电针预处理对MCAO大鼠模型血脑屏障和MMP-9的影响

发布时间：2018-06-25 00:58

本文选题：电针预处理 + 缺血再灌注损伤　；参考：《神经解剖学杂志》2017年04期

【摘要】：目的:探讨电针(EA)预处理对大鼠脑缺血再灌注后血脑屏障(BBB)通透性及脑内基质金属蛋白酶-9(MMP-9)表达的影响。方法:96只成年雄性SD大鼠随机分为假手术(Sham)组、单纯电针(EA)组、缺血(MCAO)组及电针预处理(EA+MCAO)组,每组24只。使用线栓法制作大鼠大脑中动脉阻塞(MCAO)模型,缺血120 min再灌注48 h后处死取脑。分别采用干湿重法测定各组动物脑水含量,伊文斯蓝(EB)法检测血脑屏障通透性,Western Blot检测紧密连接蛋白(TJP)及MMP-9表达水平,同时明胶酶谱法检测MMP-9活性。结果:与Sham组相比,MCAO组大鼠脑水含量及EB含量增多(P0.05),缺血侧脑组织TJP水平下降(P0.05),MMP-9表达及活性明显升高(P0.05);与MCAO组大鼠比较,EA+MCAO组脑水含量及EB含量减少(P0.05),缺血侧脑组织TJP水平升高(P0.05),同时MMP-9表达及活性显著降低(P0.05)。结论:电针预处理通过抑制缺血后脑内MMP-9的表达及活性,维持BBB完整性,有效改善脑缺血后的脑水肿症状。
[Abstract]:Aim: to investigate the effect of electroacupuncture (EA) preconditioning on the permeability of blood-brain barrier (BBB) and the expression of matrix metalloproteinase-9 (MMP-9) after cerebral ischemia-reperfusion in rats. Methods Ninety-six adult male Sprague-Dawley rats were randomly divided into sham-operated (Sham) group, electroacupuncture (EA) group, ischemia (MCAO) group and EA MCAO group with 24 rats in each group. The rat model of middle cerebral artery occlusion (MCAO) was established by the method of thread occlusion. The rats were killed after 120 min of ischemia and 48 h of reperfusion. The contents of brain water in each group were measured by dry and wet weight method. The expression of tight junction protein (TJP) and MMP-9 were detected by Western blot with Evans blue (EB) method. The activity of MMP-9 was detected by gelatin zymogram. Results: compared with the Sham group, the contents of brain water and EB in the MCAO group increased (P0.05), the expression and activity of MMP-9 in ischemic brain tissue decreased (P0.05), the content of brain water and EB in the MCAO group decreased (P0.05), and the content of EB in the ischemic side of the brain decreased (P0.05). Tissue TJP level increased (P0.05), while MMP-9 expression and activity decreased significantly (P0.05). Conclusion: electroacupuncture preconditioning can effectively improve the symptoms of cerebral edema after cerebral ischemia by inhibiting the expression and activity of MMP-9 and maintaining the integrity of BBB.
【作者单位】：第四军医大学西京医院麻醉科;第四军医大学西京医院心脏内科;
【基金】：国家自然科学基金(81370194)
【分类号】：R245;R-332

【相似文献】