壮医药线点灸对寒湿凝滞型痛经大鼠一氧化氮、内皮素-1的影响

发布时间：2018-10-10 19:37

【摘要】：目的:观察壮医药线点灸疗法对痛经模型大鼠的疗效及对一氧化氮(NO)、内皮素-1(ET-1)的影响,探讨其对该痛经模型治疗的可能作用机制。方法:选取12周龄雌性SD大鼠110只随机分为正常对照组(A组)、痛经模型组(B组)、壮医药线点灸组(C组)、月月舒药物组(D组)、针刺组(E组),每组22只。采用雌激素+肾上腺素注射并全身冰水浸泡法造模,观察各组大鼠注射缩宫素30min内扭体次数和扭体反应潜伏期,分别用NO还原酶法及ELISA法测定大鼠血浆、子宫匀浆中NO、ET-1水平。结果:1、与对照组比较,模型组扭体潜伏期短,扭体次数最多,有显著统计学意义(P0.01);壮医药灸组、针刺组以及药物组与模型组相比,首次扭体潜伏期均明显延长,且30 min内扭体反应次数少(P0.01);壮医药灸组与药物组、针刺组在30min内扭体次数相比较,均无统计学意义(P0.05);但壮医药灸组与药物组相比,其扭体潜伏期明显伏延长(P0.05);且三组中,壮医药灸组扭体潜伏期最长、扭体抑制率最高。2、与正常组比较,壮医药灸组、针刺组、药物组大鼠血清及子宫匀浆NO、ET-1含量无显著差异(P0.05);与模型组比较,壮医药灸组、针刺组及药物组血清及子宫匀浆ET-1显著降低,NO明显升高(P0.01);壮医药灸组、针刺组以及药物组相比,血清及子宫匀浆NO、ET值均无明显统计学差异(P0.05);结论:壮医药线点灸疗法治疗寒湿凝滞型痛经的作用机制可能与增加大鼠血清及子宫组织中NO水平、降低血清及子宫组织ET-1水平有关。
[Abstract]:Objective: to observe the effect of Zhuang medicine thread moxibustion on dysmenorrhea model rats and the effect of nitric oxide (NO), endothelin-1 (ET-1) on dysmenorrhea model. Methods: one hundred and ten female SD rats aged 12 weeks were randomly divided into 4 groups: normal control group (group A), dysmenorrhea model group (group B), moxibustion group (group C), moxibustion group (group D) and acupuncture group (group E). The rats were injected with estrogen epinephrine and soaked in ice water all over the body. The times of writhing and the latency of writhing reaction were observed by injection of oxytocin 30min. The levels of NO,ET-1 in plasma and uterine homogenate were measured by NO reductase method and ELISA method respectively. Results: 1, compared with the control group, the model group had shorter latent period of writhing and the most times of writhing (P0.01), and the first writhing latency of the Zhuang medicated moxibustion group, acupuncture group and drug group was significantly longer than that of the model group. The times of writhing reaction in 30 min were less (P0.01); the times of writhing in 30min were not significantly different between Zhuang medicinal moxibustion group and drug group and acupuncture group (P0.05); but the latent period of writhing in Zhuang medicinal moxibustion group was significantly longer than that in drug group (P0.05); in the three groups, the writhing latency of Zhuang medicinal moxibustion group was significantly longer than that of drug group (P0.05). The latent period of writhing was the longest and the inhibition rate of writhing was the highest in Zhuang medicinal moxibustion group. 2. There was no significant difference in NO,ET-1 content in serum and uterus homogenate of rats in Zhuang medicinal moxibustion group, acupuncture group and drug group (P0.05), compared with model group, Zhuang medicated moxibustion group had no significant difference (P0.05). ET-1 in serum and uterine homogenate in acupuncture group and drug group were significantly decreased and NO was significantly increased (P0.01), while that in Zhuang medicinal moxibustion group, acupuncture group and drug group was significantly higher than that in acupuncture group and drug group. There was no significant difference in NO,ET values between serum and uterine homogenate (P0.05). Conclusion: the mechanism of treating dysmenorrhea with cold dampness and stagnation may be related to the increase of NO level in serum and uterine tissue of rats. The decrease of ET-1 level in serum and uterus was related.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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