针刺对DMBA诱导的乳腺癌大鼠ER、PR表达的影响

发布时间：2018-11-27 08:17

【摘要】：目的:观察针刺疗法对二甲基苯蒽(DMBA)诱导的乳腺癌大鼠乳腺组织中雌激素受体(ER)、孕激素受体(PR)表达的影响,探索针刺防治乳腺癌的作用机制。方法:将120只6~8周龄的SD雌性大鼠随机分为模型组90只和空白组30只。模型组采用DMBA进行灌胃造模,空白组给予等剂量的芝麻油灌胃。造模15周后,将模型组大鼠随机分为治疗组和对照组。治疗组采用针刺足三里、三阴交、膻中治疗,每周3次,每次20min,共治疗12周。对照组和空白组不采用任何治疗,仅在相同时间给予抓放。针刺治疗结束后(第27周),采用电化学发光免疫方法,取腹腔静脉血做血清肿瘤标志物检测;计数肿瘤包块数量并记录形态,取包块组织,用精度为1mm的游标卡尺测量包块的高度、最大直径和相垂直的直径;采用Olympus光学显微镜下观察肿瘤组织病理学变化及ER、PR的阳性面积及平均光密度。结果:治疗组治疗后乳腺肿瘤平均数目和平均体积与对照组和空白组比较,差异均具有统计学意义(P0.01,P0.05);对照组治疗后乳腺肿瘤平均数目和平均体积与空白组比较,差异均具有统计学意义(P0.01);治疗组和对照组治疗后各项肿瘤标志物(CA724、CA125、CA199、AEP、CA15-3、CEA及CA50)浓度与空白组比较,差异均具有统计学意义(P0.01,P0.05);治疗组治疗后CA15-3浓度与对照组比较,差异具有统计学意义(P0.01);治疗组治疗后ER、PR阳性面积和平均光密度与对照组和空白组比较,差异均具有统计学意义(P0.01);对照组治疗后ER、PR阳性面积和平均光密度与空白组比较,差异均具有统计学意义(P0.01)。结论:针刺能够减少DMBA诱导的大鼠乳腺肿瘤的发生(包括肿瘤数目及肿瘤体积的大小),其作用机制可能与降低CA724、CA125、CA199、CA15-3、AEP、CEA、CA50等肿瘤标志物的浓度,尤其是降低CA15-3的浓度,减少ER、PR的阳性面积和平均光密度相关。
[Abstract]:Aim: to observe the effect of acupuncture on the expression of estrogen receptor (ER),) progesterone receptor (PR) in breast tissues of rats with breast cancer induced by dimethyl benzene anthracene (DMBA), and to explore the mechanism of acupuncture in preventing and treating breast cancer. Methods: 120 SD female rats aged 6 to 8 weeks were randomly divided into model group (n = 90) and blank group (n = 30). In the model group, DMBA was used to make the model, and the blank group was given the same dose of sesame oil. After 15 weeks, the rats in the model group were randomly divided into treatment group and control group. The treatment group was treated with acupuncture at Zusanli, Sanyinjiao and Tanzhong three times a week for 20 mins for 12 weeks. Control group and blank group did not receive any treatment, only at the same time. At the end of acupuncture (27 weeks), the serum tumor markers were detected by electrochemiluminescence immunoassay (ECI). The number of tumor masses was counted and the shape was recorded, and the tissue of the tumor was taken. The height, maximum diameter and vertical diameter of the mass were measured with Vernier caliper with precision of 1mm. The histopathological changes, positive area and average optical density of ER,PR were observed under Olympus optical microscope. Results: the mean number and volume of breast tumors in the treatment group were significantly different from those in the control group and the blank group (P 0.05). The mean number and volume of breast tumors in the control group were significantly different from those in the blank group (P0.01). The concentrations of tumor markers (CA724,CA125,CA199,AEP,CA15-3,CEA and CA50) in the treatment group and the control group were significantly different from those in the blank group (P 0.05). The concentration of CA15-3 in the treatment group was significantly higher than that in the control group (P0.01). The positive area and average optical density of ER,PR in the treatment group were significantly higher than those in the control group and the blank group (P0.01). The positive area and average optical density of ER,PR in the control group were significantly different from those in the control group (P0.01). Conclusion: acupuncture can reduce the occurrence of breast tumor (including tumor number and tumor volume) induced by DMBA in rats. The mechanism of acupuncture may be related to decreasing the concentration of tumor markers such as CA724,CA125,CA199,CA15-3,AEP,CEA,CA50. Especially, decreasing the concentration of CA15-3 and decreasing the positive area of ER,PR were related to the average optical density.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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