电针合谷穴对面神经损伤家兔面部运动功能及面运动神经元超微结构的影响

发布时间：2018-08-22 09:19

【摘要】：目的:探讨电针合谷穴对面神经损伤家兔面部行为学、面神经传导功能及面运动神经元超微结构的影响,初步研究合谷穴在面神经再生修复中的作用,为临床应用合谷穴恢复面神经功能提供一定的实验依据。方法:采用神经卡压法制备面神经损伤家兔模型,将40只家兔随机分为4组:假手术组、模型组、电针合谷穴组和电针后溪穴组。假手术组切开左侧面部皮肤,暴露面神经后缝合。模型组造模后不给任何处置。电针合谷穴组采用电针合谷穴治疗,电针后溪穴组予电针后溪穴治疗,每次30min,每日1次,治疗28天。肉眼观察家兔面部运动情况并评分,面神经电图检测受损面神经复合肌肉动作电位的潜伏期和峰值振幅,并采用透射电镜观察面运动神经元超微结构。结果:1.行为学评分结果:电针合谷穴组造模7、14、21、28天后行为学评分升高,与模型组及电针后溪穴组相比,差异具有统计学意义(P0.05,P0.01);2.面神经电图结果:电针合谷穴组造模14、28天后潜伏期缩短、峰值振幅增大,与模型组及电针后溪穴组相比,差异有统计学意义(P0.05,P0.01);3.面运动神经元超微结构分析:电镜下,假手术组:造模14、28天后,细胞器丰富,结构较完整。模型组:造模14天后,细胞水肿明显,细胞染色质边集,线粒体肿胀,粗面内质网断裂,表面核糖体脱落。变性严重的细胞核膜边界不清,线粒体、内质网和高尔基复合体溶解。造模28天后,细胞水肿较前减轻,细胞器较少,高尔基复合体轻度扩张。电针合谷穴组:造模14天后,细胞水肿较明显,细胞染色质少量边集,细胞器减少,线粒体肿胀,粗面内质网断裂,高尔基复合体轻度扩张。造模28天后,细胞轻度水肿,细胞器丰富,线粒体轻度肿胀,粗面内质网轻度扩张。电针后溪穴组:造模14天后,细胞水肿,细胞染色质凝集成块位于核膜下,细胞器减少,线粒体肿胀,粗面内质网断裂。造模28天后,细胞水肿较前减轻,出现高尔基复合体扩张等逆行性改变。结论:1.电针合谷穴能改善面神经损伤家兔面部行为学表现;2.电针合谷穴能促进面神经损伤家兔面部复合肌肉动作电位潜伏期、峰值振幅的恢复;3.电针合谷穴能促进面运动神经元超微结构的恢复,加快面神经损伤修复的进程。
[Abstract]:Objective: to investigate the effects of electroacupuncture at Hegu point on facial behavior, facial nerve conduction function and facial motor neuron ultrastructure in rabbits with facial nerve injury, and to study the role of Hegu point in facial nerve regeneration and repair. To provide a certain experimental basis for clinical application of Hegu point to restore facial nerve function. Methods: 40 rabbits with facial nerve injury were randomly divided into 4 groups: sham-operated group, model group, electroacupuncture Hegu point group and electroacupuncture Houxi point group. In the sham operation group, the left facial skin was cut open and the facial nerve was sutured after exposure. The model group does not give any disposal after making the model. Hegu point group was treated by electroacupuncture at Hegu point, and Houxi point group was treated by electroacupuncture at Houxi acupoint for 30 mins, once a day for 28 days. Facial motility of rabbits was observed with naked eye, the latency and peak amplitude of action potential of injured facial nerve compound muscle were detected by facial electrogram, and the ultrastructure of facial motor neurons was observed by transmission electron microscope (TEM). The result is 1: 1. Behavioral score: the behavioral score of Hegu group was higher than that of model group and Hexi point group (P0.05, P0.01) after 28 days, and the difference was statistically significant (P0.05and P0.01). The results of facial nerve electrogram showed that the latency was shortened and the peak amplitude was increased in Hegu group after 14 days. The difference was statistically significant compared with model group and Hexi point group (P0.05P0.01). Ultrastructural analysis of facial motor neurons: under electron microscope, sham-operation group: after 1428 days of modeling, the organelle was abundant and the structure was relatively complete. Model group: after 14 days, cell edema, chromatin edge aggregation, mitochondria swelling, rough endoplasmic reticulum breakage and surface ribosome exfoliation were observed. Severe degeneration of the nuclear membrane boundary is unclear, mitochondria, endoplasmic reticulum and Golgi complex dissolved. After 28 days of modeling, cell edema was alleviated, organelles were less and Golgi complex was slightly dilated. In the group of electroacupuncture at Hegu point: after 14 days of modeling, the cell edema was more obvious, the chromatin was less, the organelle was decreased, the mitochondria was swollen, the rough endoplasmic reticulum was broken, and the Golgi complex was slightly dilated. After 28 days, the cells showed slight edema, abundant organelles, slight swelling of mitochondria and slight dilatation of rough endoplasmic reticulum. After 14 days of modeling, the cells were edema, chromatin aggregates were located under the nuclear membrane, organelles were decreased, mitochondria were swollen and rough endoplasmic reticulum was broken. After 28 days of modeling, cell edema was alleviated and retrograde changes such as Golgi complex dilatation were observed. Conclusion 1. Electroacupuncture at Hegu can improve the facial behavior of rabbits with facial nerve injury. Electroacupuncture at Hegu point can promote the latency of facial compound muscle action potential and the recovery of peak amplitude of facial nerve injury in rabbits. Electroacupuncture at Hegu can promote the recovery of the ultrastructure of facial motor neurons and accelerate the repair of facial nerve injury.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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