藏药水柏枝的质量标准研究

发布时间：2018-11-25 21:48

【摘要】：目的:建立藏药水柏枝的质量标准。方法:从性状特征、显微特征、薄层色谱(TLC)方面对药材进行定性鉴别;检测药材水分、灰分和浸出物的含量;采用高效液相色谱法(HPLC)测定药材中没食子酸的含量:色谱柱为Diamonsil C_(18),流动相为乙腈-0.1%磷酸溶液(5∶95,V/V),流速为1.0 mL/min,检测波长为270 nm,柱温为30℃,进样量为10μL。结果:水柏枝呈圆柱形,有多数窄条形互生小叶,质脆,易折断,气微,味淡。木栓细胞表面和下表皮细胞表面可见多角形,木纤维多成束,草酸钙簇晶众多。TLC图斑点清晰,分离度良好。水分为6.83%~8.12%,总灰分为4.01%~5.01%,酸不溶性灰分为1.06%~1.98%,水浸出物为17.91%~22.65%,醇浸出物为11.29%~15.51%。没食子酸检测质量浓度线性范围为3.13~50μg/mL(r=0.999 7),精密度、稳定性、重复试验的RSD1.0%,没食子酸加样回收率为94.0%~100.8%(RSD=2.27%,n=9)。结论:该研究所建标准可用于藏药水柏枝的质量评价。
[Abstract]:Objective: to establish the quality standard of the Tibetan medicine Prunus chinensis. Methods: qualitative identification of medicinal materials was carried out from the aspects of characters, microscopic characteristics, TLC (TLC), and the contents of water, ash and extracts were detected. The content of Gallic acid in medicinal materials was determined by (HPLC). The chromatographic column was Diamonsil C _ (18), the mobile phase was acetonitrile-0.1% phosphoric acid (5: 95 V / V), the flow rate was 1.0 mL/min,. The detection wavelength is 270 nm, the column temperature is 30 鈩，

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