in the model group (P0.01), and the contents of 5-HT and IL-1 尾 in the hypothalamus of the diazepam group and the auricular acupuncture group were significantly higher (P0.01) than those in the model group (P0.01). Conclusion: ear acupuncture treatment of insomnia after stroke may regulate and improve sleep by increasing the contents of 5-HT 5-HIAA and cytokine IL-1 尾 in the hypothalamus and decreasing the contents of NE and DA.
【作者单位】：暨南大学中医学院;暨南大学附属第一医院针灸科;盘县中医院;深圳市南山区人民医院康复科;
【基金】：广东省科技计划项目(2014A020212221) 深圳市科技计划项目(JCYJ20140411094009916)
【分类号】：R245

【相似文献】