基于一测多评法研究畲药食凉茶黄酮类物质动态变化规律

发布时间：2018-08-02 12:20

【摘要】：目的一测多评(QAMS)法测定畲药食凉茶中4种黄酮类成分,并以其研究食凉茶黄酮类物质的动态变化规律。方法以芦丁为参照物,分别建立其与山柰酚-3-O-芸香糖苷、槲皮素、山柰素的相对校正因子(f),并计算食凉茶中各成分量,实现QAMS。同时采用外标法验证QAMS法的准确性和可行性。结果芦丁与山柰酚-3-O-芸香糖苷、槲皮素和山柰素的f分别为1.158、0.475 6、0.431 9,RSD分别为0.73%、1.55%、1.58%;QAMS法测定结果与外标法测定结果无显著差异。不同月份食凉茶中黄酮类成分质量分数差异明显,2个来源食凉茶之间的量差异亦较明显。结论以芦丁为参照物同时测定山柰酚-3-O-芸香糖苷、槲皮素、山柰素的QAMS法可用于畲药食凉茶的定量分析。以黄酮类物质为指标,食凉茶的最佳采收期为7月和8月。
[Abstract]:Objective to determine four kinds of flavonoids in she herbal tea by (QAMS), and to study the dynamic changes of flavonoids in traditional Chinese herbal tea. Methods the relative correction factors (f), of rutin and kaempferol 3-O- rutin, quercetin and kaempferol were established, and the contents of components in herbal tea were calculated to realize QAMS. At the same time, the accuracy and feasibility of QAMS method were verified by external standard method. Results the f of rutin and kaempferol 3-O- rutin, quercetin and kaempferol were 1.158, 0.475, 6, 0.431 9, respectively. The RSD of Rutin, kaempferol and kaempferol were 0.73 and 1.551.58, respectively. There was no significant difference between Rutin and kaempferol by QAMS method and external standard method. The contents of flavonoids in Chinese herbal tea were significantly different in different months, and the difference in quantity between two sources of herbal tea was also obvious. Conclusion the quantitative analysis of kaempferol 3-O- rutin, quercetin and kaempferol with rutin as reference material can be used for the quantitative analysis of herbal tea. The optimum harvest time of herbal tea was July and August with flavonoids as index.
【作者单位】：丽水市食品药品与质量技术检验检测院;
【基金】：丽水市高层次人才培养项目(2015RC25) 浙江省食品药品监督管理与产业发展研究会项目(ZYH2016013)
【分类号】：R29

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