藏药印度獐牙菜抗四氯化碳诱导的大鼠肝纤维化的作用

发布时间：2018-07-09 21:23

本文选题：藏药 + 印度獐牙菜　；参考：《中华中医药杂志》2017年10期

【摘要】：目的:观察印度獐牙菜对四氯化碳(CCl4)所致大鼠肝纤维化的防治作用。方法:32只SD大鼠随机分为空白组、模型组、印度獐牙菜组、阳性药组。除空白组外,各组大鼠腹腔注射40%CCl4/橄榄油溶液2m L/kg,每周2次,连续7周诱导肝纤维化。造模同时,给药组大鼠灌胃相应药物干预8周。实验结束后,观察肝组织病理学变化;测定血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原蛋白(ColⅣ)水平以及丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)含量。结果:与空白组比较,模型组可见明显肝纤维化病理改变,AST、ALT、ALP和TBIL水平明显升高(P0.05),HA、LN、PCⅢ和ColⅣ含量显著增高(P0.01);与模型组比较,印度獐牙菜组肝纤维化病理程度减轻,肝功能指标改善明显,ALT、TBIL水平显著降低(P0.05),AST、ALP水平呈下降趋势,HA、LN、PC及ColⅣ含量显著下降(P0.01)。结论:印度獐牙菜具有较好的抗肝纤维化作用。
[Abstract]:Objective: To observe the effect of India Swertia on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats. Methods: 32 SD rats were randomly divided into blank group, model group, India Swertia group, positive drug group. Except for the blank group, the rats were intraperitoneally injected with 40%CCl4/ olive oil 2m L/kg, and the liver fibrosis was induced for 7 weeks in a week. The rats in the drug group were treated with corresponding drug intervention for 8 weeks. After the experiment, the pathological changes of liver tissues were observed. Serum hyaluronic acid (HA), laminin (LN), type III procollagen (PC III) and type IV collagen (Col IV), alanine aminotransferase (AST), ALP (ALP), total bilirubin, and total bilirubin were measured. (TBIL) content. Results: compared with the blank group, the pathological changes of liver fibrosis were obvious in the model group, and the levels of AST, ALT, ALP and TBIL increased significantly (P0.05), HA, LN, PC III and Col IV increased significantly (P0.01). Compared with the model group, the pathological degree of liver fibrosis in India Swertia group was reduced, the liver function index was obviously improved, ALT, levels decreased significantly. 0.05) the level of AST and ALP showed a downward trend, while the contents of HA, LN, PC and Col IV decreased significantly (P0.01). Conclusion: Swertia India has a good anti hepatic fibrosis effect.
【作者单位】：南京中医药大学附属中西医结合医院;江苏省中医药研究院;
【基金】：江苏省药学会奥赛康临床药学研究基金项目(No.210303)~~
【分类号】：R29

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