维药昆仑雪菊多糖对四氯化碳所致小鼠急性肝损伤的预防作用及机制研究

发布时间：2018-08-03 12:55

【摘要】：目的:研究维药昆仑雪菊多糖(KSCP)对四氯化碳(CCl_4)所致小鼠急性肝损伤的预防作用及机制。方法:将96只小鼠随机分为正常组(生理盐水)、模型组(生理盐水)、联苯双酯滴丸组(阳性对照,1.5 mg/10 g)和KSCP低、中、高剂量组(0.3、0.6、1.2 mg/10 g),每组16只,ig给药,每天1次,连续10 d。除正常组外,其余各组小鼠均ip 1%CCl_4菜籽油溶液诱导肝损伤。造模24 h后,检测各组小鼠血清中谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)的水平和肝组织中丙二醛(MDA)、超氧化物歧化物(SOD)的水平,计算小鼠肝、脾指数,观察肝组织病理变化并进行病理评分,检测肝组织中凋亡相关基因Caspase-3、Bcl-2、Bax蛋白表达。结果:与正常组比较,模型组小鼠血清中ALT、AST、TNF-α、IL-1水平和肝组织中MDA水平以及肝、肾指数均明显升高(P0.01),肝组织中SOD水平明显降低(P0.01);肝组织发生肝细胞坏死、变性和炎症细胞浸润等病理变化,病理评分明显增加(P0.01);肝组织中Caspase-3蛋白表达水平以及Bcl-2/Bax比值均明显降低(P0.01)。与模型组比较,各给药组小鼠上述指标均明显改善(P0.05或P0.01)。结论:KSCP对CCl_4所致小鼠急性肝损伤有一定预防作用,其机制可能与抗氧化、抗炎及调节凋亡相关蛋白的表达有关。
[Abstract]:Aim: to study the preventive effect and mechanism of (KSCP) on acute liver injury induced by carbon tetrachloride (CCl_4) in mice. Methods: 96 mice were randomly divided into normal group (normal saline), model group (normal saline), biphenyl diester dropping pill group (positive control group, 1.5 mg/10 g) and low KSCP. Liver injury was induced by IP 1l_4 rapeseed oil solution in all mice except the normal group. The serum levels of glutamate aminotransferase (ALT), aspartate transaminase (AST), tumor necrosis factor 伪 (TNF- 伪), interleukin-1 (IL-1) and malondialdehyde (MDA),) superoxide dismutates (SOD) in liver tissue were measured after 24 h model making. The liver and spleen indexes were calculated. The pathological changes of liver tissue were observed and the expression of apoptosis-related gene Caspase-3 Bcl-2mBax protein was detected. Results: compared with the normal group, the levels of alt ASTNF- 伪 -1 in serum, MDA level in liver tissue, liver and kidney index in model group were significantly increased (P0.01), the level of SOD in liver tissue was significantly decreased (P0.01), hepatocyte necrosis occurred in liver tissue. Pathological changes, such as degeneration and inflammatory cell infiltration, significantly increased the pathological score (P0.01), and decreased the expression of Caspase-3 protein and the ratio of Bcl-2/Bax in liver tissue (P0.01). Compared with the model group, the above indexes were significantly improved in each administration group (P0.05 or P0.01). Conclusion KSCP can prevent acute liver injury induced by CCl_4 in mice, and its mechanism may be related to anti-oxidation, anti-inflammation and regulating the expression of apoptosis-related proteins.
【作者单位】：新疆医科大学中心实验室;石河子大学药学院;
【基金】：国家自然科学基金资助项目(No.81560680)
【分类号】：R29

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