穴位注射脂肪来源基质血管成分结合针刺足三里抗皮肤老化的实验研究
发布时间：2018-12-28 16:13
【摘要】：目的探讨穴位注射脂肪来源基质血管成分(SVF)联合针刺足三里穴位抗皮肤老化作用及其作用机制。方法采用I型胶原酶溶液及红细胞裂解液制备SVF细胞悬液,染色后采用倒置显微镜测定SVF存活率,采用血球计数板细胞计数法进行SVF计数。24只18月龄SD大鼠随机分为四组A组、B组、C组和D组。A组大鼠足三里穴位单次注射PBS, B组大鼠针刺足三里穴位10日,C组大鼠足三里穴位单次注射SVF细胞悬液,D组大鼠足三里穴位注射SVF细胞悬液后针刺10日。各组大鼠治疗10日脱椎处死后取得各组皮肤组织。各组大鼠脱臼处死后分别取背部部分皮肤组织,匀浆后分别采用黄嘌呤氧化酶法、二甲氨基苯甲醛法、硫代巴比妥酸法和ELISA法测定皮肤组织SOD活性、羟脯氨酸、丙二醛含量和透明质酸水平。各组大鼠取其余皮肤组织常规石蜡切片,HE染色后比较各组大鼠皮肤厚度；采用兔抗人八因子相关抗原(Ⅷ-R Ag)多克隆抗体(1：100)行免疫组化染色后比较各组大鼠皮肤血管化程度。结果(1)B组和C组大鼠皮肤组织SOD活性、羟脯氨酸和透明质酸含量均较A组大鼠显著升高(P＜0.05),B组和C组大鼠皮肤组织丙二醛含量较A组大鼠显著降低(P＜0.05)；D组大鼠皮肤SOD活性、羟脯氨酸和透明质酸含量并且较B组和C组大鼠显著增加(P0.05),D组大鼠皮肤丙二醛含量较B组和C组大鼠显著降低(P＜0.05)；(2)B组和C组大鼠皮肤厚度和血管化程度较A组大鼠显著增加(P0.05)；D组大鼠皮肤厚度和微血管数目较B组和C组大鼠显著增加(P＜0.05)。结论穴位注射脂肪来源基质血管成分(SVF)联合针刺足三里通过增加SOD活性、羟脯氨酸含量,以及降低丙二醛,从而提高皮肤组织的抗氧化防御能力；通过SVF促进皮肤血管化；通过增加透明质酸和胶原蛋白含量,从而提高皮肤厚度；两者结合具有更好的抗皮肤老化作用。
[Abstract]:Objective to investigate the anti-aging effect of (SVF) combined with acupuncture at Zusanli acupoint and its mechanism. Methods SVF cell suspension was prepared by using type I collagenase solution and erythrocyte lysis solution. The survival rate of SVF was determined by inverted microscope after staining. 24 18-month-old SD rats were randomly divided into four groups: group A, group B, group C and group D. Group A rats were injected at Zusanli acupoint of group A with single injection of PBS, B for 10 days, and the rats in group B were treated with acupuncture at Zusanli acupoint for 10 days. Group C rats were treated with single injection of SVF cell suspension at Zusanli point and group D rats were treated with SVF cell suspension at point Zusanli for 10 days. The skin tissues of each group were obtained after 10 days of treatment. After the rats were killed dislocated, the skin tissues of each group were removed, and the skin tissue SOD activity and hydroxyproline were measured by xanthine oxidase method, dimethylaminobenzaldehyde method, thiobarbituric acid method and ELISA method respectively. Malondialdehyde content and hyaluronic acid level. The normal paraffin sections of other skin tissues were taken from the rats in each group, and the skin thickness of each group was compared after HE staining. The degree of skin vascularization in each group was compared by immunohistochemical staining with rabbit anti-human factor eight related antigen (鈪，
本文编号：2394161
[Abstract]:Objective to investigate the anti-aging effect of (SVF) combined with acupuncture at Zusanli acupoint and its mechanism. Methods SVF cell suspension was prepared by using type I collagenase solution and erythrocyte lysis solution. The survival rate of SVF was determined by inverted microscope after staining. 24 18-month-old SD rats were randomly divided into four groups: group A, group B, group C and group D. Group A rats were injected at Zusanli acupoint of group A with single injection of PBS, B for 10 days, and the rats in group B were treated with acupuncture at Zusanli acupoint for 10 days. Group C rats were treated with single injection of SVF cell suspension at Zusanli point and group D rats were treated with SVF cell suspension at point Zusanli for 10 days. The skin tissues of each group were obtained after 10 days of treatment. After the rats were killed dislocated, the skin tissues of each group were removed, and the skin tissue SOD activity and hydroxyproline were measured by xanthine oxidase method, dimethylaminobenzaldehyde method, thiobarbituric acid method and ELISA method respectively. Malondialdehyde content and hyaluronic acid level. The normal paraffin sections of other skin tissues were taken from the rats in each group, and the skin thickness of each group was compared after HE staining. The degree of skin vascularization in each group was compared by immunohistochemical staining with rabbit anti-human factor eight related antigen (鈪，
本文编号：2394161
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