藏药臭蒿及其近缘种药材的ITS2 DNA条形码鉴别
发布时间：2018-10-08 17:30
【摘要】：臭蒿Artemisia hedinii有清热凉血,退黄,消炎的功能,在藏药中占有重要地位。蒿属植物种类繁多,在青藏高原分布广泛,且其外形较为相似,传统鉴定方法难以准确快速鉴定。文章以ITS2序列为DNA条形码,建立了一种快速准确鉴定臭蒿及其易混蒿属藏药植物的方法。从青藏高原采集蒿属藏药21个样品,分别提取样品DNA,对ITS2目标序列进行PCR扩增、测序,获得其ITS2序列信息;并从Gen Bank上下载同属序列11条;运用MEGA 6.0对所有32条ITS2序列进行对比,计算种间种内遗传距离,构建Neighbor Joining(NJ)系统进化树,并比较样本ITS2序列间的二级结构差异。结果显示:臭蒿、牛尾蒿、黄花蒿、艾蒿的遗传距离较近,但四者种间最小距离均远大于种内最大距离,NJ树显示4种蒿属植物种内之间各单独形成一个分支,ITS2序列二级结构也显示出差异。以ITS2序列作为DNA条形码,可达到对臭蒿及其近缘蒿属藏药植物进行准确、快速的识别和鉴定的目的,准确地弄清物种之间的系统进化关系的目的,为该属药用植物的质量控制、安全用药及合理开发利用提供理论依据。
[Abstract]:Artemisia odorifolia Artemisia hedinii has the function of clearing away heat and cooling blood, reducing yellow and eliminating inflammation, which plays an important role in Tibetan medicine. Artemisia is widely distributed in Qinghai-Xizang Plateau and its appearance is similar. Traditional identification methods are difficult to identify accurately and quickly. Using ITS2 sequence as DNA bar code, a rapid and accurate method for identification of Artemisia odorifolia and its easily mixed Tibetan medicinal plants was established. 21 samples of Artemisia Tibetan medicine were collected from Qinghai-Xizang Plateau. The ITS2 target sequence was amplified by PCR and sequenced by DNA, and the information of ITS2 sequence was obtained, and 11 homologous sequences were downloaded from Gen Bank. All 32 ITS2 sequences were compared by MEGA 6.0, the genetic distance between species was calculated, the phylogenetic tree of Neighbor Joining (NJ) was constructed, and the secondary structure differences among ITS2 sequences were compared. The results showed that the genetic distance of Artemisia odorifolia, Artemisia oxysporum, Artemisia annua and Artemisia argyi was close. However, the minimum distance between the four species was far greater than the maximum distance within the species, and the secondary structure of ITS2 sequence was also different among the four species of Artemisia. The ITS2 sequence can be used as DNA barcode for accurate and rapid identification and identification of Artemisia and its relative Tibetan medicinal plants, and for the quality control of medicinal plants in the genus Artemisia. Safe use of drugs and rational development and utilization to provide theoretical basis.
【作者单位】： 西南交通大学生命科学与工程学院;西南民族大学药学院;
【基金】：国家科技重大专项(2014ZX09304-307-001-019) 四川省中医药管理局项目(2016Q039)
【分类号】：R29
，
本文编号：2257655
[Abstract]:Artemisia odorifolia Artemisia hedinii has the function of clearing away heat and cooling blood, reducing yellow and eliminating inflammation, which plays an important role in Tibetan medicine. Artemisia is widely distributed in Qinghai-Xizang Plateau and its appearance is similar. Traditional identification methods are difficult to identify accurately and quickly. Using ITS2 sequence as DNA bar code, a rapid and accurate method for identification of Artemisia odorifolia and its easily mixed Tibetan medicinal plants was established. 21 samples of Artemisia Tibetan medicine were collected from Qinghai-Xizang Plateau. The ITS2 target sequence was amplified by PCR and sequenced by DNA, and the information of ITS2 sequence was obtained, and 11 homologous sequences were downloaded from Gen Bank. All 32 ITS2 sequences were compared by MEGA 6.0, the genetic distance between species was calculated, the phylogenetic tree of Neighbor Joining (NJ) was constructed, and the secondary structure differences among ITS2 sequences were compared. The results showed that the genetic distance of Artemisia odorifolia, Artemisia oxysporum, Artemisia annua and Artemisia argyi was close. However, the minimum distance between the four species was far greater than the maximum distance within the species, and the secondary structure of ITS2 sequence was also different among the four species of Artemisia. The ITS2 sequence can be used as DNA barcode for accurate and rapid identification and identification of Artemisia and its relative Tibetan medicinal plants, and for the quality control of medicinal plants in the genus Artemisia. Safe use of drugs and rational development and utilization to provide theoretical basis.
【作者单位】： 西南交通大学生命科学与工程学院;西南民族大学药学院;
【基金】：国家科技重大专项(2014ZX09304-307-001-019) 四川省中医药管理局项目(2016Q039)
【分类号】：R29
，
本文编号：2257655
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