针刺干预SD大鼠排卵的机理探究

发布时间：2018-08-13 13:04

【摘要】：目的:探讨针刺促进卵泡生长、发育、成熟、排出的效应机理及对生殖内分泌激素的影响,阐释传统中医疗法的科学内涵,为临床开展针刺促排卵提供科学依据。方法:将27只具有正常发情周期的大鼠,按照体重随机分成空白组、针刺组、安慰针组3组,每组9只。针刺组选取"中极"、"气海"、"百会"、双侧"三阴交"、双侧"合谷",电针连接"中极"与"气海";安慰针组选取未知穴位,双侧股骨大转子与后海(尾根与肛门之间的凹陷处)中点,双侧肩峰顶端,浅刺后电针连接双侧肩峰端,电针设置为虚拟;空白组大鼠不予干预。采用阴道涂片观察动情周期,每天记录体重并进行阴道学涂片,在动情前期第1日开始针刺,每日1次,针刺结束后次日结合阴道学涂片,将处于动情期大鼠处死,取腹主动脉血,检测血清雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黄体生成素(LH)、胰岛素生长因子-1(IGF-1)的表达情况。同时取大鼠双侧卵巢组织,称重,并于光学显微镜下观察所有视野中的初级卵泡、次级卵泡、成熟卵泡的数目。结果:①针刺组血清雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黄体生成素(LH)、胰岛素生长因子-1(IGF-1)值与空白组、安慰针组相比,差异有统计学意义(p0.05)。②针刺组大鼠卵巢重量及大鼠卵巢指数与空白组、安慰针组相比,差异有统计学意义(p0.05)。③针刺组初级卵泡数、次级卵泡数及卵泡总数较空白组、安慰针组多,但p0.05,差异无统计学意义;针刺组与空白组、安慰针组在初级卵泡率、次级卵泡率方面相比,差异无统计学意义(p0.05);针刺组成熟卵泡率与空白组、安慰针组相比,差异有统计学意义(p0.05)。结论:①针刺可以促进卵泡的生长、发育,提高成熟卵泡率,促进卵泡的排出。②针刺可提高血清中雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黄体生成素(LH)、胰岛素生长因子-1(IGF-1)的水平,从而改善排卵功能,提高受孕率。
[Abstract]:Objective: to explore the effect mechanism of acupuncture on follicle growth, development, maturation and excretion and its effect on reproductive endocrine hormone, to explain the scientific connotation of traditional Chinese medicine therapy, and to provide scientific basis for clinical application of acupuncture to promote ovulation. Methods: 27 rats with normal estrous cycle were randomly divided into three groups according to their body weight: blank group, acupuncture group and comfort needle group, with 9 rats in each group. The acupuncture group selected "middle pole", "air sea", "Baihui", bilateral "Sanyinjiao", bilateral "Hegu", electroacupuncture connecting "middle pole" and "air sea", and consolation needle group selected unknown acupoints, Bilateral trochanter of femur and Houhai (depression between tail root and anus), top of bilateral acromial, electroacupuncture connected to bilateral acromial end after superficial prick, electroacupuncture was set as virtual, blank group rats did not intervene. Vaginal smears were used to observe the estrus cycle, body weight was recorded and vaginal smears were taken daily. Acupuncture was performed on the first day of estrus, once a day, and the rats were killed in estrus the next day after acupuncture combined with vaginal smears. Serum estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor-1 (IGF-1) were collected from abdominal aorta. The number of primary follicles, secondary follicles and mature follicles in all visual fields were observed under optical microscope. Results Serum estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor 1 (IGF-1) were compared with those in control group and comfort needle group. The difference was statistically significant (p0.05) .2 the weight of ovary and the ovarian index of rats in acupuncture group were significantly higher than those in blank group and placebo acupuncture group (p0.05). The number of primary follicles, the number of secondary follicles and the total number of follicles in acupuncture group were significantly higher than those in blank group. There were no significant differences in primary follicle rate and secondary follicle rate between the acupuncture group and the blank group (p0.05), while the mature follicle rate in the acupuncture group was higher than that in the blank group and the placebo needle group (p0.05), while the rate of mature follicles in the acupuncture group was higher than that in the blank group and the placebo needle group (p0.05). The difference was statistically significant (p0.05). Conclusion Acupuncture can promote the growth and development of follicles, increase the rate of mature follicles, promote the excretion of follicles and increase the levels of estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor-1 (IGF-1) in serum. Thus, the ovulation function is improved and the pregnancy rate is increased.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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