隔附子饼灸干预肠易激综合征大鼠脑肠互动轴的机理研究

发布时间：2018-06-22 23:12

本文选题：肠易激综合征 + 隔附子饼灸　；参考：《中华中医药学刊》2017年05期

【摘要】：目的:探讨隔附饼灸对肠易激综合征大鼠脑肠互动轴的干预机理。方法:本实验参照AL-chaer E D等的方法制备IBS-D大鼠模型,随机分成正常组、模型组、隔药饼灸组和匹维溴铵组,每组8只。采用不同处理方法后,进行AWR评分,检测各组结肠、脊髓和下丘脑的orexin蛋白的表达。结果:与正常组比较,模型组AWR评分明显升高,差异存在统计学意义(均P0.01),与模型组相比,隔药饼灸组和匹维溴铵组AWR评分明显降低,差异存在统计学意义(均P0.01);与正常组比较,模型组大鼠的结肠、下丘脑、脊髓Orexin A阳性目标总面积和积分光密度表达均明显降低,差异存在着统计学意义(均P0.05),与模型组比较,隔药饼灸组、匹维溴铵组大鼠的结肠、下丘脑、脊髓Orexin A阳性目标总面积和积分光密度表达表达明显增强,差异存在着统计学意义(均P0.05)。结论:隔药饼灸治疗IBS的机理可能是刺激T10-13的神经节段,经脑肠互动轴调节CNS系统、ENS系统增加orexin蛋白的表达,降低内脏高敏感而提高大鼠的痛阈。
[Abstract]:Objective: to investigate the interventional mechanism of cake-separated moxibustion on the interactive axis of brain and intestine in irritable bowel syndrome rats. Methods: the IBS-D rat model was established by referring to the method of AL-chaer E D et al. The rats were randomly divided into normal group, model group, moxibustion group and pivemine group, with 8 rats in each group. Awr score was used to detect the expression of orexin protein in colon, spinal cord and hypothalamus. Results: compared with the normal group, the AWR score in the model group was significantly higher than that in the control group (P0.01). Compared with the model group, the awr score in the moxibustion group and pilevermine group was significantly lower than that in the model group. Compared with the normal group, the total area and integral optical density of the positive target of Orexin A in the colon, hypothalamus and spinal cord in the model group were significantly lower than those in the model group (P0.05). The total area and integral optical density expression of orexin A in colon, hypothalamus and spinal cord of rats in moxibustion group and piveronium bromide group were significantly increased (P0.05). Conclusion: the mechanism of moxibustion with medicine separated cake in treating IBS may be to stimulate the nerve segment of T10-13, regulate the expression of orexin protein in the CNS system and increase the pain threshold of rats by regulating the expression of orexin protein and decreasing visceral hypersensitivity through the interactive axis of brain and intestine.
【作者单位】：江西中医药大学基础医学院;江西中医药大学研究生院;湖南岳阳天添药用胶囊有限公司;上海市针灸经络研究所;江西中医药大学人文学院;
【基金】：江西省自然科学基金项目(20114BAB205084)
【分类号】：R245

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