蒙药嘎日迪散对溃疡性结肠炎大鼠Th1细胞和Th17细胞的影响

发布时间：2018-11-09 17:18

【摘要】：目的探讨蒙药嘎日迪散对溃疡性结肠炎(UC)大鼠Th1细胞和Th17细胞的影响。方法将Wistar大鼠随机分为正常组、UC模型组、嘎日迪散低剂量组、嘎日迪散中剂量组、嘎日迪散高剂量组、柳氮磺吡啶组和补脾益肠丸组,采用免疫法加2,4,6-三硝基苯磺酸乙醇诱导UC模型。嘎日迪散低、中、高剂量组(1.76、3.51、7.02 g/kg),柳氮磺吡啶组(0.36 g/kg)和补脾益肠丸组(1.62g/kg)分别灌胃相应药物4周后,取外周血检测指标。采用流式细胞术观察全血中Th1细胞/Th17细胞(Th1/Th17),ELISA法检测血清中转化生长因子β(TGF-β)、白细胞介素6(IL-6)、白细胞介素17(IL-17)、干扰素γ(IFN-γ)的含量。结果嘎日迪散中、低剂量和柳氮磺吡啶能显著降低UC大鼠血清中TGF-β,各药物均能显著降低UC大鼠血清中IL-6、IL-17的含量(P0.05)、升高IFN-γ的含量(P0.05),升高Th1/Th17值(P0.05)。结论嘎日迪散调节UC大鼠体内Th0细胞的分化方向,恢复Th1/Th17的平衡可能是其治疗UC的机制之一。
[Abstract]:Objective to investigate the effect of Garidi Powder on Th1 cells and Th17 cells in (UC) rats with ulcerative colitis. Methods Wistar rats were randomly divided into normal group, UC model group, low dose group, medium dose group, high dose group, sulfasalopyridine group and Bupiyichang pill group. UC model was induced by 6-trinitrobenzene sulfonate ethanol. Gari Di San was low, medium and high dose group (1.76 ~ 3.51 ~ 7.02 g/kg), Salazapyridine group (0.36 g/kg) and Bupiyichang pill group (1.62g/kg) were given by stomach for 4 weeks, and the peripheral blood was measured. Serum levels of transforming growth factor 尾 (TGF- 尾), interleukin-6 (IL-6) and interleukin-17 (IL-17) were measured by flow cytometry in Th1 / Th17 cells (Th1/Th17), ELISA method). The content of interferon 纬 (IFN- 纬). Results in the middle and middle dose of Gari Di San, low dose and sulfasalopyridine significantly decreased TGF- 尾 in serum of UC rats, and all drugs significantly decreased the content of IL-6,IL-17 in serum of UC rats (P0.05), and increased the content of IFN- 纬 (P0.05). The Th1/Th17 value was increased (P0.05). Conclusion Garidi powder may regulate the differentiation of Th0 cells and restore the balance of Th1/Th17 in UC rats, which may be one of the mechanisms of its treatment of UC.
【作者单位】：中央民族大学中国少数民族传统医学研究院;新乡医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金项目(No.81273872)~~
【分类号】：R29

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