MiR-193b介导的电针减少Stat5NKO肥胖小鼠体重的机制研究

发布时间：2018-06-27 14:36

本文选题：电针 + 肥胖　；参考：《南京中医药大学》2016年硕士论文

【摘要】：目的：观察电针对Stat5NK0小鼠的减肥效应及与miR-193b的相关性。方法：选择周龄在3-4周的Stat5fl/fi 和 Statffl/fl;Nestin-cre (StatfNKO)雄性小鼠,所有小鼠均喂食普通标准饲料,记录体重,以体重大于等于Stat5fl/fl组小鼠平均体重20%的标准入选SSttat5NKO组。再把Stat5ffl/fl和Stat5NKO小鼠随机分为对照组和针刺组(Stat5fl/fl+EA;Stat5NO+EA)。使用特殊工具将小鼠固定,并在小鼠清醒状态下进行电针干预,电针穴取小鼠“后三里”和“内庭”,对照组只捆不针。每周记录小鼠体重。在电针治疗前后,分别对小鼠进行糖耐量实验(IGTT)。电针治疗结束后,进行冷库实验。处死小鼠,取血清检测瘦素、胰岛素、甘油三酯、胆固醇和低密度脂蛋白；取小鼠附睾脂肪并称重。使用苏木精一伊红染色法(hematoxylin-eosinstaining、HE Staining)研究针刺对脂肪组织的作用。使用实时荧光定量核酸扩增检测系统(real-time quantitative PCR detecting system, qPCR)检测miR-193b在小鼠不同组织中的表达差异,检测解耦联蛋白(UCP1)在骨骼肌与附睾脂肪中的表达水平。结果：电针有效降低了肥胖小鼠的体重,体脂比显著低于对照组,电针组小鼠糖耐量有一定程度改善,血脂生化指标显著改善,耐寒性得到提高。HE染色可见电针组小鼠附睾脂肪细胞体积减小。qPCR实验证实,miR-193b在小鼠的棕色脂肪中含量最为丰富；Stat5NKO+EA组小鼠外周组织中miR-193b的表达下降,而在中枢表达上升；电针能促进UCP1在骨骼肌和附睾脂肪中的表达。结论：电针能降低5Stat5NKO肥胖小鼠的体重；miR-193b在小鼠棕色脂肪中表达最为丰富；电针能调控miR-193b在组织中的表达,这可能需要中枢Stat5的参与。
[Abstract]:Objective: to observe the effect of electroacupuncture on the weight loss of Stat5NK0 mice and its correlation with miR-193b. Methods: Stat5fl-fi and Statflt-nestin-cre (StatfNKO) male mice aged 3-4 weeks were selected. All the mice were fed with normal diet and their body weight was recorded. The average body weight of SSttat5NKO group was 20% or more than that of Stat5fl-fl group. Stat5ffl / fl and Stat5NKO mice were randomly divided into control group and acupuncture group. The mice were fixed with special tools, and electroacupuncture intervention was carried out in the awake state of the mice. The mice were taken from the electroacupuncture points for "three li" and "chambers", while the control group was not tied with the needles. The weight of mice was recorded weekly. Glucose tolerance test (IGTT) was performed on mice before and after electroacupuncture treatment. After electroacupuncture treatment, cold storage experiment was carried out. Serum leptin, insulin, triglyceride, cholesterol and low density lipoprotein were measured, and epididymal fat was taken and weighed. The effect of acupuncture on adipose tissue was studied by hematoxylin-eosin stenting (HE Staining). Real-time quantitative detecting system (qPCR) was used to detect the expression of miR-193b in different tissues of mice and the expression level of UCP1 in skeletal muscle and epididymal fat. Results: electroacupuncture effectively reduced the body weight of obese mice, the body fat ratio was significantly lower than that of the control group, the glucose tolerance of the mice in the electroacupuncture group was improved to a certain extent, and the biochemical indexes of blood lipids were significantly improved. The enhanced cold tolerance. He staining showed that the volume of epididymal adipocytes in electroacupuncture group was reduced. QPCR results showed that miR-193b was the most abundant in brown fat of mice. The expression of miR-193b in peripheral tissues of mice in Stat5NKO EA group decreased, but increased in the center of mice. Electroacupuncture can promote the expression of UCP1 in skeletal muscle and epididymal fat. Conclusion: electroacupuncture can reduce the expression of miR-19b in brown fat of 5Stat5NKO obese mice, and electroacupuncture can regulate the expression of miR-193b in tissues, which may require the participation of central Stat5.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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