朝医三神散对血管性痴呆大鼠学习记忆及细胞凋亡的实验研究
发布时间：2018-12-17 19:30
【摘要】：目的：1.探究朝医三神散对血管性痴呆大鼠(VaD)学习以及记忆能力和空间探索能力的影响；2.探究朝医三神散对血管性痴呆大鼠大脑海马体的细胞形状、排列的影响；3.探究朝医三神散及对血管性痴呆大鼠大脑海马体CAI区神经元细胞超微结构、细胞凋亡的影响。方法：取SD大鼠40只,雌雄各半,按雌雄随机分为假手术组、模型组、喜得镇对照组(西药组)、朝医三神散中剂量组、朝医三神散高剂量组。采用双侧颈总动脉动脉夹闭制备血管性痴呆(VaD)大鼠模型,除了假手术组外,均予注射硝普钠及进行双侧颈动脉夹闭,手术结束后第二天开始每天定时给予上述五组灌胃,连续28天,药品名字及剂量如下：假手术组予蒸馏水10ml/kg、模型组予蒸馏水10ml/kg、喜得镇对照组(西药组)予喜得镇溶液0.6mg/kg、朝医三神散中剂量组予朝医三神散9g/kg、朝医三神散高剂量组给予朝医三神散18g/kg灌胃,灌胃最后五天应用morris水迷宫检测血管性痴呆大鼠治疗前后行为学改变；后予灌流取脑制作石蜡切片,应用HE染色观察海马CA1区神经元的结构形态改变并对神经元数目进行定量分析。应用原位末端标记法(TUNEL)检测细胞凋亡。结果：1.经过Morris水迷宫实验研究表明,假手术组与模型组相比在学习记忆能力及空间探索能力均有统计学意义(P0.05)。模型组与喜得镇组、朝医三神散高剂量组均有统计学意义(P0.05)。朝医三神散中剂量组与模型组在水迷宫学习记忆能力实验中第一天和第四天有统计学意义(P0.05),喜得镇组与朝医三神散高剂量组、朝医三神散中剂量组之间无统计学意义(P0.05)2.经过HE染色可见,假手术组海马CA1区细胞大部分为椭圆形,排列整齐,大小一致,模型组海马CA1区细胞排列紊乱,形态各异,喜得镇组、朝医三神散高剂量组、朝医三神散中剂量组与模型组相比较,细胞排列较模型组整齐,椭圆形大小细胞较模型组多见。3.经过TUNEL染色及海马CA1区细胞凋亡计数,假手术组与模型组相比,具有统计学意义(P0.05),模型组与喜得镇组、朝医三神散高剂量组、朝医三神散中剂量组相比均有统计学意义(P0.05),喜得镇组、朝医三神散高剂量组、朝医三神散中剂量组之间无统计学意义(P0.05)。结论：1.朝医三神散对血管性痴呆大鼠学习记忆有改善作用,2.朝医三神散能够改善血管性痴呆大鼠细胞的结构和形态,3.朝医三神散对血管性痴呆大鼠海马体CAI区神经元细胞的凋亡有部分恢复作用。
[Abstract]:Objective: 1. To explore the effect of Chaoyi San Shen San on (VaD) learning, memory ability and spatial exploration ability in vascular dementia rats. 2. To explore the effect of Chaoyi San Shen San on the shape and arrangement of hippocampal cells in rats with vascular dementia. To explore the effect of Chaoyi San Shen San on the ultrastructure and apoptosis of neurons in CAI region of cerebral hippocampus of vascular dementia rats. Methods: forty SD rats, male and female, were randomly divided into sham-operation group, model group, Xidazen control group (western medicine group), middle dose group of Chaoyi Sanshen powder and high dose group of Chaoyi Sanshen powder. The (VaD) rat model of vascular dementia was established by bilateral common carotid artery occlusion. Except for sham operation group, sodium nitroprusside was injected and bilateral carotid artery occlusion was performed. For 28 consecutive days, the drug name and dosage were as follows: sham operation group was given 10 ml / kg distilled water, model group was treated with distilled water 10 ml / kg, and the control group (western medicine group) was given 0.6 mg / kg Xide solution. The middle dose group was given 9 g / kg Chaoyi Sanshen Powder, and the high dose group was given 18g/kg. The behavioral changes of vascular dementia rats before and after treatment were detected by morris water maze on the last five days. The brain was then perfused to make paraffin sections. The structural and morphological changes of neurons in the hippocampal CA1 region were observed by HE staining and the number of neurons was quantitatively analyzed. Apoptosis was detected by in situ end labeling (TUNEL). Results: 1. Morris water maze experiment showed that the sham-operation group compared with the model group in learning and memory ability and spatial exploration ability were statistically significant (P0.05). Model group and Xidazhen group, Chaoyi Sanshen high dose group were statistically significant (P0.05). The middle dose group and model group had statistical significance in the first day and the fourth day of the experiment of learning and memory ability of water maze (P0.05), the high dose group of Xide Zhen group and the high dose group of Chaoyi Sanshen Powder (P0.05), and the model group had statistical significance in the experiment of learning and memory ability of water maze (P0.05). There was no significant difference between the two groups (P0.05). HE staining showed that most of the CA1 cells in the hippocampus of sham-operation group were oval, neatly arranged and uniform in size. In the model group, the cells in the CA1 area of hippocampus were disordered and varied in shape, in the Xidazhen group, and in the high dose group of Chaoyi Sanshen Powder. Compared with the model group, the cells were arranged neatly in the middle dose group of Chaoyi Sanshen Powder, and the oval cells were more than those in the model group. After TUNEL staining and apoptosis count in hippocampal CA1 area, there was significant difference between sham operation group and model group (P0.05). There was no significant difference between the middle dose group (P0.05), the high dose group (P 0.05), the high dose group (P 0.05) and the middle dose group (P 0.05). Conclusion: 1. Chaoyi San Shen San can improve the learning and memory of vascular dementia rats, 2. Chaoyi San Shen San can improve the structure and morphology of vascular dementia rats. Chaoyi San Shen San had partial recovery effect on apoptosis of CAI neurons in hippocampus of vascular dementia rats.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29
本文编号：2384698
[Abstract]:Objective: 1. To explore the effect of Chaoyi San Shen San on (VaD) learning, memory ability and spatial exploration ability in vascular dementia rats. 2. To explore the effect of Chaoyi San Shen San on the shape and arrangement of hippocampal cells in rats with vascular dementia. To explore the effect of Chaoyi San Shen San on the ultrastructure and apoptosis of neurons in CAI region of cerebral hippocampus of vascular dementia rats. Methods: forty SD rats, male and female, were randomly divided into sham-operation group, model group, Xidazen control group (western medicine group), middle dose group of Chaoyi Sanshen powder and high dose group of Chaoyi Sanshen powder. The (VaD) rat model of vascular dementia was established by bilateral common carotid artery occlusion. Except for sham operation group, sodium nitroprusside was injected and bilateral carotid artery occlusion was performed. For 28 consecutive days, the drug name and dosage were as follows: sham operation group was given 10 ml / kg distilled water, model group was treated with distilled water 10 ml / kg, and the control group (western medicine group) was given 0.6 mg / kg Xide solution. The middle dose group was given 9 g / kg Chaoyi Sanshen Powder, and the high dose group was given 18g/kg. The behavioral changes of vascular dementia rats before and after treatment were detected by morris water maze on the last five days. The brain was then perfused to make paraffin sections. The structural and morphological changes of neurons in the hippocampal CA1 region were observed by HE staining and the number of neurons was quantitatively analyzed. Apoptosis was detected by in situ end labeling (TUNEL). Results: 1. Morris water maze experiment showed that the sham-operation group compared with the model group in learning and memory ability and spatial exploration ability were statistically significant (P0.05). Model group and Xidazhen group, Chaoyi Sanshen high dose group were statistically significant (P0.05). The middle dose group and model group had statistical significance in the first day and the fourth day of the experiment of learning and memory ability of water maze (P0.05), the high dose group of Xide Zhen group and the high dose group of Chaoyi Sanshen Powder (P0.05), and the model group had statistical significance in the experiment of learning and memory ability of water maze (P0.05). There was no significant difference between the two groups (P0.05). HE staining showed that most of the CA1 cells in the hippocampus of sham-operation group were oval, neatly arranged and uniform in size. In the model group, the cells in the CA1 area of hippocampus were disordered and varied in shape, in the Xidazhen group, and in the high dose group of Chaoyi Sanshen Powder. Compared with the model group, the cells were arranged neatly in the middle dose group of Chaoyi Sanshen Powder, and the oval cells were more than those in the model group. After TUNEL staining and apoptosis count in hippocampal CA1 area, there was significant difference between sham operation group and model group (P0.05). There was no significant difference between the middle dose group (P0.05), the high dose group (P 0.05), the high dose group (P 0.05) and the middle dose group (P 0.05). Conclusion: 1. Chaoyi San Shen San can improve the learning and memory of vascular dementia rats, 2. Chaoyi San Shen San can improve the structure and morphology of vascular dementia rats. Chaoyi San Shen San had partial recovery effect on apoptosis of CAI neurons in hippocampus of vascular dementia rats.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29
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