脾胃虚弱型肥胖小鼠模型的建立与评价

发布时间：2018-10-08 19:50

【摘要】：目的:建立脾胃虚弱型肥胖小鼠模型并进行评价。方法:复制高脂饮食诱导肥胖模型,在肥胖模型的基础上按照神疲乏力、食少纳呆、便溏等主要脾虚指标进行二次筛选符合条件的肥胖鼠。通过ELISA法检测血清总胆固醇、甘油三酯、高密度脂蛋白及低密度脂蛋白含量,测定血清中炎症因子(白介素-6、白介素10及肿瘤坏死因子-α)及脾脏、胸腺和肠道派氏小体中CD4~+、CD8~+及CD11c~+等含量变化。结果:肥胖鼠成模率62.96%,其中脾胃虚弱型肥胖鼠23.53%。脾虚型肥胖鼠IL-10、TNF-α显著高于正常组,白介素-6显著低于正常组,其肠道派氏小体中的CD4~+、CD8~+及CD11c~+明显降低。结论:此方法可以较好地筛选出脾胃虚弱型肥胖鼠,为健脾益气治疗肥胖奠定基础。
[Abstract]:Objective: to establish and evaluate the model of spleen and stomach deficiency obesity mice. Methods: the obesity model was induced by high fat diet. According to the main indexes of spleen deficiency, such as fatigue of mind, low intake of food, loose stool and so on, the obese mice were screened twice according to the criteria of obesity. The levels of serum total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were measured by ELISA, and the inflammatory factors (interleukin-6, interleukin 10 and tumor necrosis factor- 伪) and spleen were measured. The changes of CD4~ CD 8 ~ and CD11c~ in thymus and intestinal body. Results: the model formation rate of obese rats was 62.96, and the spleen and stomach asthenia type obesity rats were 23.53. The levels of IL-10,TNF- 伪 and interleukin-6 in the spleen deficiency obese rats were significantly higher than those in the normal group, and the levels of CD4~ CD8 ~ and CD11c~ in the pellets were significantly lower than those in the normal group. Conclusion: this method can be used to screen the obese rats with deficiency of spleen and stomach and lay a foundation for the treatment of obesity by invigorating spleen and invigorating qi.
【作者单位】：成都中医药大学基础医学院;
【基金】：国家自然科学基金资助项目(81273899)-健脾益气祛痰法对肥胖鼠肠黏膜TLRs的良性调控机制研究
【分类号】：R-332;R259

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