埋线预处理对缺血再灌注损伤ZDF大鼠心肌ATG9A及p62蛋白表达的影响

发布时间：2018-08-12 07:55

【摘要】：目的:以自噬相关蛋白9A(ATG9A)及p62为关键点,探讨埋线预处理防治心肌缺血再灌注损伤的作用机制。方法:ZDF大鼠随机分为:正常组、模型组、缺血预处理组和埋线预处理组。以推管法制备缺血再灌注损伤模型。电镜观察心肌组织超微结构;测定乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)的活性变化;Western blot法检测ATG9A及p62蛋白表达的水平。结果:电镜下,缺血预处理组及埋线预处理组自噬泡数量较模型组明显增多,心肌超微结构损伤较轻;与模型组比较,缺血预处理组和埋线预处理组血清LDH、CK-MB值均明显下降(P0.01),心肌ATG9A蛋白表达显著上升(P0.01),p62蛋白水平下降(P0.01)。结论:埋线预处理可能通过上调心肌中ATG9A、下调p62的表达水平激活自噬,从而有效对抗缺血再灌注损伤。
[Abstract]:Aim: to investigate the mechanism of preconditioning with embedding thread to prevent and treat myocardial ischemia-reperfusion injury with autophagy associated protein 9A (ATG9A) and p62 as the key points. Methods: ZDF rats were randomly divided into normal group, model group, ischemic preconditioning group and embedding preconditioning group. The model of ischemia-reperfusion injury was established by pushing tube. The ultrastructure of myocardium was observed by electron microscope, and the activities of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) were measured. The expression of ATG9A and p62 protein was detected by Western blot. Results: under electron microscope, the number of autophagy in ischemic preconditioning group and embedding preconditioning group was significantly higher than that in model group, and the myocardial ultrastructure damage was less in ischemic preconditioning group than in model group. In the ischemic preconditioning group and the catgut preconditioning group, the level of serum LDH- CK-MB was significantly decreased (P0.01), and the expression of myocardial ATG9A protein increased significantly (P0.01), and the level of p62 protein decreased (P0.01). Conclusion: catgut implantation preconditioning may activate autophagy by up-regulating ATG9A and down-regulating the expression of p62, thus effectively antagonizing ischemia-reperfusion injury.
【作者单位】：江苏大学附属医院针灸科;黑龙江中医药大学附属第二医院重症医学科;哈尔滨医科大学附属第二医院;
【基金】：国家自然科学基金项目(No.81303037)~~
【分类号】：R245

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