半导体激光光灸“足三里”对急性佐剂关节炎大鼠的镇痛效应研究

发布时间：2018-08-12 12:05

【摘要】：目的:采用波长为830nm半导体激光器照射急性佐剂性关节炎大鼠"足三里"穴,观察穴位处肥大细胞脱颗粒情况、足跖周长和痛阈值等的变化,从而研究激光照射后大鼠"足三里"穴对大鼠关节炎的镇痛效果。方法:清洁级Wistar大鼠45只,随机分为空白对照组、模型组、光灸组各15只,其中空白对照组未做任何处理,模型组在造模之后不进行治疗,光灸组大鼠在造模后第1天开始对其用激光器照射右后腿的"足三里"穴位20min。应用辐射热缩爪反射的方法检测大鼠的痛阈值,且对其足跖周长、爪大小及穴位组织肥大细胞脱颗粒情况进行分析。结果:空白对照组的右侧后爪的大小一直未变,但是光灸组在治疗第4个疗程后变化非常显著;而且发现光灸组在造模后第29天与造模后第1天相比,足跖周长差异显著,但是模型组和空白对照组未见显著差异,组织切片图发现光灸组穴位处肥大细胞脱颗粒现象明显;另外对比造模后第29天各组之间的痛阈差异,发现空白对照组和光灸组与模型组相比有显著差异。结论:半导体激光光灸大鼠"足三里"具有镇痛、消炎的效果。
[Abstract]:Objective: to observe the degranulation of mast cells at acupoints, the circumference of foot plantar and the threshold of pain in acute adjuvant arthritis rats irradiated with 830nm semiconductor laser at wavelength. To study the analgesic effect of Zusanli (Zusanli) on arthritis of rats after laser irradiation. Methods: Forty-five clean grade Wistar rats were randomly divided into control group, model group and light moxibustion group. The rats in the light moxibustion group began to irradiate the "Zusanli" acupoint of the right hind leg with laser for 20 min on the first day after the model was made. The pain threshold of rats was detected by the method of radiation heat shrinking claw reflex, and the peripheral length of foot plantar, the size of paw and degranulation of mast cells in acupoint tissue were analyzed. Results: the size of the right hind paw in the blank control group remained unchanged, but the light moxibustion group had a significant change after the fourth course of treatment, and it was found that the circumference of the foot and metatarsus in the light moxibustion group was significantly different from that in the model group on the 29th day and the first day after the model making. However, there was no significant difference between the model group and the blank control group, and the tissue sections showed that the degranulation of mast cells at the acupoints in the light moxibustion group was obvious. In addition, the pain threshold of each group on the 29th day after modeling was compared. It was found that there were significant differences between the blank control group and the light moxibustion group and the model group. Conclusion: semiconductor laser moxibustion of Zusanli has analgesic and anti-inflammatory effects.
【作者单位】：华南师范大学生物光子学研究院国家中医药管理局中医药与光子技术三级实验室;无限极(中国)有限公司;
【基金】：无限极(中国)有限公司委托重大技术开发项目(HPG/2013/11/1598) 国家自然科学基金项目(31300691;61275187;61335011;11404116) 教育部高等学校博士学科点专项科研基金项目(20114407110001;20134407120003) 广东省自然科学基金重点项目(9251063101000009) 广东省重大科技专项项目(2012A080203008) 广东省教育厅科技创新项目(2013KJCX0052)
【分类号】：R245

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