针刺对卒中后抑郁大鼠脑组织神经递质基因表达影响

发布时间：2018-07-08 18:25

本文选题：卒中后抑郁 + 针刺　；参考：《中国针灸》2017年06期

【摘要】：目的:观察针刺对卒中后抑郁大鼠脑组织去甲肾上腺素神经递质五羟色胺(5-HT)转运体(5-HTT)、5-HT_(1A)受体(5-HT_(1A) R)、去甲肾上腺素α_2受体(NEα_2R)m RNA的影响。方法:将40只雄性SD大鼠随机分为正常组、模型组、药物组、针刺组,每组各10只。采用大脑中动脉闭塞和慢性不可预见性温和性应激法复合制备卒中后抑郁模型。造模成功后,正常组、模型组不予干预,药物组予以氟西汀(2 mg/kg)灌胃,针刺组选取"百会""风府""神门""太冲"穴,针刺20 min,期间行针1次,每日1次,7 d为一疗程,共3个疗程,疗程之间休息1 d。观察干预后各组大鼠行为学及5-HT、NE变化,采用逆转录聚合酶链式反应(RT-PCR)观察海马、中缝核、蓝斑核5-HTT、5-HT_(1A) R、NEα_2R m RNA变化。结果:治疗后,与正常组比较,模型组Zea Longa评分升高,糖水消耗量、敞箱水平运动、敞箱垂直运动次数降低(均P0.01);与模型组比较,针刺组、药物组Zea Longa评分降低,糖水消耗量、敞箱水平运动、敞箱垂直运动次数升高(P0.01,P0.05);针刺组和药物组比较,大鼠行为学变化无统计学意义(均P0.05)。治疗后,与正常组比较,模型组脑组织5-HT、NE及海马、中缝核、蓝斑核5-HTT m RNA、5-HT_(1A) R m RNA表达降低(均P0.01),NEα_2R m RNA升高(P0.01);与模型组比较,针刺组、药物组脑皮质5-HT、NE含量明显升高(均P0.01),海马、中缝核、蓝斑核5-HTT、5-HT_(1A) R mRNA表达升高(P0.01,P0.05),NEα_2R m RNA降低(均P0.01);针刺组与药物组比较差异无统计学意义(均P0.05)。结论:针刺能明显改善卒中后抑郁大鼠行为学变化,其机制可能与调节大鼠脑皮质5-HT、NE及海马、中缝核、蓝斑核5-HTT、5-HT_(1A)R、NEα_2R mRNA表达有关。
[Abstract]:Aim: to observe the effect of acupuncture on norepinephrine neurotransmitter serotonin (5-HT) transporter (5-HTT) 5-HT _ (1A) receptor (5-HT _ (1A) R) and norepinephrine 伪 _ 2 receptor (NE 伪 2R) mRNA in the brain of poststroke depression rats. Methods: forty male SD rats were randomly divided into normal group, model group, drug group and acupuncture group with 10 rats in each group. Middle cerebral artery occlusion (MCAO) and chronic unpredictable mild stress were used to establish poststroke depression model. After the model was successfully established, the normal group and the model group were given no intervention. The drug group was given fluoxetine (2 mg/kg) intragastrically, and the acupuncture group selected "Baihui" Fengfu "Shenmen" Taochong "point, acupuncture for 20 minutes, during which acupuncture was given once, once a day for 7 days as a course of treatment. A total of 3 courses of treatment, between courses of rest 1 day. The changes of behavior and 5-HT NE were observed after intervention. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to observe the changes of 5-HTT1-5-HT1A 伪 2R mRNA in hippocampus, raphe nucleus and nucleus coeruleus. Results: compared with normal group, Zea Longa score increased, sugar water consumption, open box horizontal exercise, open box vertical movement decreased in model group (P0.01), Zea Longa score decreased and sugar water consumption decreased in acupuncture group and drug group, compared with model group. Open box horizontal exercise, open box vertical movement times increased (P0.01P05); acupuncture group and drug group, rats behavior changes were not statistically significant (P0.05). After treatment, the expression of 5-HTTNE in brain tissue and 5-HTT _ (1A) R mRNA expression in hippocampus, raphe nucleus and locus coeruleus in model group was significantly lower than that in normal group (P0.01). In raphe nucleus and locus coeruleus, the expression of 5-HTT _ (1A) R mRNA increased (P0.01P 0.05) and NE 伪 -2R mRNA decreased (P0.01), but there was no significant difference between acupuncture group and drug group (P0.05). Conclusion: acupuncture can obviously improve the behavioral changes in rats with post-stroke depression, and its mechanism may be related to the regulation of the expression of 5-HTT1-NE, hippocampal, raphe nucleus and locus coeruleus 5-HT _ (1A) RNE 伪 2R mRNA in rat cerebral cortex.
【作者单位】：安徽中医药大学第二附属医院;安徽中医药大学;
【基金】：国家自然科学基金面上项目:81273857
【分类号】：R245

【相似文献】