不同蜂针剂量对类风湿性关节炎大鼠成纤维样滑膜细胞活力的影响
发布时间：2018-12-08 12:21
【摘要】：目的:观察不同剂量的蜂针对佐剂诱导的类风湿性关节炎大鼠成纤维滑膜细胞活力的影响,探讨蜂针治疗类风湿性关节炎的可能机制。方法:建立大鼠佐剂性类风湿性关节炎动物模型,将Wistar大鼠随机分为空白组、模型组和单蜂1 min组、双蜂1 min组、三蜂1 min组、单蜂30 s组,观察各组足底厚度以及HE染色的病理变化,同时关节滑膜取材,采用MTT法检测成纤维样滑膜细胞增殖情况。结果:足底厚度比较,1 min蜂针组较模型组以及其他蜂针剂量组别低(P0.01);HE病理形态学评分,模型组病理形态学改变评分较各蜂针治疗组高(P0.05);MTT检测成纤维滑膜细胞活力中,24 h时间点开始出现,1 min蜂针组抑制成纤维细胞增殖较模型组显著(P0.05),48 h较24 h时间点各组差异更显著。结论:适当剂量的蜂针能减轻类风湿性关节炎大鼠足底厚度,降低病理形态学评分,其机制可能跟蜂针抑制成纤维样滑膜细胞增殖活力有关。
[Abstract]:Aim: to observe the effects of different doses of bee on the viability of fibroblast synoviocytes induced by adjuvant in rats with rheumatoid arthritis (RA), and to explore the possible mechanism of bee acupuncture in treating rheumatoid arthritis (RA). Methods: animal models of adjuvant rheumatoid arthritis were established in rats. Wistar rats were randomly divided into blank group, model group and single bee 1 min group, double bee 1 min group, three bees 1 min group and single bee 30 s group. The thickness of plantar and the pathological changes of HE staining were observed. Meanwhile, the synovial membrane of the joint was taken and the proliferation of fibroid synovial cells was detected by MTT method. Results: compared with the plantar thickness, 1 min bee needle group was lower than the model group and other bee needle dose groups (P0.01); HE pathomorphology score, the model group was higher than each bee needle treatment group (P0.05); The proliferation of fibroblasts was inhibited by MTT at 24 h, and the inhibition of fibroblast proliferation in min group was significantly higher than that in model group (P0.05), and the difference at 48 h was more significant than that at 24 h. Conclusion: the appropriate dose of bee acupuncture can reduce the plantar thickness and the pathological morphology score of rheumatoid arthritis rats. The mechanism may be related to the inhibition of fibroid synovial cell proliferation by bee needle.
【作者单位】： 南方医科大学中医药学院;南方医科大学中西医结合医院;
【基金】：国家自然科学基金(81303047) 广东省科学技术厅—广东省中医药科学院联合科研项目(2014A020221117) 广东省中医药局科研项目(20171170)
【分类号】：R245
[Abstract]:Aim: to observe the effects of different doses of bee on the viability of fibroblast synoviocytes induced by adjuvant in rats with rheumatoid arthritis (RA), and to explore the possible mechanism of bee acupuncture in treating rheumatoid arthritis (RA). Methods: animal models of adjuvant rheumatoid arthritis were established in rats. Wistar rats were randomly divided into blank group, model group and single bee 1 min group, double bee 1 min group, three bees 1 min group and single bee 30 s group. The thickness of plantar and the pathological changes of HE staining were observed. Meanwhile, the synovial membrane of the joint was taken and the proliferation of fibroid synovial cells was detected by MTT method. Results: compared with the plantar thickness, 1 min bee needle group was lower than the model group and other bee needle dose groups (P0.01); HE pathomorphology score, the model group was higher than each bee needle treatment group (P0.05); The proliferation of fibroblasts was inhibited by MTT at 24 h, and the inhibition of fibroblast proliferation in min group was significantly higher than that in model group (P0.05), and the difference at 48 h was more significant than that at 24 h. Conclusion: the appropriate dose of bee acupuncture can reduce the plantar thickness and the pathological morphology score of rheumatoid arthritis rats. The mechanism may be related to the inhibition of fibroid synovial cell proliferation by bee needle.
【作者单位】： 南方医科大学中医药学院;南方医科大学中西医结合医院;
【基金】：国家自然科学基金(81303047) 广东省科学技术厅—广东省中医药科学院联合科研项目(2014A020221117) 广东省中医药局科研项目(20171170)
【分类号】：R245
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