基于数据挖掘的脑梗塞恢复期组方规律研究与涤痰通瘀方对急性出血性脑卒中肝损害IRAK-4的干预
发布时间：2018-11-14 17:11
【摘要】：研究目的目的一通过对中医药治疗脑梗塞恢复期处方的用药进行数据挖掘,分析中药治疗脑梗塞恢复期的组方规律,为临床合理有效地遣方用药提供一定的数据支持和参考依据。对其遣方用药规律进行提炼浓缩,同时观察西医学范畴内脑梗塞恢复期与中医中风病之间的关联。目的二探讨涤痰通瘀方在急性出血性脑卒中大鼠肝损害的作用白细胞介素-1受体相关激酶-4(IRAK-4)活性的表达。研究方法方法一收集关于中国知网、万方数据库脑梗塞恢复期的临床相关文献,并借助V2.5平台建立数据库,应用软件中"数据分析系统"模块的配方,分析数据库建设中涉及的处方频率、处方规则分析和新处方分析。方法二采用健康雄性成年SD大鼠,通过酶消化法、密度梯度离心以及选择贴壁法提取KC,随机分为正常组,脑出血组,涤痰通瘀方组,每组分6h,12h,24h,48h 4个时相亚组,通过实时荧光定量PCR观察肝组织IRAK-4 mRNA表达,酶联免疫吸附试验(ELISA)检测培养上清液中TNF-α的含量。研究结果(1)临床文献研究最终筛选出符合标准的文献137篇,得到处方220个。220个处方涉及中药225味,出现频次位于前十位的为川芎、当归、地龙、水蛭、黄芪、丹参、红花、石菖蒲、桃仁、赤芍,常用的药物组合有"川芎,当归"、"川芎,地龙"、"川芎,黄芪"等,核心药物是川芎、当归、地龙和水蛭,进而演化出核心组合16个,得到新方12个。(2)实验研究涤痰通瘀方抑制急性出血性脑卒中大鼠肝KCs细胞IRAK-4 mRNA表达,并能明显减轻TNF-α的含量,中药组IRAK-4蛋白表达水平与上清液TNF-α水平呈正相关。研究结论(1)临床文献研究结论中国知网和万方数据库上所收录的脑梗塞恢复期的处方中使用药物以益气活血通络为主。常用方剂为解语丹、补阳还五汤、通窍活血汤等。(2)实验研究结论IRAK-4是LPS的细胞内信号转导的重要调节因子;涤痰通瘀方抑制IRAK-4激酶的活性,随之引起下游关键炎症因子TNF-α的产生明显减少,因此,涤痰通瘀方抑制KCs的IRAK-4的激活是减轻肝损害的重要机制之一。
[Abstract]:Objective: to analyze the rule of prescription of traditional Chinese medicine in the recovery period of cerebral infarction through the data mining of the prescription of traditional Chinese medicine in the recovery period of cerebral infarction. To provide certain data support and reference basis for clinical rational and effective prescription. At the same time, the relationship between cerebral infarction convalescence and stroke in traditional Chinese medicine was observed. Objective to investigate the effect of Ditan Tongyu recipe on liver injury in rats with acute hemorrhagic stroke and to investigate the expression of interleukin-1 receptor associated kinase-4 (IRAK-4) activity. Methods one was to collect the clinical literature about the recovery period of cerebral infarction in Chinese Zhiwang and Wanfang database, and establish the database with the help of V2.5 platform, and apply the formula of "data analysis system" module in the software. This paper analyzes the prescription frequency, prescription rules and new prescriptions involved in database construction. Methods the healthy male adult SD rats were randomly divided into normal group, intracerebral hemorrhage group, Ditan Tongyu prescription group, each group with 4 subgroups: normal group, intracerebral hemorrhage group, Ditan Tongyu prescription group, each group with enzyme digestion, density gradient centrifugation and selective adherent method, and each group was divided into 4 subgroups at 6 h, 12 h, 24 h and 48 h, respectively. The expression of IRAK-4 mRNA in liver tissue was observed by real-time fluorescence quantitative PCR and the content of TNF- 伪 in culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). Results (1) in clinical literature research, 137 articles were selected and 220 prescriptions were obtained. 225 kinds of traditional Chinese medicines were obtained. The first ten prescriptions were Rhizoma Chuanxiong, Angelica sinensis, Leech, Radix Astragali, Salvia miltiorrhiza. The common drug combinations are "Chuanxiong, Angelica sinensis", "Chuanxiong, Dilong", "Chuanxiong, Huangqi" and so on. The core drugs are Chuanxiong, Angelica, Dilong and Hirudo, and then evolved 16 core combinations. Twelve new prescriptions were obtained. (2) the experimental study showed that Ditan Tongyu decoction inhibited the expression of IRAK-4 mRNA in KCs cells of liver of rats with acute hemorrhagic stroke, and significantly reduced the content of TNF- 伪. There was a positive correlation between the expression of IRAK-4 protein and the level of TNF- 伪 in supernatant. Conclusion (1) Clinical literature conclusion the prescription of recovery period of cerebral infarction included in Chinese Zhiwang and Wanfang database is mainly used to invigorate qi and activate blood circulation to clear collaterals. The commonly used prescriptions are Jieyudan, Buyang Huanwu decoction, Tongqiao Huoxue decoction and so on. (2) Experimental study suggests that IRAK-4 is an important regulatory factor of intracellular signal transduction of LPS; Ditan Tongyu recipe inhibits the activity of IRAK-4 kinase, which causes the production of TNF- 伪, a key inflammatory factor in the downstream, which is obviously reduced. Therefore, Ditan Tongyu prescription inhibits the activation of IRAK-4 of KCs is one of the important mechanisms to alleviate liver damage.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R277.7
本文编号：2331790
[Abstract]:Objective: to analyze the rule of prescription of traditional Chinese medicine in the recovery period of cerebral infarction through the data mining of the prescription of traditional Chinese medicine in the recovery period of cerebral infarction. To provide certain data support and reference basis for clinical rational and effective prescription. At the same time, the relationship between cerebral infarction convalescence and stroke in traditional Chinese medicine was observed. Objective to investigate the effect of Ditan Tongyu recipe on liver injury in rats with acute hemorrhagic stroke and to investigate the expression of interleukin-1 receptor associated kinase-4 (IRAK-4) activity. Methods one was to collect the clinical literature about the recovery period of cerebral infarction in Chinese Zhiwang and Wanfang database, and establish the database with the help of V2.5 platform, and apply the formula of "data analysis system" module in the software. This paper analyzes the prescription frequency, prescription rules and new prescriptions involved in database construction. Methods the healthy male adult SD rats were randomly divided into normal group, intracerebral hemorrhage group, Ditan Tongyu prescription group, each group with 4 subgroups: normal group, intracerebral hemorrhage group, Ditan Tongyu prescription group, each group with enzyme digestion, density gradient centrifugation and selective adherent method, and each group was divided into 4 subgroups at 6 h, 12 h, 24 h and 48 h, respectively. The expression of IRAK-4 mRNA in liver tissue was observed by real-time fluorescence quantitative PCR and the content of TNF- 伪 in culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). Results (1) in clinical literature research, 137 articles were selected and 220 prescriptions were obtained. 225 kinds of traditional Chinese medicines were obtained. The first ten prescriptions were Rhizoma Chuanxiong, Angelica sinensis, Leech, Radix Astragali, Salvia miltiorrhiza. The common drug combinations are "Chuanxiong, Angelica sinensis", "Chuanxiong, Dilong", "Chuanxiong, Huangqi" and so on. The core drugs are Chuanxiong, Angelica, Dilong and Hirudo, and then evolved 16 core combinations. Twelve new prescriptions were obtained. (2) the experimental study showed that Ditan Tongyu decoction inhibited the expression of IRAK-4 mRNA in KCs cells of liver of rats with acute hemorrhagic stroke, and significantly reduced the content of TNF- 伪. There was a positive correlation between the expression of IRAK-4 protein and the level of TNF- 伪 in supernatant. Conclusion (1) Clinical literature conclusion the prescription of recovery period of cerebral infarction included in Chinese Zhiwang and Wanfang database is mainly used to invigorate qi and activate blood circulation to clear collaterals. The commonly used prescriptions are Jieyudan, Buyang Huanwu decoction, Tongqiao Huoxue decoction and so on. (2) Experimental study suggests that IRAK-4 is an important regulatory factor of intracellular signal transduction of LPS; Ditan Tongyu recipe inhibits the activity of IRAK-4 kinase, which causes the production of TNF- 伪, a key inflammatory factor in the downstream, which is obviously reduced. Therefore, Ditan Tongyu prescription inhibits the activation of IRAK-4 of KCs is one of the important mechanisms to alleviate liver damage.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R277.7
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