基于脾虚肝癌小鼠Oatp2b1的表达探讨“因湿致瘀”的内涵

发布时间：2018-07-31 15:24

【摘要】：目的通过观察脾虚肝癌小鼠的各组织有机阴离子转运肽2b1表达的情况探讨脾虚肝癌"因湿致瘀"的机制。方法C57BL/6小鼠随机分为空白组、脾虚组、肝癌组和脾虚肝癌组。观察肝癌小鼠的体质量和瘤体大小,并取各组小鼠肺、胃、肝、肾、脾、结肠、小肠和肝癌组织,检测各组织中Oatp2b1表达情况。结果脾虚肝癌组与肝癌组小鼠的体质量和瘤体体积出现差异(P0.05)。Oatp2b1 mRNA在脾虚肝癌小鼠的肺、脾、结肠和小肠组织表达量较肝癌组升高(P0.05),在癌组织中表达量也明显升高(P0.05),而肝组织中表达却明显降低(P0.05)。结论 Oatp2b1在脾虚肝癌小鼠肝组织的低表达和癌组织高表达,可能是脾虚肝癌"因湿致瘀"的分子机制之一。
[Abstract]:Objective to observe the expression of organic anion transporter peptide (2b1) in various tissues of mice with spleen deficiency liver cancer and to explore the mechanism of liver cancer due to dampness and blood stasis. Methods C57BL/6 mice were randomly divided into blank group, spleen deficiency group, liver cancer group and spleen deficiency liver cancer group. The body weight and tumor size of hepatoma mice were observed, and the expression of Oatp2b1 was detected in the lung, stomach, liver, kidney, spleen, colon, small intestine and liver cancer tissues of each group. Results there was significant difference in body weight and tumor volume between liver cancer group and liver cancer group (P0.05). Oatp2b1 mRNA was found in lung and spleen of liver cancer mice with spleen deficiency. The expression of colon and small intestine was significantly higher than that of liver cancer (P0.05), but the expression of liver was significantly decreased (P0.05). Conclusion the low expression of Oatp2b1 and the high expression of Oatp2b1 in liver tissue of mice with spleen deficiency may be one of the molecular mechanisms of liver cancer caused by dampness and stasis.
【作者单位】：中山大学附属第三医院国家中医肝病重点专科广东省肝脏疾病研究重点实验室;中山大学附属第一医院;
【基金】：国家自然科学基金面上项目(No.81072806;No.81373500) 广东省自然科学基金(No.S2012010009277)
【分类号】：R273

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