心悸宁丸对大鼠室性早搏及血清NO、ET-1水平的影响

发布时间：2018-10-10 09:53

【摘要】：目的观察中药制剂心悸宁丸对肾上腺素致室性早搏大鼠模型心律、血清NO和ET-1水平的影响,探讨该药物预防室性早搏的可能作用机制。方法将48只健康雄性SD大鼠随机分为6组,每组8只,即:健康组、心悸宁丸低剂量组(0.487 g/kg)、心悸宁丸中剂量组(0.974g/kg)、心悸宁丸高剂量组(1.948g/kg)、普萘洛尔组(2mg/kg)和模型组。各组大鼠每日清晨空服灌胃给药1次,每次2ml。其中健康组和模型组每日给予同等剂量的生理盐水灌胃,连续10天。第10天末次给药1小时后,除健康组外,其余五组以鼠尾静脉快速注射盐酸肾上腺素(100μg/kg)诱发大鼠心律失常模型,记录心律失常出现时间、心律失常持续时间和大鼠心率的变化,统计各组大鼠室性早搏发生率,最后从腹腔动脉采血测定血清NO和ET-1的含量。结果与模型组相比,心悸宁丸高、中剂量组和普萘洛尔组在推迟心律失常出现时间和缩短心律失常持续时间方面作用明显,有显著性差异(P0.05);与模型组相比,心悸宁丸高、中、低各剂量组均能降低大鼠室早发生率;与模型组相比,心悸宁丸高、中、低各剂量组和普萘洛尔组均能使大鼠血清NO含量明显增加,ET-1含量明显减少,差异显著有统计学意义(P0.05)。结论心悸宁丸能较好的对抗由肾上腺素诱发的大鼠心律失常,使心律失常出现时间延迟,心律失常持续时间缩短并显著低室性早搏发生率,且与剂量大小呈正相关,中药高剂量组的拮抗作用最好;心悸宁丸能明显提高室性早搏大鼠血清NO的水平,显著降低血清ET-1的水平,保护血管内皮,对室性早搏具有良好的预防作用。
[Abstract]:Objective to observe the effects of Xinjinning pills on the cardiac rhythm, serum NO and ET-1 levels in rats with epinephrine induced ventricular premature beat (VPB), and to explore the possible mechanism of the prevention of VPB. Methods 48 healthy male SD rats were randomly divided into 6 groups: healthy group, low dose group (0.487 g/kg), moderate dose group (0.974g/kg), high dose group (1.948g/kg), propranolol group (2mg/kg) and model group. Rats in each group were given intragastric administration of 2 ml once a day in the morning. The healthy group and the model group were given the same dose of normal saline daily for 10 days. One hour after the last administration on the 10th day, the rats in the other five groups, except the healthy group, were induced by rapid injection of epinephrine hydrochloride (100 渭 g/kg) into the tail vein of rats to record the occurrence time of arrhythmia, the duration of arrhythmia and the changes of heart rate in rats. The incidence of ventricular premature beats in each group was counted and the serum NO and ET-1 levels were measured from celiac artery. Results compared with the model group, the heart palpitations were higher, the middle dose group and propranolol group had significant effects on delaying the onset of arrhythmias and shortening the duration of arrhythmia (P0.05), compared with the model group, the heart palpitation pills were high and moderate. Compared with the model group, the high, middle, low dose groups and propranolol groups could significantly increase the content of serum NO and decrease the content of ET-1, the difference was statistically significant (P0.05). Conclusion Xinjinning pills can antagonize the arrhythmias induced by adrenaline in rats, delay the occurrence of arrhythmias, shorten the duration of arrhythmias and significantly reduce the incidence of ventricular premature beats, and have a positive correlation with the dose. The antagonistic effect of high dose Chinese medicine group was the best. Xinjinning pills could significantly increase the level of serum NO, significantly reduce the level of serum ET-1, protect vascular endothelium, and have a good preventive effect on ventricular premature beats in rats.
【作者单位】：河南推拿职业学院;河南中医学院第三附属医院;河南中医学院;
【基金】：河南省中医药科学研究专项课题(重大项目)(No.2013ZY01010)
【分类号】：R285.5

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