电针“委中”对布比卡因致大鼠腰多裂肌损伤后形态学及CK、IL-17表达的影响

发布时间：2019-01-06 07:06

【摘要】：目的:观察电针"委中"穴对布比卡因(bupivacaine,BPVC)致大鼠腰多裂肌损伤后组织形态学及磷酸肌酸激酶(CK)、白介素17(IL-17)表达水平的影响。方法:将32只雄性SD大鼠随机分为对照组、模型组、电针委中组和电针肾俞组,每组8只。模型组、电针委中组和电针肾俞组采用0.5%BPVC肌内注射制备多裂肌损伤模型;对照组采用同样的方法注射0.9%Na Cl溶液。电针委中组、电针肾俞穴分别电针"委中"与"肾俞"穴,选用疏密波,频率2 Hz/10 Hz,电流强度1~2 m A,持续20 min;对照组与模型组不进行针刺干预。电针干预14 d后,通过苏木精-伊红(HE)染色和马松三色染色法(Masson)观察多裂肌炎细胞计数、瘢痕面积和肌纤维横截面积的变化。通过酶联免疫吸附法(ELISA)检测血清CK活性和IL-17的含量,并用免疫组化法检测多裂肌损伤部位的IL-17表达。结果:干预后,模型组、电针委中组和电针肾俞组的炎细胞计数、瘢痕面积明显高于对照组(均P0.01),肌纤维横截面积明显减少(均P0.01);电针委中组和电针肾俞组炎细胞计数、瘢痕面积均少于模型组(均P0.01),肌纤维横截面积大于模型组(P0.01,P0.05)。干预后,模型组、电针委中组和电针肾俞组多裂肌损伤局部的IL-17表达、血清IL-17含量及CK活性均明显高于对照组(均P0.01);电针委中组和电针肾俞组多裂肌中IL-17的表达、血清IL-17含量及CK活性均低于模型组(P0.01,P0.05);与电针肾俞组比较,电针委中组下调IL-17的趋势更明显(P0.01)。结论:电针"委中"穴可通过下调血清CK和白介素-17的过度表达,减轻炎性反应,促进多裂肌的良性修复。
[Abstract]:Aim: to observe the effect of electroacupuncture on histomorphology and expression of (CK), interleukin 17 (IL-17) of phosphocreatine kinase (CK),) after injury of lumbar polyfissure muscle induced by bupivacaine (bupivacaine,BPVC) in rats. Methods: 32 male SD rats were randomly divided into control group, model group, electroacupuncture group and Shenshu group with 8 rats in each group. Model group, electroacupuncture group and Shenshu group were injected intramuscularly with 0.5%BPVC to make the injury model of polyfissure muscle, while the control group were injected with 0.9%Na Cl solution in the same way. Electroacupuncture group and Shenshu point were treated with "Wei Zhong" and "Shen Shu", respectively, with the frequency of 2 Hz/10 Hz, and the frequency of 2 Hz/10 Hz,. The control group and the model group were not treated with acupuncture for 20 min;. After 14 days of electroacupuncture intervention, the changes of cell count, scar area and cross sectional area of muscle fiber were observed by hematoxylin eosin (HE) staining and Ma Song trichromatic (Masson) staining. The activity of CK and the content of IL-17 in serum were detected by (ELISA), and the expression of IL-17 was detected by immunohistochemistry. Results: after intervention, the inflammatory cell count, scar area and muscle fiber cross-sectional area in model group, electroacupuncture medium group and electroacupuncture Shenshu group were significantly higher than those in control group (P0.01). The number of cells and scar area in EA group and EA group were less than those in model group (P0.01), and the cross sectional area of muscle fiber was larger than that in model group (P0.01P 0.05). After intervention, the expression of IL-17, the content of serum IL-17 and the activity of CK in model group, electroacupuncture group and Shenshu group were significantly higher than those in control group (P0.01). The expression of IL-17, the content of serum IL-17 and the activity of CK were lower in the electroacupuncture group and Shenshu group than those in the model group (P 0.01 P 0.05). Compared with the Shenshu group, the down-regulation of IL-17 was more obvious in the electroacupuncture group (P0.01). Conclusion: electroacupuncture can attenuate inflammatory reaction and promote benign repair of polyfissure muscle by down-regulating the overexpression of serum CK and interleukin-17.
【作者单位】：北京中医药大学针灸推拿学院;广东省第二中医院针灸康复科;北京大学第三医院中医科;
【基金】：国家自然科学基金面上项目:81574052
【分类号】：R245.97

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