艾灸对迷走神经切断后大鼠急性胃黏膜损伤修复的研究

发布时间：2018-10-21 12:46

【摘要】：目的:选择性地切断大鼠胃迷走神经通路,并应用组织形态学、生化及分子生物学等方法,观察艾灸预处理对胃黏膜损伤大鼠的内源性物质白细胞介素-1β(IL-1β)和表皮生长因子(EGF)、肿瘤坏死因子(TNF-α)含量的影响,以及热休克蛋白70的表达,进一步探讨艾灸预处理对胃黏膜损伤的保护作用机制和迷走神经通路之间的关系。方法:将SD大鼠随机分为四组,即A正常对照组;B模型组;C艾灸+模型组;D艾灸+模型+迷走神经切断组。手术组应严格按照要求先进行迷走神经切断,然后采用无水乙醇灌胃法对相应各组制备大鼠急性胃黏膜损伤模型,同时对各组进行艾灸处理,取胃组织后按Guth法计算胃黏膜损伤指数(UI)和光镜下观察胃黏膜组织形态学变化;最后采用酶联免疫吸附法检测胃黏膜组织保护相关内源性物质IL-1β、EGF、TNF-α的含量和蛋白质印迹法(Western-blot)检测血清中HSP70的表达。结果:与A组比较,B组UI、血清IL-1β含量明显升高(P0.01)、EGF含量下降(P0.01)、TNF-α含量明显升高(P0.01)、胃黏膜组织浸润明显、HSP70稍升高;与B组和D组比较,C组UI、血清IL-1β含量明显降低(P0.01)、TNF-α含量明显降低(P0.01)、EGF含量升高(P0.01)、胃黏膜组织形态较为完好、HSP70表达上调(P0.05)。结论:艾灸组能够明显改善大鼠胃黏膜损伤组织形态和降低胃黏膜损伤指数,同时也能上调大鼠胃黏膜当中HSP70的表达,而这一修复作用在一定程度上受到了迷走神经的调节,因而,迷走神经通路是艾灸对胃黏膜损伤修复和保护因子表达的重要调节途径。
[Abstract]:Objective: to selectively cut off the gastric vagus pathway in rats and to apply histomorphology, biochemistry and molecular biology, etc. To observe the effect of moxibustion preconditioning on the contents of interleukin 1 尾 (IL-1 尾) and epidermal growth factor (EGF), tumor necrosis factor (TNF- 伪) and the expression of heat shock protein 70 (HSP70) in rats with gastric mucosal injury. To further investigate the protective mechanism of moxibustion preconditioning on gastric mucosal injury and the relationship between vagus nerve pathway and moxibustion pretreatment. Methods: SD rats were randomly divided into four groups: a normal control group, B model group, C moxibustion model group and D moxibustion model vagotomy group. The operation group should first perform vagotomy strictly according to the requirements, and then make the model of acute gastric mucosal injury in each group by the method of anhydrous ethanol gavage, at the same time, the moxibustion treatment should be carried out in each group. The gastric mucosal injury index (UI) was calculated by Guth method and the morphologic changes of gastric mucosa were observed under light microscope. Finally, the contents of IL-1 尾 and EGF,TNF- 伪 and the expression of HSP70 in serum were detected by enzyme-linked immunosorbent assay (Elisa) and Western blot (Western-blot). Results: compared with group A, the serum IL-1 尾 content of UI, in group B was significantly increased (P0.01), EGF decreased (P0.01), the content of TNF- 伪 was significantly increased (P0.01), the infiltration of gastric mucosa was obvious, and HSP70 was slightly increased. Compared with group B and group D, serum IL-1 尾 content and TNF- 伪 content of UI, in group C decreased significantly (P0.01), and the content of TNF- 伪 increased (P0.01), EGF). The morphology of gastric mucosa was intact and the expression of HSP70 was up-regulated (P0.05). Conclusion: moxibustion group can obviously improve the morphology of gastric mucosal injury and decrease the index of gastric mucosal injury in rats. It can also up-regulate the expression of HSP70 in gastric mucosa of rats, which is regulated by vagus nerve to some extent. Therefore, vagus pathway is an important regulatory pathway of moxibustion on the repair of gastric mucosal injury and the expression of protective factors.
【作者单位】：湖南中医药大学针灸推拿学院;
【基金】：国家自然科学青年基金课题(81303050) 2014年湖南省优秀博士论文资助项目(2014-2016)
【分类号】：R245.8

【相似文献】