针刺对支气管哮喘大鼠肺组织CollagenⅠ、Fibronection、VEGF表达的影响

发布时间：2018-11-11 08:58

【摘要】：目的通过观察针刺对支气管哮喘大鼠肺组织Col I、FN、VEGF表达的影响,以期明确Col I、FN、VEGF与哮喘的关系以及针刺对其的调控作用。方法将90只大鼠按照随机数字表法分成3组,每组30只,分别为空白组、模型组、针刺组。采用腹腔注射OVA和Al(OH)3混合液致敏后雾化吸入OVA溶液激发法制备哮喘模型。空白组雾化吸入生理盐水,针刺组于针刺后雾化吸入OVA溶液。根据总干预时间的差异,分别于雾化2、4周后,按照随机数字表法从每组大鼠中选出10只处死,于雾化6周后处死剩余大鼠。空白组雾化2、4、6周大鼠分别记为A、B、C,模型组雾化2、4、6周大鼠分别记为D、E、F,针刺组针刺2、4、6周大鼠分别记为G、H、I。通过HE染色、Masson染色观察各组大鼠肺组织的病理改变情况,通过免疫组化法、ELISA法观察各组大鼠肺组织Col I、FN、VEGF的表达情况。结果1模型组大鼠在接受激动剂雾化激发时,出现烦躁不安、呼吸加快加深、口唇紫绀、二便失禁等症状,针刺组和空白组大鼠在雾化时上述症状较轻。2各组大鼠肺组织HE染色显示,模型组大鼠肺组织出现严重的气道重塑及气道炎症病理改变,且随着雾化周期的增加D、E、F组肺组织病理改变程度增加。针刺组及空白组大鼠肺组织无明显病理改变,且随着雾化周期的增加肺组织结构无明显变化。3各组大鼠肺组织Masson染色显示,模型组大鼠肺组织WA/Pi、WAm/Pi、WAc/Pi、WAi/Pi平均值均较空白组增大(P0.05),随着雾化周期的增长,D、E、F组WA/Pi、WAm/Pi、WAi/Pi的均值呈增长趋势,WAc/Pi无随雾化周期增长而增厚的现象。针刺组与空白组大鼠肺组织WA/Pi、WAm/Pi、WAc/Pi、WAi/Pi平均值无显著差异(P0.05)。4各组大鼠肺组织免疫组化法、ELISA法检测显示,模型组大鼠肺组织Col I、FN、VEGF的阳性表达明显高于空白组(P0.05),且随着雾化周期的延长D、E、F各组Col I、FN、VEGF的表达呈下降趋势,但仍较空白组高(P0.05),差异有统计学意义。针刺组大鼠肺组织Col I、FN、VEGF的表达低于模型组(P0.05),差异有统计学意义。结论1针刺可以显著改善卵蛋白雾化激发引起的哮喘大鼠呼吸困难、口唇青紫、烦躁不安等症状。2随着雾化激发周期的增加,模型组D、E、F小组大鼠肺组织气道重塑程度逐渐加重,而针刺可以明显抑制哮喘大鼠气道重塑的发生发展。3哮喘模型大鼠肺组织Col I、FN、VEGF的表达增多,且随着雾化激发次数的增多哮喘大鼠肺组织Col I、FN、VEGF的表达水平呈下降趋势。4针刺可以抑制哮喘大鼠肺组织Col I、FN、VEGF的表达。
[Abstract]:Objective to observe the effect of acupuncture on the expression of Col in lung tissue of asthmatic rats, in order to clarify the relationship between Col and asthma and the regulatory effect of acupuncture on it. Methods 90 rats were randomly divided into 3 groups: blank group, model group and acupuncture group. Asthma models were induced by intraperitoneal injection of OVA and Al (OH) _ 3 and then inhaled by OVA solution. The blank group inhaled normal saline, and the acupuncture group inhaled OVA solution after acupuncture. According to the difference of total intervention time, 10 rats were selected from each group according to random number table method after 4 weeks of atomization, and the remaining rats were killed after 6 weeks of atomization. The rats in the blank group were recorded as Acarinic at 6 weeks, the rats in the model group in the atomized group at 2 and 4 weeks were recorded as DGV EfU, and the rats in the acupuncture group were recorded as GG HG I at the 6th week of acupuncture. The pathological changes of lung tissue were observed by HE staining and Masson staining, and the expression of Col IfNNtr in lung tissue of each group was observed by immunohistochemical method and ELISA method. Results 1 the rats in the model group had symptoms such as agitation, rapid breathing, cyanosis and incontinence when they were stimulated by agonist atomization, and the rats in the model group had symptoms such as restlessness, rapid breathing, cyanosis of the lips, and incontinence. The above symptoms were mild in acupuncture group and blank group. (2) HE staining in lung tissue of rats in each group showed severe airway remodeling and pathological changes of airway inflammation in model group, and with the increase of atomization cycle, the lung tissue of model group showed severe pathological changes of airway remodeling and airway inflammation. The pathological changes of lung tissue in group F were increased. There were no obvious pathological changes in lung tissue in acupuncture group and blank group, and there was no obvious change in lung tissue structure with the increase of atomization cycle. 3 Masson staining of lung tissue in each group showed that WA/Pi,WAm/Pi,WAc/Pi, in lung tissue of model group was not changed. Compared with the blank group, the average value of WAi/Pi increased (P0.05). With the increase of atomization cycle, the average value of WA/Pi,WAm/Pi,WAi/Pi in DIAE E F group showed an increasing trend, and WAc/Pi did not increase with the increase of atomization cycle. There was no significant difference in WA/Pi,WAm/Pi,WAc/Pi,WAi/Pi between the acupuncture group and the blank group (P0.05). 4 the lung tissue of each group was detected by immunohistochemical method and ELISA method. The positive expression of VEGF was significantly higher than that of the blank group (P0.05), and the expression of VEGF in Col Igna FNN was decreased with the prolongation of the atomization cycle, but still higher than that in the blank group (P0.05), the difference was statistically significant. The expression of Col in lung tissue in acupuncture group was lower than that in model group (P0.05). Conclusion (1) Acupuncture can significantly improve the symptoms of asthmatic rats induced by ovalbumin atomization, such as dyspnea, purplish lip, agitation, etc. The degree of airway remodeling in group F was gradually aggravated, while acupuncture could significantly inhibit the development of airway remodeling in asthmatic rats. 3 the expression of Col in lung tissue of asthmatic rats was increased. The expression of Col in lung tissue of asthmatic rats was decreased with the increase of the times of atomization stimulation. 4 Acupuncture could inhibit the expression of Col in lung tissue of asthmatic rats.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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