背俞指针疗法对GERD大鼠任督二脉穴位皮温及钙调蛋白基因表达的影响

发布时间：2018-09-03 13:51

【摘要】：目的:本文拟在通过分析背俞指针疗法干预GERD大鼠任督二脉局部的皮温治疗前后变化特点和探索钙调蛋白在胃窦Cajal间质细胞内的表达情况,从中医学和医学分子生物学角度了解该病的病因病机及治疗机制,为今后该病的临床诊治提供相应的参考。方法:按照完全随机法将120只SD大鼠分为空白、模型组、对照组、治疗组进行比较,每组30只。采用贲门钢圈固定法制作GERD大鼠模型,空白组和模型组不实施干预治疗,治疗组连续14天予以实施背俞指针治疗,对照组连续14天予以兰索拉唑肠溶片联合莫沙必利分散片灌胃治疗。于治疗前及治疗结束后的的第1天、第7天及第14天应用红外热成像仪检测GERD大鼠的任督二脉穴位均温皮温变化。实验结束后,取胃中部距贲门1/3处的胃窦运动起搏区组织,采用逆转录聚合酶链反应(RT-PCR)的方法检测GERD大鼠胃窦部Cajal间质细胞中CaM基因表达情况。结果:1.任督二脉各穴位红外皮温检测结果治疗第1天,模型组、对照组、治疗组与空白组相比,大椎穴、至阳穴、命门穴、膻中穴皮温明显降低(p0.05),中脘穴、关元穴皮温明显升高(p0.05)。治疗第7天,模型组与治疗组、空白组相比,大椎穴、至阳穴、命门穴、膻中穴、中脘穴、关元穴皮温明显降低(p0.05)。与对照组相比,大椎穴、至阳穴、膻中穴、中脘穴、关元穴明显降低(p0.05),命门穴无明显降低(p0.05)。治疗第14天,模型组与对照组、治疗组相比,大椎穴、至阳穴、命门穴、膻中穴、中脘穴、关元穴皮温明显降低(p0.05)。与空白组相比,命门穴、膻中穴明显降低(p0.05)。大椎穴、至阳穴、中脘穴、关元无明显降低(p0.05)。2.任督二脉红外热均温检测结果治疗第1天,模型组、对照组、治疗组较空白组相比,任脉均温明显升高(p0.05),督脉均温明显下降(p0.05)。治疗第7天,模型组与治疗组相比,任脉均温、督脉均温明显降低(p0.05),与空白组、对照组相比,任脉均温、督脉均温无明显差异(p0.05)。治疗第14天,模型组与对照组、治疗组相比,任脉均温、督脉均温明显降低(p0.05),与空白组相比,任脉均温、督脉均温无明显差异。3.胃窦Cajal间质细胞钙调蛋白基因表达结果模型组大鼠胃窦Cajal间质细胞的CaM基因表达下调(p0.05),使用西药和背俞指针治疗后可使CaM基因表达上调(p0.05),且治疗组的CaM基因表达要显著高于对照组(p0.05)。结论:1.任督二脉穴位皮温的变化与胃食管反流病的发病相关。GERD大鼠的任督二脉穴位皮温较正常大鼠的皮温低,而使用背俞指针治疗后能够提高任督二脉的皮温。背俞指针治疗胃食管反流病有效机制可能是通过改善任督二脉经气来实现。2.GERD大鼠胃窦Cajal间质细胞钙调蛋白表达下降可能是胃食管反流病的发病机制之一。3.背俞指针治疗对GERD的治疗作用很可能是通过上调胃窦Cajal间质细胞钙调蛋白的基因表达,促进胃窦起搏区运动来实现。
[Abstract]:Objective: to explore the changes of calmodulin in the interstitial cells of Cajal in antrum of GERD rats before and after skin temperature therapy. To understand the etiology, pathogenesis and therapeutic mechanism of the disease from the point of view of Chinese medicine and medical molecular biology, and to provide the corresponding reference for the clinical diagnosis and treatment of the disease in the future. Methods: 120 SD rats were randomly divided into blank group, model group, control group and treatment group with 30 rats in each group. The GERD rat model was made by the method of cardia ring fixation. The blank group and the model group were not treated with intervention, and the treatment group was treated with backshu pointer for 14 days. The control group was treated with lansoprazole enteric-coated tablets and mosapride dispersible tablets for 14 days. The changes of mean temperature at the acupoints of Ren du and Ermai of GERD rats were detected by infrared thermography on the 1st day, 7th day and 14th day before and after treatment. At the end of the experiment, the tissue of antral motor pacing region 1 / 3 from the cardia in the middle of the stomach was taken and the expression of CaM gene in the Cajal interstitial cells of the gastric antrum of GERD rats was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The result is 1: 1. On the first day of treatment, model group, control group, treatment group, compared with the blank group, Dazhui point, Zhiyang point, Shengmen point, Shanzhong point skin temperature decreased (p0.05), Zhongwan point, Guanyuan point skin temperature increased significantly (p0.05). On the 7th day, the skin temperature at Dazhui, Zhiyang, Shengzhong, Zhongwan and Guanyuan points in the model group was significantly lower than that in the control group (p0.05). Compared with the control group, Dazhui point, Zhiyang point, Shanzhong point, Zhongwan point and Guanyuan point were significantly decreased (p0.05), but not at Shengmen point (p0.05). On the 14th day, the skin temperature at Dazhui, Zhiyang, Shengzhong, Zhongwan and Guanyuan points in the model group was significantly lower than that in the control group (p0.05). Compared with the blank group, the points of Shengzhong and Shengmen were significantly decreased (p0.05). Dazhui point, Zhiyang point, Zhongwan point, Guan Yuan have no significant decrease (p0.05) .2. On the first day of treatment, the model group, the control group, the treatment group compared with the blank group, the mean temperature of the Ren pulse increased significantly (p0.05), the mean temperature of the du pulse decreased significantly (p0.05). On the 7th day of treatment, the mean temperature of Ren pulse and du pulse in model group was significantly lower than that in treatment group (p0.05), but there was no significant difference between model group and control group (p0.05). On the 14th day, compared with the control group, the model group and the treatment group, the mean temperature of Ren pulse and du vein decreased significantly (p0.05), compared with the blank group, there was no significant difference in the mean temperature of Ren pulse and du pulse. 3. Expression of calmodulin gene in interstitial cells of antral Cajal in rats of model group, the expression of CaM gene was down-regulated (p0.05) in the stromal cells of gastric antrum Cajal. The expression of CaM gene was upregulated (p0.05) after treatment with western medicine and back-shu pointers, and the expression of CaM gene in the treatment group. It was significantly higher than that of the control group (p0.05). Conclusion 1. The change of skin temperature at acupoint of Ren du and Ermai is related to the pathogenesis of gastroesophageal reflux disease. The skin temperature of Ren du and Ermai acupoints in GERD rats is lower than that of normal rats, but the skin temperature of Ren du er vein can be increased after treatment with back Shu pointer. The effective mechanism of the treatment of gastroesophageal reflux disease with back Shu pointers may be that the decrease of calmodulin expression in the interstitial cells of gastric antrum Cajal may be one of the pathogenesis of gastroesophageal reflux disease. The therapeutic effect of back Shu pointer therapy on GERD may be achieved by upregulating the expression of calmodulin gene in stromal cells of gastric antrum Cajal and promoting the movement of gastric antral pacing area.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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