小艾炷直接灸“肝俞”穴对HCC大鼠成模期血清肝功能及AFP、TNF-α的影响
发布时间：2018-11-16 09:43
【摘要】：原发性肝癌(HepatocellularCarcinoma,HCC)是全球高发病率和高死亡率的恶性肿瘤之一。相关文献研究表明,灸法防治肝癌有一定的临床疗效基础,并且灸法防治疾病已有几千年的历史。因此,本研究采用小艾炷逆灸大鼠的"肝俞"穴,观察直接灸和隔姜灸对大鼠肝功能及肿瘤相关因子的影响,探讨逆灸法对原发性肝癌的预防作用。目的本实验以DEN诱导的HCC模型成模期大鼠为研究对象,以"肝俞"穴为施灸部位,造模的同时对大鼠进行直接灸和隔姜灸干预。观察直接灸和隔姜灸法对HCC模型成模期大鼠肝脏病理组织形态学,以及肝功能天冬氨酸氨基转移酶(Aspartate transaminase,AST),γ-谷氨酰转肽酶(Glutamyl transpeptidase,GGT)、碱性磷酸酶(Alkaline phosphatas,ALP)、甲胎蛋白(Alpha-fetoproteinheterop-lasmon,AFP)和肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)的影响,以探讨该灸法对HCC模型大鼠成模期的预防作用,并评价两种灸量以及不同灸法对HCC模型大鼠的预防效应。方法将70只清洁级wistar雄性大鼠随机分为空白组(10只)、模型组(15只)、直接灸1组(15只)、直接灸2组(15只)、隔姜灸组(15只)。除空白组外,各组大鼠按照50mglkg的剂量,腹腔注射浓度为l0mg/ml的DEN溶液,每周2次,每次间隔3或4d(约84h),连续10周;直接灸1组灸"肝俞"穴,3壮/穴;直接灸2组灸"肝俞"穴,6壮/穴,均隔日1次,连续10周,每壮艾炷灸完再换;隔姜灸组需在"肝俞"穴上放置一 2mm厚的姜片,灸"肝俞"穴,3壮/穴,每壮艾炷灸完再换;造模结束后,对大鼠肝脏病理形态学变化用HE染色法观察;用全自动生化分析仪对各组大鼠血清AST、GGT、ALP进行检测,用ELISA法对各组大鼠血清中AFP、TNF-α进行检测。结果1肝组织病理形态学变化:空白组大鼠肝细胞胞浆胞核均匀、肝细胞索排列整齐,呈放射状,肝细胞形态正常;模型组肿瘤细胞成团块状分布,小叶结构完全破坏,核/质比增大,细胞核深染,异型性增加,肝细胞坏死,部分出现核固缩和核碎裂现象;直接灸1组,核/质比略增大,肝癌细胞数量较少,细胞核异型性低,有少量结节形成;直接灸2组,细胞核深染、核/质比增大,肝细胞有双核;隔姜灸组,细胞核深染、核/质比略增大,有肝细胞索和肝血窦结构。2血清肝功能表达情况:与空白组相比,模型组大鼠血清AST、GGT、ALP含量均显著升高(P0.05);与模型组相比,直接灸1组、直接灸2组大鼠血清GGT含量均显著降低(P0.05),隔姜灸组大鼠血清AST、GGT、ALP含量均显著降低(P0.05)。3血清AFP表达情况:与空白组相比,模型组大鼠血清AFP含量显著增高(P0.05);与模型组比较,直接灸1组、隔姜灸组大鼠的血清AFP含量均显著降低(P0.05)。4血清TNF-α表达情况:与空白组比较,模型组大鼠血清TNF-α含量显著增高(P0.05);与模型组比较,直接灸1组大鼠血清TNF-α含量显著降低(P0.05)。结论1小艾炷直接灸和隔姜灸均能改善HCC大鼠成模期的肝脏病理形态,减缓细胞癌变进程。2小艾炷直接灸和隔姜灸均能有效降低HCC大鼠成模期血清GGT含量。3直接灸1组能有效降低HCC大鼠成模期血清AFP和TNF-α含量,说明直接灸1组可以通过减缓DEN对大鼠肝细胞的炎性反应,抑制肝癌细胞的增长。4小艾炷隔姜灸能有效降低HCC大鼠成模期血清AST、GGT、ALP及AFP含量,说明隔姜灸组能够明显改善大鼠的肝功能,减轻肝细胞的损伤,抑制肝癌细胞的增长。5小艾炷直接灸和隔姜灸在一定程度上对HCC模型大鼠具有预防作用,可以减缓肝脏癌变进程,其内在机制可能是通过减轻DEN对大鼠肝细胞的炎性反应、改善肝细胞膜通透性、调节TNF-α介导的信号通路等几方面去降低肝细胞的癌变程度。
[Abstract]:Primary liver cancer (HCC) is one of the most high-and high-mortality malignant tumors in the world. The relevant literature studies have shown that the moxibustion method has a certain clinical curative effect on the prevention and treatment of liver cancer, and the moxibustion method has a history of several thousand years. Therefore, the effect of direct moxibustion and ginger moxibustion on the liver function and tumor-related factors in rats was observed by using the "liver Yu" point of the small-Ai-type counter-moxibustion rats, and the prevention effect of the reverse-moxibustion method on the primary liver cancer was discussed. Objective To study the effect of direct moxibustion and moxibustion on the rats by using DEN-induced HCC model as the study object and using the "liver Yu" point as the moxibustion part and making the model. The morphology of the liver and the morphology of liver and the liver function aspartate aminotransferase (GGT), alkaline phosphatase (ALP) and alpha-fetoroteinhaetop-lamon were observed in the model of HCC. The effect of the moxibustion on the model period of HCC model rats was discussed, and the preventive effects of different moxibustion methods on the model rats of HCC were evaluated. Methods 70 healthy male rats were randomly divided into blank group (n = 10), model group (15), direct-moxibustion group 1 (15), direct-moxibustion group 2 (15), and ginger-type moxibustion group (15 only). In addition to the blank group, the rats in each group were given a dose of 50mglkg, a DEN solution with a concentration of l0mg/ ml in the abdominal cavity, 2 times a week, 3 or 4 days at a time (about 84h), for 10 weeks for a continuous period of time, 1 group of moxibustion "liver Yu" of the direct-moxibustion group and 3 strong/ a hole, and the direct-moxibustion 2-group moxibustion "liver Yu" point, the 6-strong/ acupoint and the soaking time are once, In order to change the pathological changes of the liver of the rats, the pathological changes of the liver of the rats were observed with HE staining. The serum AST, GGT and ALP of each group were detected by a full-automatic biochemical analyzer, and AFP and TNF-1 in the serum of each group were detected by ELISA. Results: The changes of the pathological morphology of the liver tissue were as follows: the cytoplasm of the hepatocytes in the blank group was homogeneous, the liver cells were arranged in order, and the liver cells were normal; the tumor cells of the model group were clustered and distributed, the leaflet structure was completely destroyed, the nuclear/ mass ratio was increased, and the cell nucleus was deeply stained. in that direct-moxibustion group 1, the nuclear-to-mass ratio is slightly increase, the number of the liver cancer cells is small, the nuclear atypia is low, and a small number of nodules are formed; the direct-moxibustion group 2, the nuclear deep dye and the nuclear/ mass ratio increase, The serum AST, GGT and ALP in the model group were significantly higher than that of the blank group (P0.05). The levels of serum GGT in the rats of the direct-moxibustion group and the direct-moxibustion group were significantly lower (P0.05). The serum levels of AST, GGT and ALP in the rats were significantly lower than that of the blank group (P0.05). The serum AFP level in the model group was significantly higher than that of the blank group (P0.05); and compared with the model group, The levels of serum AFP in the rats were significantly lower than those in the blank group (P0.05). The serum TNF-1 content in the model group was significantly higher in the model group than in the blank group (P0.05); and compared with the model group, The serum TNF-1 content in the 1 group of the direct-moxibustion group was significantly lower (P0.05). Conclusion: 1. The direct moxibustion and the moxibustion with ginger can improve the pathological morphology of the liver in the model of HCC. in ord to slow down that process of cell canceration, the serum GGT content in the rat model of HCC can be effectively reduced by the direct moxibustion and the direct moxibustion, and the direct moxibustion 1 group can effectively reduce the content of serum AFP and TNF-1 in the model phase of the rat with HCC, and the direct moxibustion group 1 can reduce the inflammatory reaction of DEN to the rat liver cells. The increase of serum AST, GGT, ALP and AFP in the rat model of HCC can be effectively reduced, and the effect of the ginger moxibustion group on the liver function of the rat can be obviously improved, and the damage of the liver cells can be reduced. It is possible to reduce the inflammatory response of DEN to the rat's liver cells and to improve the permeability of the liver cell membrane. TNF-mediated signaling pathway and the like are regulated to reduce the degree of carcinogenesis of the liver cells.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245
本文编号：2335168
[Abstract]:Primary liver cancer (HCC) is one of the most high-and high-mortality malignant tumors in the world. The relevant literature studies have shown that the moxibustion method has a certain clinical curative effect on the prevention and treatment of liver cancer, and the moxibustion method has a history of several thousand years. Therefore, the effect of direct moxibustion and ginger moxibustion on the liver function and tumor-related factors in rats was observed by using the "liver Yu" point of the small-Ai-type counter-moxibustion rats, and the prevention effect of the reverse-moxibustion method on the primary liver cancer was discussed. Objective To study the effect of direct moxibustion and moxibustion on the rats by using DEN-induced HCC model as the study object and using the "liver Yu" point as the moxibustion part and making the model. The morphology of the liver and the morphology of liver and the liver function aspartate aminotransferase (GGT), alkaline phosphatase (ALP) and alpha-fetoroteinhaetop-lamon were observed in the model of HCC. The effect of the moxibustion on the model period of HCC model rats was discussed, and the preventive effects of different moxibustion methods on the model rats of HCC were evaluated. Methods 70 healthy male rats were randomly divided into blank group (n = 10), model group (15), direct-moxibustion group 1 (15), direct-moxibustion group 2 (15), and ginger-type moxibustion group (15 only). In addition to the blank group, the rats in each group were given a dose of 50mglkg, a DEN solution with a concentration of l0mg/ ml in the abdominal cavity, 2 times a week, 3 or 4 days at a time (about 84h), for 10 weeks for a continuous period of time, 1 group of moxibustion "liver Yu" of the direct-moxibustion group and 3 strong/ a hole, and the direct-moxibustion 2-group moxibustion "liver Yu" point, the 6-strong/ acupoint and the soaking time are once, In order to change the pathological changes of the liver of the rats, the pathological changes of the liver of the rats were observed with HE staining. The serum AST, GGT and ALP of each group were detected by a full-automatic biochemical analyzer, and AFP and TNF-1 in the serum of each group were detected by ELISA. Results: The changes of the pathological morphology of the liver tissue were as follows: the cytoplasm of the hepatocytes in the blank group was homogeneous, the liver cells were arranged in order, and the liver cells were normal; the tumor cells of the model group were clustered and distributed, the leaflet structure was completely destroyed, the nuclear/ mass ratio was increased, and the cell nucleus was deeply stained. in that direct-moxibustion group 1, the nuclear-to-mass ratio is slightly increase, the number of the liver cancer cells is small, the nuclear atypia is low, and a small number of nodules are formed; the direct-moxibustion group 2, the nuclear deep dye and the nuclear/ mass ratio increase, The serum AST, GGT and ALP in the model group were significantly higher than that of the blank group (P0.05). The levels of serum GGT in the rats of the direct-moxibustion group and the direct-moxibustion group were significantly lower (P0.05). The serum levels of AST, GGT and ALP in the rats were significantly lower than that of the blank group (P0.05). The serum AFP level in the model group was significantly higher than that of the blank group (P0.05); and compared with the model group, The levels of serum AFP in the rats were significantly lower than those in the blank group (P0.05). The serum TNF-1 content in the model group was significantly higher in the model group than in the blank group (P0.05); and compared with the model group, The serum TNF-1 content in the 1 group of the direct-moxibustion group was significantly lower (P0.05). Conclusion: 1. The direct moxibustion and the moxibustion with ginger can improve the pathological morphology of the liver in the model of HCC. in ord to slow down that process of cell canceration, the serum GGT content in the rat model of HCC can be effectively reduced by the direct moxibustion and the direct moxibustion, and the direct moxibustion 1 group can effectively reduce the content of serum AFP and TNF-1 in the model phase of the rat with HCC, and the direct moxibustion group 1 can reduce the inflammatory reaction of DEN to the rat liver cells. The increase of serum AST, GGT, ALP and AFP in the rat model of HCC can be effectively reduced, and the effect of the ginger moxibustion group on the liver function of the rat can be obviously improved, and the damage of the liver cells can be reduced. It is possible to reduce the inflammatory response of DEN to the rat's liver cells and to improve the permeability of the liver cell membrane. TNF-mediated signaling pathway and the like are regulated to reduce the degree of carcinogenesis of the liver cells.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245
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