电针合谷穴对面神经损伤家兔面运动神经元超微结构的影响

发布时间：2018-08-19 21:00

【摘要】：目的观察电针合谷穴对面神经损伤家兔面部行为学及面运动神经元超微结构的影响,初步探讨电针合谷穴对面神经损伤的修复作用。方法采用神经卡压法制备面神经损伤家兔模型,将16只家兔随机分为4组:假手术组、模型组、电针合谷穴组和电针后溪穴组。假手术组切开左侧面部皮肤,暴露面神经后缝合。模型组造模后不给任何处置。电针合谷穴组采用电针合谷穴治疗,电针后溪穴组予电针后溪穴治疗,每次30min,每日1次,治疗28天。肉眼观察家兔面部运动情况,透射电镜观察面运动神经元超微结构。结果假手术组:造模14、28天后,细胞器丰富,结构较完整。模型组:造模14天后,细胞水肿明显,细胞染色质边集,线粒体肿胀,粗面内质网断裂,表面核糖体脱落。变性严重的细胞核膜边界不清,线粒体、内质网和高尔基复合体溶解。造模28天后,细胞水肿较前减轻,细胞器较少,高尔基复合体轻度扩张。电针合谷穴组:造模14天后,细胞水肿较明显,细胞染色质少量边集,细胞器减少,线粒体肿胀,粗面内质网断裂,高尔基复合体轻度扩张。造模28天后,细胞轻度水肿,细胞器丰富,线粒体轻度肿胀,粗面内质网轻度扩张。电针后溪穴组:造模14天后,细胞水肿,细胞染色质凝集成块位于核膜下,细胞器减少,线粒体肿胀,粗面内质网断裂。造模28天后,细胞水肿较前减轻,出现高尔基复合体扩张等逆行性改变。结论电针合谷穴能促进面运动神经元超微结构的恢复,加快面神经损伤修复的进程。
[Abstract]:Objective to observe the effect of electroacupuncture at Hegu point on facial behavior and ultrastructure of facial motoneurons in rabbits with facial nerve injury and to explore the effect of electroacupuncture at Hegu point on repairing facial nerve injury. Methods 16 rabbits with facial nerve injury were randomly divided into 4 groups: sham-operated group, model group, electroacupuncture Hegu point group and electroacupuncture Houxi point group. In the sham operation group, the left facial skin was cut open and the facial nerve was sutured after exposure. The model group does not give any disposal after making the model. Hegu point group was treated by electroacupuncture at Hegu point, and Houxi point group was treated by electroacupuncture at Houxi acupoint for 30 mins, once a day for 28 days. The facial movement of rabbits was observed with naked eyes and the ultrastructure of facial motor neurons was observed with transmission electron microscope. Results in the sham operation group, the organelles were abundant and complete after 1428 days. Model group: after 14 days, cell edema, chromatin edge aggregation, mitochondria swelling, rough endoplasmic reticulum breakage and surface ribosome exfoliation were observed. Severe degeneration of the nuclear membrane boundary is unclear, mitochondria, endoplasmic reticulum and Golgi complex dissolved. After 28 days of modeling, cell edema was alleviated, organelles were less and Golgi complex was slightly dilated. In the group of electroacupuncture at Hegu point: after 14 days of modeling, the cell edema was more obvious, the chromatin was less, the organelle was decreased, the mitochondria was swollen, the rough endoplasmic reticulum was broken, and the Golgi complex was slightly dilated. After 28 days, the cells showed slight edema, abundant organelles, slight swelling of mitochondria and slight dilatation of rough endoplasmic reticulum. After 14 days of modeling, the cells were edema, chromatin aggregates were located under the nuclear membrane, organelles were decreased, mitochondria were swollen and rough endoplasmic reticulum was broken. After 28 days of modeling, cell edema was alleviated and retrograde changes such as Golgi complex dilatation were observed. Conclusion Electro-acupuncture at Hegu point can promote the recovery of the ultrastructure of facial motor neurons and accelerate the repair process of facial nerve injury.
【作者单位】：湖南中医药大学;湖南中医药大学第一附属医院;
【基金】：湖南省中医药管理局课题(201514)
【分类号】：R245

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