煎煮方式对满药木鸡汤药对溶出组分的影响

发布时间：2018-12-14 07:18

【摘要】：目的构建核桃楸皮的HPLC指纹图谱并研究不同溶剂与煎煮方式对满药木鸡汤(核桃楸皮-云芝)药对活性组分溶出的影响。方法利用核桃楸皮的3种有效组分胡桃醌、槲皮素、山奈酚的标准品构建HPLC指纹图谱,分别以乙酸乙酯、甲醇、水为提取溶剂进行活性组分提取,研究单煎与合煎2种提取方式所得提取物的组分差异。结果乙酸乙酯、甲醇、水3种提取溶剂的核桃楸皮单煎提取率分别为8.17%、18.51%、14.77%,均高于药对的合煎提取率3.89%、10.90%、14.24%,合煎提取会影响核桃楸皮活性组分的溶出。基于HPLC指纹图谱确定了核桃楸皮中胡桃醌、槲皮素、山奈酚3种功能组分的单煎得率与合煎得率比值,在甲醇提取中比值为2.3、2.2、2.9,在乙酸乙酯提取中分别为3.2、2.5、2.5,在水提取中分别为4.9、2.3、1.4,与药对中的云芝合煎提取会影响核桃楸皮功能组分的溶出。基于HPLC指纹图谱发现,在3种溶剂提取结果中,药对合煎提取均会造成核桃楸皮中的部分功能组分不能有效溶出,同时也在合煎提取物中检测到新组分。结论木鸡汤(核桃楸皮-云芝)药对的合煎提取会降低核桃楸皮功能组分的浸出,分煎提取后将活性组分合并使用可提高核桃楸皮功能组分的使用效率。
[Abstract]:Objective to construct the HPLC fingerprint of Juglans mandshurica bark and to study the effects of different solvents and decoction methods on the dissolution of active components in Manchurian Muji decoction (Juglans mandshurica L.). Methods the HPLC fingerprint was constructed by using three effective components of Juglans mandshurica bark, namely, walnut quinone, quercetin and kaempferol. The active components were extracted by ethyl acetate, methanol and water, respectively. To study the difference of the components of the two extraction methods: single decoction and combined decoction. Results the extraction rate of single decoction of Juglans mandshurica with ethyl acetate, methanol and water was 8.17, 18.51 and 14.77, respectively, which was higher than the combined decoction rate of 3.89 and 14.24. The extraction of mixed decoction will affect the dissolution of the active components of Juglans mandshurica bark. Based on HPLC fingerprint, the ratio of single and combined fractionation rate of three functional components of Juglans mandshurica bark, quercetin and kaempferol, was determined, and the ratio in methanol extraction was 2.3%, 2.2% and 2.9%, respectively. In the extraction of ethyl acetate, it was 3.2kW 2.5C 2.5, and in the water extraction it was 4.9m 2.31.4. The extraction of the functional components of Juglans mandshurica bark by combined decoction with the medicine pair could influence the dissolution of the functional components. On the basis of HPLC fingerprint, it was found that in the three solvent extraction results, some functional components of Juglans mandshurica bark could not be effectively dissolved, and a new component was also detected in the extract of Juglans mandshurica. Conclusion the combined decoction can reduce the extraction of functional components of Juglans mandshurica bark. The combination of the active components can improve the efficiency of the functional components of Juglans mandshurica bark.
【作者单位】：大连民族大学生命科学学院生物技术与资源利用-教育部重点实验室;
【基金】：国家自然科学基金项目(21372037;21272031;21172028) 中央高校基本科研业务费专项资金资助项目(DC201501020101;DC201502020201) 大学生科研基金(tyn2016107)
【分类号】：R29

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