三伏灸诱导胸腔体表不同灸温对哮喘型新西兰兔肺组织MMP-9表达的影响
发布时间：2018-11-09 08:58
【摘要】：目的：对比观察三伏灸不同灸温对哮喘型新西兰兔肺组织MMP-9蛋白及mRNA表达的影响差异,并探讨胸腔体表温度的变化与隔姜灸量的相关性,及其与气至病所的关系。方法：选择雄性新西兰兔30只,体质量(2.5±0.3)kg。采用随机数字表随机分为空白组,模型组,实验组,对照组,地塞米松组,每组6只。空白组不造模,其余各组均按照哮喘气道重塑模型制作方法进行造模。模型组仅造模,不施予治疗。实验组及对照组均给予隔姜灸联合中药敷贴干预,穴位均选择大椎及双侧肺俞,并采用红外热像仪监测两组哮喘型新西兰兔胸腔体表温度变化,两组均干预15次,隔日1次。实验组予隔姜灸至胸腔体表温度较治疗前升高1℃,对照组予隔姜灸3壮干预。地塞米松组给予0.5mg/kg地塞米松雾化吸入,干预15次,隔日1次。实验结束后,取新西兰兔右肺组织,采用免疫组织化学染色方法,检测右肺组织MMP-9蛋白表达。采用荧光定量PCR法检测左肺组织MMP-9 mRNA表达。观察5组新西兰兔MMP-9的改变情况,同时分析胸腔体表温度的变化与隔姜灸量的相关性,及其与气至病所的关系。结果：1.模型组、实验组、对照组、地塞米松组的MMP-9蛋白及mRNA表达明显高于空白组,差异均具有统计学意义(P0.05)；实验组、对照组、地塞米松组的MMP-9蛋白及mRNA表达明显低于模型组,差异均具有统计学意义(P0.05)；实验组的MMP-9蛋白及mRNA表达明显低于对照组,差异均具有统计学意义(P0.05)；实验组与地塞米松组的MMP-9蛋白及mRNA表达无明显差异,差异无统计学意义(P0.05)。2.实验组灸后的胸腔体表温度明显高于灸前的体表温度,差异具有统计学意义(P0.05)；对照组灸后的胸腔体表温度与灸前无明显差异,差异无统计学意义(P0.05)；实验组灸后的胸腔体表温度明显高于对照组灸后的胸腔体表温度,差异具有统计学意义(P0.05)；实验组的胸腔体表温度随着隔姜灸壮数的增加而升高,spearman相关系数r=0.433,具有显著性差异(P0.01)。结论：隔姜灸达到9壮及以上可能通过升高哮喘型新西兰兔胸腔体表温度而诱导出气至病所,从而下调肺组织MMP=9蛋白及mRNA表达,抑制MMP-9的合成,改善哮喘型新西兰兔气道重塑,从而有效地缓解哮喘症状。同时,胸腔体表温度与隔姜灸的壮数呈正相关,表明温度的提升可能为隔姜灸时达到气至病所的相关指标之一。
[Abstract]:Objective: to observe the effect of different moxibustion temperature on the expression of MMP-9 protein and mRNA in lung tissue of asthmatic New Zealand rabbits, and to explore the relationship between the surface temperature of thoracic cavity and the quantity of ginger separated moxibustion. Methods: 30 male New Zealand rabbits with body weight of (2.5 卤0.3) kg. were selected. Six rats were randomly divided into blank group, model group, experimental group, control group and dexamethasone group. No model was made in the blank group, and the other groups were made according to the airway remodeling model of asthma. In the model group, only the model was made and not treated. The experimental group and the control group were treated with ginger separated moxibustion combined with traditional Chinese medicine application intervention. Dazhui and bilateral Feshu were selected at acupoints, and the changes of chest surface temperature of asthmatic New Zealand rabbits were monitored by infrared thermograph. The intervention was 15 times in both groups, once every other day. The experimental group was treated with ginger-separated moxibustion to chest surface temperature 1 鈩，
本文编号：2319952
[Abstract]:Objective: to observe the effect of different moxibustion temperature on the expression of MMP-9 protein and mRNA in lung tissue of asthmatic New Zealand rabbits, and to explore the relationship between the surface temperature of thoracic cavity and the quantity of ginger separated moxibustion. Methods: 30 male New Zealand rabbits with body weight of (2.5 卤0.3) kg. were selected. Six rats were randomly divided into blank group, model group, experimental group, control group and dexamethasone group. No model was made in the blank group, and the other groups were made according to the airway remodeling model of asthma. In the model group, only the model was made and not treated. The experimental group and the control group were treated with ginger separated moxibustion combined with traditional Chinese medicine application intervention. Dazhui and bilateral Feshu were selected at acupoints, and the changes of chest surface temperature of asthmatic New Zealand rabbits were monitored by infrared thermograph. The intervention was 15 times in both groups, once every other day. The experimental group was treated with ginger-separated moxibustion to chest surface temperature 1 鈩，
本文编号：2319952
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