白芥子涂法穴位贴敷对豚鼠支气管哮喘气道高反应性和肺组织TRPV1表达影响的研究

发布时间：2018-11-19 18:34

【摘要】：目的:研究白芥子涂法穴位贴敷对哮喘豚鼠的抗气道高反应性作用,并初步探讨其机制。方法:将豚鼠分为正常组、哮喘组、贴敷治疗组。模型采用OVA致敏法,首次激发后,行每周一次的白芥子涂法贴敷,共3次。末次激发后24 h,小动物呼吸功能测试仪检测呼气阻力、吸气阻力、肺顺应性,HE染色检测肺组织炎症,免疫组化法检测肺组织TRPV1的定位与表达,免疫印迹法检测肺组织TRPV1蛋白的表达水平。结果:与正常组相比,哮喘组豚鼠呼气阻力、吸气阻力明显上升,肺顺应性明显下降,TRPV1表达略有上升(P0.05);与哮喘组相比,贴敷治疗组豚鼠哮喘组豚鼠呼气阻力、吸气阻力显著下降,肺顺应性上升,TRPV1表达显著下调(P0.05)。结论:白芥子涂法穴位贴敷可能通过调节肺组织TRPV1,降低哮喘中的气道高反应性感性。
[Abstract]:Aim: to study the anti-airway hyperresponsiveness of white mustard-coated acupoint on asthmatic guinea pigs and to explore its mechanism. Methods: the guinea pigs were divided into normal group, asthma group and application treatment group. The model was sensitized by OVA. After the first excitation, the white mustard coating was applied once a week for 3 times. Breath resistance, inspiratory resistance, lung compliance, HE staining and lung inflammation were detected 24 hours after the last challenge. The localization and expression of TRPV1 in lung tissue were detected by immunohistochemical method. The expression of TRPV1 protein was detected by Western blot. Results: compared with the normal group, the expiratory resistance, inspiratory resistance and lung compliance of asthmatic guinea pigs increased significantly, and the expression of TRPV1 increased slightly (P0.05). Compared with the asthma group, the airway resistance, inspiratory resistance, lung compliance and TRPV1 expression were significantly decreased in the treatment group (P0.05). Conclusion: acupoint application with white mustard coating may reduce airway hyperresponsiveness by regulating TRPV1, in lung tissue.
【作者单位】：辽宁中医药大学;
【基金】：国家自然科学基金青年项目(81603528) 沈阳市科技局重点实验室项目(F13-285-1-00)
【分类号】：R245

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