疏肝理肺方对慢性应激性哮喘大鼠皮质酮及MMP-9的表达影响
发布时间：2018-10-24 21:36
【摘要】：目的:通过建立慢性应激条件下的哮喘大鼠模型,从分子水平研究不同浓度疏肝理肺方对哮喘大鼠血清皮质酮(CORT)及肺组织MMP-9表达的影响,进一步探索疏肝理肺方控制慢性应激哮喘气道重塑的机制。方法:48只健康雌性SD大鼠随机分为6组:正常组(A组)、应激模型组(B组)、地塞米松组(C组)、疏肝理肺方低浓度组(D组)、疏肝理肺方中浓度组(E组)、疏肝理肺方高浓度组(F组),每组8只。除正常组外,余各组采用氢氧化铝-卵清蛋白(OVA)致敏激发哮喘,同时加用慢性不可预见性温和应激法建立慢性应激模型。造模结束后取材,HE染色观察肺组织病理学改变,ELISA法检测血清CORT的含量,Western blot法测定肺组织MMP-9的表达。结果:与A组相比,B组肺组织炎性细胞明显浸润,支气管平滑肌及基底膜增厚,粘膜肿胀,褶皱形成,与A组相比,B组支气管壁面积和气道平滑肌面积显著增高(P0.05);与B组相比较,治疗组上述症状明显减轻(P0.05);与E组相比,D、F组上述症状减轻不明显(P0.05)。Western和ELISA结果显示,C组和疏肝理肺方各浓度组上述表现较应激模型组减轻;疏肝理肺方低浓度组和高浓度组上述表现较中浓度组增强。B组CORT和MMP-9表达量较A组明显升高(P0.05);治疗组CORT和MMP-9表达量较B组降低(P0.05);D组和F组CORT和MMP-9表达量较C组和E组高(P0.05)。结论:疏肝理肺方可通过抑制血清CORT及肺组织MMP-9的表达,有效缓解慢性应激条件下的哮喘大鼠气道重塑。
[Abstract]:Objective: to study the effects of Shugan Lifei recipe (Shugan Lifei) on the expression of corticosterone (CORT) in serum and MMP-9 in lung tissue of asthmatic rats at molecular level by establishing a rat model of asthma under chronic stress. To further explore the mechanism of Shugan Lifei recipe to control airway remodeling in chronic stress asthma. Methods: 48 healthy female SD rats were randomly divided into 6 groups: normal group (group A), stress model group (group B), dexamethasone group (group C), low concentration group of Shugan Lifei prescription (group D), middle concentration group of Shugan lifei prescription (group E) and high concentration of Shugan Lifei prescription. Group F, 8 rats in each group. In addition to the normal group, the other groups were sensitized by aluminum hydroxide ovalbumin (OVA) to stimulate asthma, and the chronic stress model was established by chronic unpredictable mild stress. The pathological changes of lung tissue were observed by HE staining and the expression of MMP-9 in lung tissue was detected by, Western blot method and the content of serum CORT by ELISA method. Results: compared with group A, pulmonary inflammatory cells in group B were significantly infiltrated, bronchial smooth muscle and basement membrane thickened, mucosal swelling and fold formation. Compared with group A, the area of bronchial wall and airway smooth muscle in group B was significantly higher than that in group A (P0.05), and that in group B was higher than that in group B (P0.05). Compared with E group, the above symptoms in group D and F were not significantly alleviated (P0.05). Western and ELISA results showed that the above symptoms in group C and Shugan Lifei group were lighter than those in stress model group. The expression of CORT and MMP-9 in group B was significantly higher than that in group A (P0.05); the expression of CORT and MMP-9 in treatment group was lower than that in group B (P0.05). The expression of CORT and MMP-9 in group F and); D were higher than those in group C and E (P0.05). Conclusion: Shuganlifei decoction can effectively relieve airway remodeling of asthmatic rats under chronic stress by inhibiting the expression of serum CORT and lung tissue MMP-9.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259
[Abstract]:Objective: to study the effects of Shugan Lifei recipe (Shugan Lifei) on the expression of corticosterone (CORT) in serum and MMP-9 in lung tissue of asthmatic rats at molecular level by establishing a rat model of asthma under chronic stress. To further explore the mechanism of Shugan Lifei recipe to control airway remodeling in chronic stress asthma. Methods: 48 healthy female SD rats were randomly divided into 6 groups: normal group (group A), stress model group (group B), dexamethasone group (group C), low concentration group of Shugan Lifei prescription (group D), middle concentration group of Shugan lifei prescription (group E) and high concentration of Shugan Lifei prescription. Group F, 8 rats in each group. In addition to the normal group, the other groups were sensitized by aluminum hydroxide ovalbumin (OVA) to stimulate asthma, and the chronic stress model was established by chronic unpredictable mild stress. The pathological changes of lung tissue were observed by HE staining and the expression of MMP-9 in lung tissue was detected by, Western blot method and the content of serum CORT by ELISA method. Results: compared with group A, pulmonary inflammatory cells in group B were significantly infiltrated, bronchial smooth muscle and basement membrane thickened, mucosal swelling and fold formation. Compared with group A, the area of bronchial wall and airway smooth muscle in group B was significantly higher than that in group A (P0.05), and that in group B was higher than that in group B (P0.05). Compared with E group, the above symptoms in group D and F were not significantly alleviated (P0.05). Western and ELISA results showed that the above symptoms in group C and Shugan Lifei group were lighter than those in stress model group. The expression of CORT and MMP-9 in group B was significantly higher than that in group A (P0.05); the expression of CORT and MMP-9 in treatment group was lower than that in group B (P0.05). The expression of CORT and MMP-9 in group F and); D were higher than those in group C and E (P0.05). Conclusion: Shuganlifei decoction can effectively relieve airway remodeling of asthmatic rats under chronic stress by inhibiting the expression of serum CORT and lung tissue MMP-9.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259
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