针刺不同单穴及腧穴配伍对糖尿病胃轻瘫大鼠胃窦Cajal间质细胞影响的实验研究

发布时间：2018-10-11 17:50

【摘要】：目的:探讨针刺单穴及腧穴配伍对糖尿病胃轻瘫(Diabetic gastroparesis,DGP)大鼠胃窦Cajal间质细胞(Interstitial Cells of Cajal,ICC)的影响。方法:选取雄性SD(Sprague Dawley)大鼠,以链脲佐菌素(Steptozotocin,STZ)腹腔注射诱导DGP大鼠模型,造模成功后选取大鼠60只,随机分为正常组、模型组、西药组组、足三里组、中脘+足三里组,每组10只。各治疗组治疗5周后,观察大鼠的一般情况,采用HE(Hematoylin-eosin)染色观察各组胃窦形态改变,采用免疫组化技术观察各组ICC含量的改变。结果:与正常组相比,模型组大鼠胃窦组织ICC含量明显减少(P0.01);与模型组比较,西药组(P0.01)、中脘+足三里组(P0.01)、足三里组(P0.05)、中脘组(P0.05)表达明显增多,有统计学意义;中脘+足三里组与足三里组(P0.05)、中脘组(P0.01)有明显差异,且优于其他二组;中脘组与足三里组无明显差异。结论:针刺可促进DGP大鼠胃窦ICC含量恢复,腧穴配伍组优于单穴组。
[Abstract]:Objective: to investigate the effect of acupuncture on (Interstitial Cells of Cajal,ICC of gastric antral Cajal interstitial cells in diabetic gastroparesis (Diabetic gastroparesis,DGP) rats. Methods: male SD (Sprague Dawley) rats were randomly divided into normal group, model group, western medicine group, Zusanli group and Zusanli group by intraperitoneal injection of streptozotocin (Steptozotocin,STZ), 60 rats were randomly divided into normal group, model group, western medicine group, Zusanli group and Zhongwan Zusanli group. 10 rats in each group. After 5 weeks of treatment, the general situation of rats was observed, the changes of antrum morphology were observed by HE (Hematoylin-eosin) staining, and the changes of ICC content in each group were observed by immunohistochemical technique. Results: compared with normal group, the content of ICC in gastric antrum of model group was significantly decreased (P0.01), compared with model group, the expression of ICC in western medicine group (P0.01), Zhongwan Zusanli group (P0.01), Zusanli group (P0.05), Zhongwan group (P0.05) was significantly higher than that in model group. Zhongwan Zusanli group and Zusanli group (P0.05), Zhongwan group (P0.01), and better than the other two groups, Zhongwan group and Zusanli group had no significant difference. Conclusion: acupuncture can promote the recovery of ICC content in gastric antrum of DGP rats, and the acupoint compatibility group is better than the single point group.
【学位授予单位】：长春中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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