不同炮制方法对蒙古族药狼毒毒效的影响

发布时间：2018-07-09 19:53

  本文选题：蒙古族药 + 狼毒　； 参考：《中国实验方剂学杂志》2017年22期

【摘要】：目的:探讨蒙古族药狼毒炮制减毒药性理论的科学性,了解其炮制前后药效变化,并建立各样品中岩大戟内酯B和狼毒乙素含量测定的RP-HPLC。方法:采用HPLC-Q-TOF/MS技术比较蒙古族药狼毒炮制前后的成分差异;通过正常小鼠的急性毒性作用及泻下作用比较炮制对该药材毒性和药效的影响,利用RP-HPLC测定蒙古族药狼毒及其炮制品中岩大戟内酯B和狼毒乙素的含量,流动相乙腈(A)-0.1%冰乙酸水溶液(B)梯度洗脱(0~5 min,40%A;5~10 min,40%~50%A;10~30 min,50%~55%A;30~40 min,55%~60%A;40~45 min,60%~70%A),检测波长250 nm,柱温30℃。结果:与生品相比,蒙古族药狼毒在炮制后奶制品、诃子汤制品、酒制品中分别有30,20,21个峰的含量降低。狼毒生品及奶、酒、诃子汤制品粉末的半数致死量分别为2.694,3.642,3.461,4.309 g·kg~(-1)。药效试验表明药物粉末给药剂量为0.53,0.133 g·kg~(-1)时,狼毒生品及其各炮制品均能明显提高小鼠小肠墨汁推进率。狼毒中狼毒乙素和岩大戟内酯B的线性范围分别为0.060 12~1.202 4,0.060 72~1.214 4μg,平均加样回收率分别为107.55%(RSD 4.7%)和103.03%(RSD 3.6%)。结论:蒙古族药狼毒不同方法炮制后其化学成分含量会不同程度降低,进而可能会降低部分刺激性成分含量或使其转化为毒性较小成分,从而降低了其毒性。蒙古族药狼毒炮制后的粉末较未经炮制的粉末毒性降低,炮制后对该药材的泻下作用无影响,即炮制可减毒保效。狼毒炮制机制可能与炮制后化学成分含量变化有关。建立的HPLC准确可靠、重复性好,可用于蒙古族药狼毒及其炮制品中岩大戟内B和狼毒乙素的含量测定。
[Abstract]:Objective: To explore the scientificalness of the theory of the drug resistance of Mongolian medicine and to understand the changes of the drug effect before and after its processing, and to establish a RP-HPLC. method for the determination of the content of euphorbiate B and the content of schyomo B in each sample: To compare the difference of the composition of the Mongolian medicine by HPLC-Q-TOF/MS and the acute toxicity of the normal mice. The effects of the artillery on the toxicity and efficacy of the medicinal materials were compared and the content of Euphorbia euphorbialis B and schyomo ethyl in the Mongolian medicine wolf poison and its processed products were measured by RP-HPLC, and the gradient elution of the mobile phase acetonitrile (A) -0.1% glacial acetic acid solution (B) (0~5 min, 40%A; 5~10 min, 40%~50%A; 10~30) 60%~70%A), the detection wavelength was 250 nm, and the column temperature was 30. Results: compared with the raw products, the content of 30,20,21 peaks in the Mongolian medicine wolf poison was lower than that of the milk products, and the half lethal amount of the powder of the raw and milk, wine, and sijo soup products were divided into 2.694,3.642,3.461,4.309 G. Kg~ (-1). When the amount of powder was given to 0.53,0.133 G. Kg~ (-1), the growth rate of murine small intestine ink could be improved obviously. The linear range of 0.060 12~1.202 4,0.060 72~1.214 4 g, respectively, was 107.55% (RSD 4.7%) and 103.03% (RSD 3.6%) respectively. The chemical composition of Mongolian medicine wolf poison can be reduced in varying degrees, and then it may reduce the content of part of the stimulant component or make it into less toxic component, thus reducing its toxicity. The toxicity of the powder produced by Mongolian medicine wolf poison is lower than that of the unprocessed powder. The mechanism of the artillery preparation may be related to the change of chemical composition after the processing. The established HPLC is accurate, reliable and reproducible, and can be used for the determination of the content of B in the Euphorbia Mongolia and its artillery products.
【作者单位】： 江西中医药大学;内蒙古民族大学;
【基金】：内蒙古自治区蒙医药科技重大专项(GCY20161219)
【分类号】：R29
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本文编号：2110480