隔药饼灸对FD肝郁脾虚模型大鼠胃排空率及下丘脑单胺类神经递质的影响
发布时间：2019-01-29 04:49
【摘要】：目的:观察隔药饼灸对功能性消化不良(FD)肝郁脾虚模型大鼠胃排空率及下丘脑五羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)的影响,探讨隔药饼灸治疗FD可能的作用机制。方法:将50只SD大鼠按照随机数字表法随机分为5组,每组10只,分别为空白组、模型组、隔药饼灸组、艾炷灸组和逍遥散组。空白组予正常饲养,其余4组采用复合病因造模法(慢性束缚应激+过度疲劳+饮食失节+夹尾+摇晃)造模,连续21d;再按分组施加被试因素,分别进行隔药饼灸、艾炷灸和逍遥散灌胃治疗14d。隔药饼灸组、艾炷灸组施灸穴位均分为I组(“肝俞”“脾俞”“胃俞”)和II组(“章门”“期门”“中脘”),两组穴位隔日交替,每穴连续灸4-5壮(约30min);逍遥散组按1ml/100g灌服逍遥散水煎剂。治疗结束后,通过观察大鼠胃肠对营养性半固体糊的排空情况计算大鼠的胃排空率,采用高效液相色谱-荧光法检测大鼠下丘脑中5-HT、DA及NE的含量。结果:1.造模后,大鼠表现为焦躁,倦怠,扎堆角落,皮毛黯淡,粪便时干时稀;大鼠的穿格数、起立数、修饰数与造模前比较均减少(P0.01,或P0.05);2.与空白组比较,模型大鼠胃排空率明显下降(P0.01);与模型组比较,各治疗组大鼠胃排空率较模型组均显著上升(P0.01),但各治疗组组间比较差异无统计学意义(P0.05);3.与空白组比较,模型大鼠下丘脑中5-HT、DA、NE含量均显著下降(P0.01);与模型组比较,艾炷灸组大鼠下丘脑中5-HT、DA、NE含量均有所上升,但差异无统计学意义(P0.05);隔药饼灸组、逍遥散组大鼠下丘脑中5-HT、DA、NE含量显著上升(P0.01),但两组组间比较差异无统计学意意义(P0.05)。结论:隔药饼灸能显著改善FD肝郁脾虚模型大鼠的胃肠动力,提高其胃排空率,同时显著增加FD肝郁脾虚模型大鼠下丘脑中5-HT、DA、NE的含量,从而纠正HPA轴的功能亢进,这可能是其能够有效治疗FD的重要作用机制。
[Abstract]:Objective: to observe the effects of herbal cake moxibustion on gastric emptying rate and hypothalamic serotonin (5-HT) and dopamine (DA), noradrenaline (DA),) (NE) in rats with functional dyspepsia (FD) with liver depression and spleen deficiency. To explore the possible mechanism of moxibustion on FD. Methods: fifty SD rats were randomly divided into 5 groups (10 rats in each group): blank group, model group, moxibustion group and Xiaoyao powder group. The control group was fed with normal diet, and the other 4 groups were made by the method of compound etiology (chronic restraint stress, excessive fatigue diet and tail shaking) for 21 days. Then the patients were treated with moxibustion, moxa cone moxibustion and Xiaoyao powder for 14 days. The moxibustion points of moxibustion group and moxa cone moxibustion group were divided into two groups: group I ("Ganshu", "Peshu", "Weishu") and group II ("Zhangmen", "phengmen", "Zhongwan"). The acupoints of the two groups alternated every other day and each acupoint was continuously moxibustion about 4-5 (about 30min). Xiaoyao Powder group was given Xiaoyao Powder decoction by 1ml/100g. After the treatment, the gastric emptying rate of the rats was calculated by observing the emptying of nutritious semisolid paste. The contents of 5-HTDA and NE in the hypothalamus of rats were detected by HPLC and fluorescence method. Results: 1. After modeling, the rats showed restlessness, burnout, bundled corner, dark fur, dilute dry time of feces, the number of piercing, standing and modification of rats were decreased compared with those before modeling (P0.01, or P0.05); 2. Compared with the blank group, the gastric emptying rate of the model rats decreased significantly (P0.01). Compared with the model group, the gastric emptying rate in each treatment group was significantly higher than that in the model group (P0.01), but there was no significant difference between the treatment groups (P0.05); Compared with the blank group, the contents of 5-HTTDA NE in the hypothalamus of the model rats were significantly decreased (P0.01). Compared with the model group, the hypothalamus of the moxa cone moxibustion group increased the content of 5-HTTDA NE, but the difference was not statistically significant (P0.05). The content of 5-HTTDA NE in hypothalamus of rats in moxibustion group and Xiaoyao powder group increased significantly (P0.01), but there was no significant difference between the two groups (P0.05). Conclusion: moxibustion with herbal cake can significantly improve the gastrointestinal motility and gastric emptying rate of FD rats with liver stagnation and spleen deficiency, and increase the content of 5-HTDDA NE in hypothalamus of rats with FD liver stagnation and spleen deficiency, so as to correct the hyperfunction of HPA axis. This may be an important mechanism for the effective treatment of FD.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245;R-332
本文编号：2417662
[Abstract]:Objective: to observe the effects of herbal cake moxibustion on gastric emptying rate and hypothalamic serotonin (5-HT) and dopamine (DA), noradrenaline (DA),) (NE) in rats with functional dyspepsia (FD) with liver depression and spleen deficiency. To explore the possible mechanism of moxibustion on FD. Methods: fifty SD rats were randomly divided into 5 groups (10 rats in each group): blank group, model group, moxibustion group and Xiaoyao powder group. The control group was fed with normal diet, and the other 4 groups were made by the method of compound etiology (chronic restraint stress, excessive fatigue diet and tail shaking) for 21 days. Then the patients were treated with moxibustion, moxa cone moxibustion and Xiaoyao powder for 14 days. The moxibustion points of moxibustion group and moxa cone moxibustion group were divided into two groups: group I ("Ganshu", "Peshu", "Weishu") and group II ("Zhangmen", "phengmen", "Zhongwan"). The acupoints of the two groups alternated every other day and each acupoint was continuously moxibustion about 4-5 (about 30min). Xiaoyao Powder group was given Xiaoyao Powder decoction by 1ml/100g. After the treatment, the gastric emptying rate of the rats was calculated by observing the emptying of nutritious semisolid paste. The contents of 5-HTDA and NE in the hypothalamus of rats were detected by HPLC and fluorescence method. Results: 1. After modeling, the rats showed restlessness, burnout, bundled corner, dark fur, dilute dry time of feces, the number of piercing, standing and modification of rats were decreased compared with those before modeling (P0.01, or P0.05); 2. Compared with the blank group, the gastric emptying rate of the model rats decreased significantly (P0.01). Compared with the model group, the gastric emptying rate in each treatment group was significantly higher than that in the model group (P0.01), but there was no significant difference between the treatment groups (P0.05); Compared with the blank group, the contents of 5-HTTDA NE in the hypothalamus of the model rats were significantly decreased (P0.01). Compared with the model group, the hypothalamus of the moxa cone moxibustion group increased the content of 5-HTTDA NE, but the difference was not statistically significant (P0.05). The content of 5-HTTDA NE in hypothalamus of rats in moxibustion group and Xiaoyao powder group increased significantly (P0.01), but there was no significant difference between the two groups (P0.05). Conclusion: moxibustion with herbal cake can significantly improve the gastrointestinal motility and gastric emptying rate of FD rats with liver stagnation and spleen deficiency, and increase the content of 5-HTDDA NE in hypothalamus of rats with FD liver stagnation and spleen deficiency, so as to correct the hyperfunction of HPA axis. This may be an important mechanism for the effective treatment of FD.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245;R-332
【参考文献】
相关期刊论文 前10条
1 刘晶;李峰;唐旭东;马捷;白世敬;刘艳阳;;功能性消化不良中医辨证及辨证标准的现代临床文献研究[J];世界中医药;2015年01期
2 叶国燕;;对功能性消化不良患者的临床表现与发病机制分析[J];当代医药论丛;2014年11期
3 傅滟茹;田小平;;隔盐灸治疗功能性消化不良46例[J];河南中医;2014年07期
4 钟文英;王新芳;黄光鸿;;抗抑郁药治疗功能性消化不良的疗效分析[J];现代消化及介入诊疗;2014年03期
5 谢秀俊;熊俊;陈日新;;基于数据挖掘灸法治疗功能性消化不良的临床证据[J];时珍国医国药;2014年05期
6 刘未艾;常小荣;吴清明;刘密;张国山;石佳;章海凤;;隔药饼灸对功能性胃肠病肝郁脾虚模型大鼠SP与肠道敏感性的影响[J];世界华人消化杂志;2014年12期
7 色音白乙拉;;蒙医艾灸法治疗功能性消化不良体会[J];中国民族民间医药;2014年02期
8 刘杨春;;艾灸治疗功能性消化不良综合征30例[J];中国中医药现代远程教育;2014年02期
9 徐碧林;吴伟;张伟清;;温针灸对脾胃气虚型功能性消化不良的疗效观察[J];浙江中医杂志;2014年01期
10 章海凤;刘未艾;常小荣;刘密;张国山;石佳;;隔药饼灸对功能性胃肠病(肝郁脾虚证)大鼠海马和杏仁核AMPA受体亚基表达的影响[J];世界华人消化杂志;2013年15期
，本文编号：2417662
本文链接：https://www.wllwen.com/zhongyixuelunwen/2417662.html
最近更新
教材专著
